Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cells
Breast cancer (BC) remains the leading cause of death in women worldwide, despite the improvements of cancer screening and treatment methods. Recently, development of novel anticancer drugs for the improved prevention and treatment of BC is in the center of research. The anticancer effects of bromel...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Medical Signals and Sensors |
| Subjects: | |
| Online Access: | http://www.jmss.mui.ac.ir/article.asp?issn=2228-7477;year=2019;volume=9;issue=1;spage=68;epage=74;aulast=Raeisi |
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| author | Farzaneh Raeisi Daryoush Shahbazi-Gahrouei Elham Raeisi Esfandiar Heidarian |
| author_facet | Farzaneh Raeisi Daryoush Shahbazi-Gahrouei Elham Raeisi Esfandiar Heidarian |
| author_sort | Farzaneh Raeisi |
| collection | DOAJ |
| description | Breast cancer (BC) remains the leading cause of death in women worldwide, despite the improvements of cancer screening and treatment methods. Recently, development of novel anticancer drugs for the improved prevention and treatment of BC is in the center of research. The anticancer effects of bromelain, as enzyme extract derived from the pineapples, contains chemicals that interfere with the growth of tumor cells. The aim of this study was to evaluate the effect of radiosensitizing of bromelain in 4T1 BC cells. This investigation utilized the 3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide assay to characterize the cytotoxicity of bromelain. Colony formation method was used to establish the truth of the capability of bromelain to make sensitive to radiation therapy. Flowcytometry performed to define the contribution the apoptosis effect to bromelain mediated radiosensitization of 4T1 cells. Bromelain reduced growth and proliferation of 4T1 cell as a concentration-dependence manner significantly. The survival of 4T1 cancer cells was decreased after combined treatment in a number and size-dependent manner with regard to the control group (P < 0.05). Combination of bromelain with radiation does not influence 4T1 cell apoptosis. The results suggested that bromelain can inhibit the growth and proliferation and reduce survival of 4T1 BC cells and might be used as a candidate radiosensitizer in BC patient. |
| first_indexed | 2024-04-14T06:08:19Z |
| format | Article |
| id | doaj.art-358619f600304c7aa6f22c49b35b6aac |
| institution | Directory Open Access Journal |
| issn | 2228-7477 |
| language | English |
| last_indexed | 2024-04-14T06:08:19Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Medical Signals and Sensors |
| spelling | doaj.art-358619f600304c7aa6f22c49b35b6aac2022-12-22T02:08:28ZengWolters Kluwer Medknow PublicationsJournal of Medical Signals and Sensors2228-74772019-01-0191687410.4103/jmss.JMSS_25_18Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cellsFarzaneh RaeisiDaryoush Shahbazi-GahroueiElham RaeisiEsfandiar HeidarianBreast cancer (BC) remains the leading cause of death in women worldwide, despite the improvements of cancer screening and treatment methods. Recently, development of novel anticancer drugs for the improved prevention and treatment of BC is in the center of research. The anticancer effects of bromelain, as enzyme extract derived from the pineapples, contains chemicals that interfere with the growth of tumor cells. The aim of this study was to evaluate the effect of radiosensitizing of bromelain in 4T1 BC cells. This investigation utilized the 3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide assay to characterize the cytotoxicity of bromelain. Colony formation method was used to establish the truth of the capability of bromelain to make sensitive to radiation therapy. Flowcytometry performed to define the contribution the apoptosis effect to bromelain mediated radiosensitization of 4T1 cells. Bromelain reduced growth and proliferation of 4T1 cell as a concentration-dependence manner significantly. The survival of 4T1 cancer cells was decreased after combined treatment in a number and size-dependent manner with regard to the control group (P < 0.05). Combination of bromelain with radiation does not influence 4T1 cell apoptosis. The results suggested that bromelain can inhibit the growth and proliferation and reduce survival of 4T1 BC cells and might be used as a candidate radiosensitizer in BC patient.http://www.jmss.mui.ac.ir/article.asp?issn=2228-7477;year=2019;volume=9;issue=1;spage=68;epage=74;aulast=Raeisi4T1breast cancerbromelainradiosensitizing |
| spellingShingle | Farzaneh Raeisi Daryoush Shahbazi-Gahrouei Elham Raeisi Esfandiar Heidarian Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cells Journal of Medical Signals and Sensors 4T1 breast cancer bromelain radiosensitizing |
| title | Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cells |
| title_full | Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cells |
| title_fullStr | Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cells |
| title_full_unstemmed | Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cells |
| title_short | Evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4T1 breast cancer cells |
| title_sort | evaluation of the radiosensitizing potency of bromelain for radiation therapy of 4t1 breast cancer cells |
| topic | 4T1 breast cancer bromelain radiosensitizing |
| url | http://www.jmss.mui.ac.ir/article.asp?issn=2228-7477;year=2019;volume=9;issue=1;spage=68;epage=74;aulast=Raeisi |
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