Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro
<i>Opsariichthys bidens</i> belongs to the family <i>Cyprinidae</i> and is a small freshwater economic fish widely distributed in China. In recent years, the natural resources of <i>O. bidens</i> have been drastically reduced due to overfishing and the destruction...
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MDPI AG
2022-07-01
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| author | Xiao Chen Yuting Kan Ying Zhong Muhammad Jawad Wenbo Wei Kaiyan Gu Lang Gui Mingyou Li |
| author_facet | Xiao Chen Yuting Kan Ying Zhong Muhammad Jawad Wenbo Wei Kaiyan Gu Lang Gui Mingyou Li |
| author_sort | Xiao Chen |
| collection | DOAJ |
| description | <i>Opsariichthys bidens</i> belongs to the family <i>Cyprinidae</i> and is a small freshwater economic fish widely distributed in China. In recent years, the natural resources of <i>O. bidens</i> have been drastically reduced due to overfishing and the destruction of the water environment. The in vitro culture and long-term preservation of germ stem cells are the key technologies to keep genetic resources from degeneration. However, except for the establishment of the first long-term cultured medaka spermatogonia cell line (SSC) capable of producing sperm in vitro in 2004, no other long-term cultured SSC line has been found in other fish species. In this study, we successfully established another long-term-cultured spermatogonial stem cell line from <i>Opsariichthys bidens</i> (ObSSC). After more than 2 years of culture, ObSSC had a diploid karyotype and stable growth, with the typical gene expression patterns of SSC. Under in vitro culture, ObSSC could be induced to differentiate into sperm and other different types of somatic cells. In vivo, ObSSC could differentiate into different cells of three germ layers upon being transplanted into zebrafish embryos. Our research helps to explore the potential and regulation mechanism of fish SSC differentiation and spermatogenesis in vitro, provides a new way for solving the problem of fish genetic resource degradation and lays a foundation for further research on fish germ cell transplantation. |
| first_indexed | 2024-03-09T10:22:04Z |
| format | Article |
| id | doaj.art-357fa997f1264f038664eac304599559 |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T10:22:04Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-357fa997f1264f038664eac3045995592023-12-01T21:54:44ZengMDPI AGBiology2079-77372022-07-01117106910.3390/biology11071069Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In VitroXiao Chen0Yuting Kan1Ying Zhong2Muhammad Jawad3Wenbo Wei4Kaiyan Gu5Lang Gui6Mingyou Li7Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China<i>Opsariichthys bidens</i> belongs to the family <i>Cyprinidae</i> and is a small freshwater economic fish widely distributed in China. In recent years, the natural resources of <i>O. bidens</i> have been drastically reduced due to overfishing and the destruction of the water environment. The in vitro culture and long-term preservation of germ stem cells are the key technologies to keep genetic resources from degeneration. However, except for the establishment of the first long-term cultured medaka spermatogonia cell line (SSC) capable of producing sperm in vitro in 2004, no other long-term cultured SSC line has been found in other fish species. In this study, we successfully established another long-term-cultured spermatogonial stem cell line from <i>Opsariichthys bidens</i> (ObSSC). After more than 2 years of culture, ObSSC had a diploid karyotype and stable growth, with the typical gene expression patterns of SSC. Under in vitro culture, ObSSC could be induced to differentiate into sperm and other different types of somatic cells. In vivo, ObSSC could differentiate into different cells of three germ layers upon being transplanted into zebrafish embryos. Our research helps to explore the potential and regulation mechanism of fish SSC differentiation and spermatogenesis in vitro, provides a new way for solving the problem of fish genetic resource degradation and lays a foundation for further research on fish germ cell transplantation.https://www.mdpi.com/2079-7737/11/7/1069<i>Opsariichthys bidens</i>spermatogonia stem cell linein vitro spermatogenesisdifferentiationcryopreservationcell transplantation technology |
| spellingShingle | Xiao Chen Yuting Kan Ying Zhong Muhammad Jawad Wenbo Wei Kaiyan Gu Lang Gui Mingyou Li Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro Biology <i>Opsariichthys bidens</i> spermatogonia stem cell line in vitro spermatogenesis differentiation cryopreservation cell transplantation technology |
| title | Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro |
| title_full | Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro |
| title_fullStr | Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro |
| title_full_unstemmed | Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro |
| title_short | Generation of a Normal Long-Term-Cultured Chinese Hook Snout Carp Spermatogonial Stem Cell Line Capable of Sperm Production In Vitro |
| title_sort | generation of a normal long term cultured chinese hook snout carp spermatogonial stem cell line capable of sperm production in vitro |
| topic | <i>Opsariichthys bidens</i> spermatogonia stem cell line in vitro spermatogenesis differentiation cryopreservation cell transplantation technology |
| url | https://www.mdpi.com/2079-7737/11/7/1069 |
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