Antibiotic resistance and typing of agr locus in Staphylococcus aureus isolated from clinical samples in Sanandaj, Western Iran
Objective(s): Infections by Staphylococcus aureus remain an important health problem. The aims were to detect mecA, staphylococcal cassette chromosome mec (SCCmec), accessory gene regulator (agr), and integrons in S. aureus and to investigate the relationship of agr types with antibiotic resistance...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_16395_8dd367e7f50dc82086f2416a17c3c21b.pdf |
| _version_ | 1811295340939182080 |
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| author | Samira Saedi Safoura Derakhshan Ebrahim Ghaderi |
| author_facet | Samira Saedi Safoura Derakhshan Ebrahim Ghaderi |
| author_sort | Samira Saedi |
| collection | DOAJ |
| description | Objective(s): Infections by Staphylococcus aureus remain an important health problem. The aims were to detect mecA, staphylococcal cassette chromosome mec (SCCmec), accessory gene regulator (agr), and integrons in S. aureus and to investigate the relationship of agr types with antibiotic resistance of isolates. <br />Materials and Methods: In this cross-sectional study, 70 S. aureus isolates were collected between December 2017 and May 2018 from clinical specimens of patients in two hospitals of Sanandaj, western Iran. Susceptibility was determined by disk diffusion for 9 antibiotics and by vancomycin E test. The mecA, classes 1-3 integrons, SCCmec I-V, and agr I-IV were detected by polymerase chain reaction. A P-value<0.05 was considered significant.<br />Results: The most effective antibiotics were linezolid, vancomycin, and trimethoprim-sulfamethoxazole (above 90% sensitivity). Of the 70 isolates, 17.1% were methicillin-resistant S. aureus (MRSA), 8.6% carried class 1 integron, 11.4% carried mecA, 17.1% carried agr I, and 30% carried agr III. SCCmec III and SCCmecV were detected. An association was found between resistance to certain antibiotics and the presence of agr I (P-value<0.05). Conversely, the prevalence of agr III in susceptible strains was higher than non-susceptible strains, and no MRSA isolates belonged to agr III (P-value<0.05).Conclusion: These data suggest that agr activity may influence the resistance of S. aureus to antibiotics. Although the prevalence of mecA and integron was relatively low, the identification of such strains calls for serious health concerns; thus highlights the need to monitor drug resistance in S. aureus. |
| first_indexed | 2024-04-13T05:31:00Z |
| format | Article |
| id | doaj.art-357b2dbe910b4c3bbc251a2ce268c94b |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T05:31:00Z |
| publishDate | 2020-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-357b2dbe910b4c3bbc251a2ce268c94b2022-12-22T03:00:26ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-10-0123101307131410.22038/ijbms.2020.46064.1066116395Antibiotic resistance and typing of agr locus in Staphylococcus aureus isolated from clinical samples in Sanandaj, Western IranSamira Saedi0Safoura Derakhshan1Ebrahim Ghaderi2Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, IranLiver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, IranZoonoses Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, IranObjective(s): Infections by Staphylococcus aureus remain an important health problem. The aims were to detect mecA, staphylococcal cassette chromosome mec (SCCmec), accessory gene regulator (agr), and integrons in S. aureus and to investigate the relationship of agr types with antibiotic resistance of isolates. <br />Materials and Methods: In this cross-sectional study, 70 S. aureus isolates were collected between December 2017 and May 2018 from clinical specimens of patients in two hospitals of Sanandaj, western Iran. Susceptibility was determined by disk diffusion for 9 antibiotics and by vancomycin E test. The mecA, classes 1-3 integrons, SCCmec I-V, and agr I-IV were detected by polymerase chain reaction. A P-value<0.05 was considered significant.<br />Results: The most effective antibiotics were linezolid, vancomycin, and trimethoprim-sulfamethoxazole (above 90% sensitivity). Of the 70 isolates, 17.1% were methicillin-resistant S. aureus (MRSA), 8.6% carried class 1 integron, 11.4% carried mecA, 17.1% carried agr I, and 30% carried agr III. SCCmec III and SCCmecV were detected. An association was found between resistance to certain antibiotics and the presence of agr I (P-value<0.05). Conversely, the prevalence of agr III in susceptible strains was higher than non-susceptible strains, and no MRSA isolates belonged to agr III (P-value<0.05).Conclusion: These data suggest that agr activity may influence the resistance of S. aureus to antibiotics. Although the prevalence of mecA and integron was relatively low, the identification of such strains calls for serious health concerns; thus highlights the need to monitor drug resistance in S. aureus.http://ijbms.mums.ac.ir/article_16395_8dd367e7f50dc82086f2416a17c3c21b.pdfagr proteindrug resistanceiranmethicillinstaphylococcus aureus |
| spellingShingle | Samira Saedi Safoura Derakhshan Ebrahim Ghaderi Antibiotic resistance and typing of agr locus in Staphylococcus aureus isolated from clinical samples in Sanandaj, Western Iran Iranian Journal of Basic Medical Sciences agr protein drug resistance iran methicillin staphylococcus aureus |
| title | Antibiotic resistance and typing of agr locus in Staphylococcus aureus isolated from clinical samples in Sanandaj, Western Iran |
| title_full | Antibiotic resistance and typing of agr locus in Staphylococcus aureus isolated from clinical samples in Sanandaj, Western Iran |
| title_fullStr | Antibiotic resistance and typing of agr locus in Staphylococcus aureus isolated from clinical samples in Sanandaj, Western Iran |
| title_full_unstemmed | Antibiotic resistance and typing of agr locus in Staphylococcus aureus isolated from clinical samples in Sanandaj, Western Iran |
| title_short | Antibiotic resistance and typing of agr locus in Staphylococcus aureus isolated from clinical samples in Sanandaj, Western Iran |
| title_sort | antibiotic resistance and typing of agr locus in staphylococcus aureus isolated from clinical samples in sanandaj western iran |
| topic | agr protein drug resistance iran methicillin staphylococcus aureus |
| url | http://ijbms.mums.ac.ir/article_16395_8dd367e7f50dc82086f2416a17c3c21b.pdf |
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