Efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditions
The influence of temperature conditions on the level of damage of rabbit erythrocytes under posthypertonic shock and the level of their protection by amphiphilic compounds was investigated. We observed the maximum cell damage at 0°C. When the temperature increased to 20°C, the level of hemolysis dec...
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| Format: | Article |
| Language: | English |
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Institute of Animal Biology NAAS
2022-12-01
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| Series: | Біологія тварин |
| Subjects: | |
| Online Access: | https://aminbiol.com.ua/index.php/archive/193-archive/bt-24-4-2022/2046-efficiency-of-amphiphilic-compounds-in-rabbit-erythrocytes-posthypertonic-shock-depending-on-temperature-conditions |
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| author | O. E. Nipot N. A. Ershova N. M. Shpakova S. S. Ershov O. O. Shapkina |
| author_facet | O. E. Nipot N. A. Ershova N. M. Shpakova S. S. Ershov O. O. Shapkina |
| author_sort | O. E. Nipot |
| collection | DOAJ |
| description | The influence of temperature conditions on the level of damage of rabbit erythrocytes under posthypertonic shock and the level of their protection by amphiphilic compounds was investigated. We observed the maximum cell damage at 0°C. When the temperature increased to 20°C, the level of hemolysis decreased by 1.8 times. Further increase in temperature up to 37°C did not lead to a decrease in damage. The investigated amphiphilic compounds at 0°C and 10°C effectively protected rabbit erythrocytes from posthypertonic shock. Reduction of hemolytic damage was 2–3 times. At 20°C amphiphilic compounds did not affect the level of cell damage, and at 30°C and 37°C they increased it. The existence of temperature dependence of posthypertonic damage showed the involvement of the phospholipid component of the erythrocyte membrane in the process. Lower temperature is characterized by greater orderliness of lipids, its increase is accompanied by disorder and increased fluidity, and hence elasticity of the membrane. As a result, erythrocyte damage in posthypertonic shock is less at the temperature of 20–37°C. The addition of amphiphilic compounds at 0 and 10°C acts similarly to increasing the temperature, disorganizes the bilayer, increases the elasticity of the membrane and reduces damage during the transfer from hypertonic to isotonic solution. Above 20°C, the introduction of amphiphilic compounds leads not only to disorder, but also to the formation of mixed micelles consisting of phospholipids and amphiphilic molecules. This disrupts the bilayer, gives it instability and leads to increased damage of erythrocytes. |
| first_indexed | 2024-03-08T18:56:41Z |
| format | Article |
| id | doaj.art-3586d53cdf4a4eeb826437eef9a81d34 |
| institution | Directory Open Access Journal |
| issn | 1681-0015 2313-2191 |
| language | English |
| last_indexed | 2024-03-08T18:56:41Z |
| publishDate | 2022-12-01 |
| publisher | Institute of Animal Biology NAAS |
| record_format | Article |
| series | Біологія тварин |
| spelling | doaj.art-3586d53cdf4a4eeb826437eef9a81d342023-12-28T11:19:13ZengInstitute of Animal Biology NAASБіологія тварин1681-00152313-21912022-12-0124481110.15407/animbiol24.04.008Efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditionsO. E. Nipot0https://orcid.org/0000-0003-2877-8896N. A. Ershova1https://orcid.org/0000-0001-9332-6752N. M. Shpakova2https://orcid.org/0000-0002-0148-7522S. S. Ershov3https://orcid.org/0000-0002-6136-1825O. O. Shapkina4https://orcid.org/0000-0002-0595-7130Institute for Problems of Cryobiology and Cryomedicine NAS of UkraineInstitute for Problems of Cryobiology and Cryomedicine NAS of UkraineInstitute for Problems of Cryobiology and Cryomedicine NAS of UkraineInstitute for Problems of Cryobiology and Cryomedicine NAS of UkraineInstitute for Problems of Cryobiology and Cryomedicine NAS of UkraineThe influence of temperature conditions on the level of damage of rabbit erythrocytes under posthypertonic shock and the level of their protection by amphiphilic compounds was investigated. We observed the maximum cell damage at 0°C. When the temperature increased to 20°C, the level of hemolysis decreased by 1.8 times. Further increase in temperature up to 37°C did not lead to a decrease in damage. The investigated amphiphilic compounds at 0°C and 10°C effectively protected rabbit erythrocytes from posthypertonic shock. Reduction of hemolytic damage was 2–3 times. At 20°C amphiphilic compounds did not affect the level of cell damage, and at 30°C and 37°C they increased it. The existence of temperature dependence of posthypertonic damage showed the involvement of the phospholipid component of the erythrocyte membrane in the process. Lower temperature is characterized by greater orderliness of lipids, its increase is accompanied by disorder and increased fluidity, and hence elasticity of the membrane. As a result, erythrocyte damage in posthypertonic shock is less at the temperature of 20–37°C. The addition of amphiphilic compounds at 0 and 10°C acts similarly to increasing the temperature, disorganizes the bilayer, increases the elasticity of the membrane and reduces damage during the transfer from hypertonic to isotonic solution. Above 20°C, the introduction of amphiphilic compounds leads not only to disorder, but also to the formation of mixed micelles consisting of phospholipids and amphiphilic molecules. This disrupts the bilayer, gives it instability and leads to increased damage of erythrocytes.https://aminbiol.com.ua/index.php/archive/193-archive/bt-24-4-2022/2046-efficiency-of-amphiphilic-compounds-in-rabbit-erythrocytes-posthypertonic-shock-depending-on-temperature-conditionsеритроцити кроликаамфіфільні сполукипостгіпертонічний шоктемператураrabbit erythrocytesamphiphilic compoundsposthypertonic shocktemperature |
| spellingShingle | O. E. Nipot N. A. Ershova N. M. Shpakova S. S. Ershov O. O. Shapkina Efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditions Біологія тварин еритроцити кролика амфіфільні сполуки постгіпертонічний шок температура rabbit erythrocytes amphiphilic compounds posthypertonic shock temperature |
| title | Efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditions |
| title_full | Efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditions |
| title_fullStr | Efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditions |
| title_full_unstemmed | Efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditions |
| title_short | Efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditions |
| title_sort | efficiency of amphiphilic compounds in rabbit erythrocytes posthypertonic shock depending on temperature conditions |
| topic | еритроцити кролика амфіфільні сполуки постгіпертонічний шок температура rabbit erythrocytes amphiphilic compounds posthypertonic shock temperature |
| url | https://aminbiol.com.ua/index.php/archive/193-archive/bt-24-4-2022/2046-efficiency-of-amphiphilic-compounds-in-rabbit-erythrocytes-posthypertonic-shock-depending-on-temperature-conditions |
| work_keys_str_mv | AT oenipot efficiencyofamphiphiliccompoundsinrabbiterythrocytesposthypertonicshockdependingontemperatureconditions AT naershova efficiencyofamphiphiliccompoundsinrabbiterythrocytesposthypertonicshockdependingontemperatureconditions AT nmshpakova efficiencyofamphiphiliccompoundsinrabbiterythrocytesposthypertonicshockdependingontemperatureconditions AT ssershov efficiencyofamphiphiliccompoundsinrabbiterythrocytesposthypertonicshockdependingontemperatureconditions AT ooshapkina efficiencyofamphiphiliccompoundsinrabbiterythrocytesposthypertonicshockdependingontemperatureconditions |