Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir <i>Abies beshanzuensis</i>
<i>Abies beshanzuensis</i>, an extremely rare and critically endangered plant with only three wild adult trees globally, is strongly mycorrhizal-dependent, leading to difficulties in protection and artificial breeding without symbiosis. Root hair morphogenesis plays an important role in...
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MDPI AG
2023-01-01
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| author | Bin Liu Ke Liu Xiaorong Chen Duohong Xiao Tingjin Wang Yang Yang Hui Shuai Sumei Wu Lu Yuan Liping Chen |
| author_facet | Bin Liu Ke Liu Xiaorong Chen Duohong Xiao Tingjin Wang Yang Yang Hui Shuai Sumei Wu Lu Yuan Liping Chen |
| author_sort | Bin Liu |
| collection | DOAJ |
| description | <i>Abies beshanzuensis</i>, an extremely rare and critically endangered plant with only three wild adult trees globally, is strongly mycorrhizal-dependent, leading to difficulties in protection and artificial breeding without symbiosis. Root hair morphogenesis plays an important role in the survival of mycorrhizal symbionts. Due to the lack of an effective genome and transcriptome of <i>A. beshanzuensis</i>, the molecular signals involved in the root hair development remain unknown, which hinders its endangered mechanism analysis and protection. Herein, transcriptomes of radicles with root hair (RH1) and without root hair (RH0) from <i>A. beshanzuensis</i> in vitro plantlets were primarily established. Functional annotation and differentially expressed gene (DEG) analysis showed that the two phenotypes have highly differentially expressed gene clusters. Transcriptome divergence identified hormone and sugar signaling primarily involved in root hair morphogenesis of <i>A. beshanzuensis</i>. Weighted correlation network analysis (WGCNA) coupled with quantitative real-time PCR (qRT-PCR) found that two hormone–sucrose–root hair modules were linked by <i>IAA17</i>, and <i>SUS</i> was positioned in the center of the regulation network, co-expressed with <i>SRK2E</i> in hormone transduction and key genes related to root hair morphogenesis. Our results contribute to better understanding of the molecular mechanisms of root hair development and offer new insights into deciphering the survival mechanism of <i>A. beshanzuensis</i> and other endangered species, utilizing root hair as a compensatory strategy instead of poor mycorrhizal growth. |
| first_indexed | 2024-03-09T11:25:08Z |
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| last_indexed | 2024-03-09T11:25:08Z |
| publishDate | 2023-01-01 |
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| series | Plants |
| spelling | doaj.art-3588b259f43542fdbe5210a48c7edb4d2023-12-01T00:03:53ZengMDPI AGPlants2223-77472023-01-0112227610.3390/plants12020276Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir <i>Abies beshanzuensis</i>Bin Liu0Ke Liu1Xiaorong Chen2Duohong Xiao3Tingjin Wang4Yang Yang5Hui Shuai6Sumei Wu7Lu Yuan8Liping Chen9Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaQingyuan Conservation Center of Qianjiangyuan-Baishanzu National Park, Qingyuan 323800, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaQingyuan Conservation Center of Qianjiangyuan-Baishanzu National Park, Qingyuan 323800, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China<i>Abies beshanzuensis</i>, an extremely rare and critically endangered plant with only three wild adult trees globally, is strongly mycorrhizal-dependent, leading to difficulties in protection and artificial breeding without symbiosis. Root hair morphogenesis plays an important role in the survival of mycorrhizal symbionts. Due to the lack of an effective genome and transcriptome of <i>A. beshanzuensis</i>, the molecular signals involved in the root hair development remain unknown, which hinders its endangered mechanism analysis and protection. Herein, transcriptomes of radicles with root hair (RH1) and without root hair (RH0) from <i>A. beshanzuensis</i> in vitro plantlets were primarily established. Functional annotation and differentially expressed gene (DEG) analysis showed that the two phenotypes have highly differentially expressed gene clusters. Transcriptome divergence identified hormone and sugar signaling primarily involved in root hair morphogenesis of <i>A. beshanzuensis</i>. Weighted correlation network analysis (WGCNA) coupled with quantitative real-time PCR (qRT-PCR) found that two hormone–sucrose–root hair modules were linked by <i>IAA17</i>, and <i>SUS</i> was positioned in the center of the regulation network, co-expressed with <i>SRK2E</i> in hormone transduction and key genes related to root hair morphogenesis. Our results contribute to better understanding of the molecular mechanisms of root hair development and offer new insights into deciphering the survival mechanism of <i>A. beshanzuensis</i> and other endangered species, utilizing root hair as a compensatory strategy instead of poor mycorrhizal growth.https://www.mdpi.com/2223-7747/12/2/276<i>Abies beshanzuensis</i>in vitro plantletscomparative transcriptomeroot hair morphogenesissugar metabolismhormone signaling |
| spellingShingle | Bin Liu Ke Liu Xiaorong Chen Duohong Xiao Tingjin Wang Yang Yang Hui Shuai Sumei Wu Lu Yuan Liping Chen Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir <i>Abies beshanzuensis</i> Plants <i>Abies beshanzuensis</i> in vitro plantlets comparative transcriptome root hair morphogenesis sugar metabolism hormone signaling |
| title | Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir <i>Abies beshanzuensis</i> |
| title_full | Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir <i>Abies beshanzuensis</i> |
| title_fullStr | Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir <i>Abies beshanzuensis</i> |
| title_full_unstemmed | Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir <i>Abies beshanzuensis</i> |
| title_short | Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir <i>Abies beshanzuensis</i> |
| title_sort | comparative transcriptome analysis reveals the interaction of sugar and hormone metabolism involved in the root hair morphogenesis of the endangered fir i abies beshanzuensis i |
| topic | <i>Abies beshanzuensis</i> in vitro plantlets comparative transcriptome root hair morphogenesis sugar metabolism hormone signaling |
| url | https://www.mdpi.com/2223-7747/12/2/276 |
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