ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds
Recently, the plant hormone abscisic acid (ABA) has been implicated as a key player in the regulation of endodormancy (ED) in grapevine buds (Vitis vinifera L). In this study, we show that in the vine, the expression of genes related to the biosynthesis of ABA (VvNCED1; VvNCED2) and the content of A...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00812/full |
| _version_ | 1811220656970268672 |
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| author | Ricardo Vergara Ricardo Vergara Ximena Noriega Karla Aravena Humberto Prieto Francisco J. Pérez |
| author_facet | Ricardo Vergara Ricardo Vergara Ximena Noriega Karla Aravena Humberto Prieto Francisco J. Pérez |
| author_sort | Ricardo Vergara |
| collection | DOAJ |
| description | Recently, the plant hormone abscisic acid (ABA) has been implicated as a key player in the regulation of endodormancy (ED) in grapevine buds (Vitis vinifera L). In this study, we show that in the vine, the expression of genes related to the biosynthesis of ABA (VvNCED1; VvNCED2) and the content of ABA are significantly higher in the latent bud than at the shoot apex, while the expression of an ABA catabolic gene (VvA8H3) showed no significant difference between either organ. A negative correlation between the content of ABA and transcript levels of cell cycle genes (CCG) was found in both tissues. This result suggested that ABA may negatively regulate the expression of CCG in meristematic tissues of grapevines. To test this proposition, the effect of ABA on the expression of CCG was analyzed in two meristematic tissues of the vine: somatic embryos and shoot apexes. The results indicated that cell cycle progression is repressed by ABA in both organs, since it down-regulated the expression of genes encoding cyclin-dependent kinases (VvCDKB1, VvCDKB2) and genes encoding cyclins of type A (VvCYCA1, VvCYCA2, VvCYCA3), B (VvCYCB), and D (VvCYCD3.2a) and up-regulated the expression of VvICK5, a gene encoding an inhibitor of CDKs. During ED, the content of ABA increased, and the expression of CCG decreased. Moreover, the dormancy-breaking compound hydrogen cyanamide (HC) reduced the content of ABA and up-regulated the expression of CCG, this last effect was abolished when HC and ABA were co-applied. Taken together, these results suggest that ABA-mediated repression of CCG transcription may be part of the mechanism through which ABA modulates the development of ED in grapevine buds. |
| first_indexed | 2024-04-12T07:45:36Z |
| format | Article |
| id | doaj.art-3588a4ab83784b12a3601bd055c9e61d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T07:45:36Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3588a4ab83784b12a3601bd055c9e61d2022-12-22T03:41:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-05-01810.3389/fpls.2017.00812227832ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine BudsRicardo Vergara0Ricardo Vergara1Ximena Noriega2Karla Aravena3Humberto Prieto4Francisco J. Pérez5Laboratorio de Bioquímica Vegetal, Facultad de Ciencias, Universidad de ChileSantiago, ChilePrograma Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Universidad de ChileSantiago, ChileLaboratorio de Bioquímica Vegetal, Facultad de Ciencias, Universidad de ChileSantiago, ChileLaboratorio de Bioquímica Vegetal, Facultad de Ciencias, Universidad de ChileSantiago, ChileLaboratorio de Biotecnología Vegetal, Instituto de Investigaciones Agropecuarias, La PlatinaSantiago, ChileLaboratorio de Bioquímica Vegetal, Facultad de Ciencias, Universidad de ChileSantiago, ChileRecently, the plant hormone abscisic acid (ABA) has been implicated as a key player in the regulation of endodormancy (ED) in grapevine buds (Vitis vinifera L). In this study, we show that in the vine, the expression of genes related to the biosynthesis of ABA (VvNCED1; VvNCED2) and the content of ABA are significantly higher in the latent bud than at the shoot apex, while the expression of an ABA catabolic gene (VvA8H3) showed no significant difference between either organ. A negative correlation between the content of ABA and transcript levels of cell cycle genes (CCG) was found in both tissues. This result suggested that ABA may negatively regulate the expression of CCG in meristematic tissues of grapevines. To test this proposition, the effect of ABA on the expression of CCG was analyzed in two meristematic tissues of the vine: somatic embryos and shoot apexes. The results indicated that cell cycle progression is repressed by ABA in both organs, since it down-regulated the expression of genes encoding cyclin-dependent kinases (VvCDKB1, VvCDKB2) and genes encoding cyclins of type A (VvCYCA1, VvCYCA2, VvCYCA3), B (VvCYCB), and D (VvCYCD3.2a) and up-regulated the expression of VvICK5, a gene encoding an inhibitor of CDKs. During ED, the content of ABA increased, and the expression of CCG decreased. Moreover, the dormancy-breaking compound hydrogen cyanamide (HC) reduced the content of ABA and up-regulated the expression of CCG, this last effect was abolished when HC and ABA were co-applied. Taken together, these results suggest that ABA-mediated repression of CCG transcription may be part of the mechanism through which ABA modulates the development of ED in grapevine buds.http://journal.frontiersin.org/article/10.3389/fpls.2017.00812/fullabscisic acid (ABA)cell cycle genesendodormancygrapevine buds |
| spellingShingle | Ricardo Vergara Ricardo Vergara Ximena Noriega Karla Aravena Humberto Prieto Francisco J. Pérez ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds Frontiers in Plant Science abscisic acid (ABA) cell cycle genes endodormancy grapevine buds |
| title | ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds |
| title_full | ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds |
| title_fullStr | ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds |
| title_full_unstemmed | ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds |
| title_short | ABA Represses the Expression of Cell Cycle Genes and May Modulate the Development of Endodormancy in Grapevine Buds |
| title_sort | aba represses the expression of cell cycle genes and may modulate the development of endodormancy in grapevine buds |
| topic | abscisic acid (ABA) cell cycle genes endodormancy grapevine buds |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00812/full |
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