Molecular Epidemiology of Aminoglycosides Resistance in Acinetobacter Spp. with Emergence of Multidrug-Resistant Strains
Background: Acinetobacter spp. is characterized as an important nosocomial pathogen and increasing antimicrobial resistance. Our aim was to evaluate antimicrobial susceptibility and aminoglycosides resistance genes of Acinetobacter spp. isolated from hospitalized patients.Methods: Sixty isolates wer...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2010-06-01
|
| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/15622.pdf&manuscript_id=15622 |
| _version_ | 1818879758517141504 |
|---|---|
| author | MH Nazem Shirazi Gh Shajari R Kheltabadi Farahani R Moniri A Ghasemi |
| author_facet | MH Nazem Shirazi Gh Shajari R Kheltabadi Farahani R Moniri A Ghasemi |
| author_sort | MH Nazem Shirazi |
| collection | DOAJ |
| description | Background: Acinetobacter spp. is characterized as an important nosocomial pathogen and increasing antimicrobial resistance. Our aim was to evaluate antimicrobial susceptibility and aminoglycosides resistance genes of Acinetobacter spp. isolated from hospitalized patients.Methods: Sixty isolates were identified as Acinetobacter species. The isolates were tested for antibiotic resistance by disc diffusion method for 12 antimicrobials. The presence of aphA6, aacC1 aadA1, and aadB genes were detected using PCR.Results: From the isolated Acinetobacter spp. the highest resistance rate showed against amikacin, tobramycin, and ceftazidim, respectively; while isolated bacteria were more sensitive to ampicillic/subactam. More than 66% of the isolates were resistant to at least three classes of antibiotics, and 27.5% of MDR strains were resistant to all seven tested classes of antimicrobials. The higher MDR rate presented in bacteria isolated from the ICU and blood samples. More than 60% of the MDR bacteria were resistance to amikacin, ceftazidim, ciprofloxacin, piperacillin/tazobactam, doxycycline, tobramycin and levofloxacin. Also, more than 60% of the isolates contained phosphotransferase aphA6, and acetyltransferase genes aacC1, but adenylyltransferase genes aadA1 (41.7%), and aadB (3.3%) were less prominent. 21.7% of the strains contain three aminoglycoside resistance genes (aphA6, aacC1 and aadA1).Conclusion: The rising trend of resistance to aminoglycosides poses an alarming threat to treatment of such infections. The findings showed that clinical isolates of Acinetobacter spp. in our hospital carrying various kinds of aminoglycoside resistance genes. |
| first_indexed | 2024-12-19T14:35:10Z |
| format | Article |
| id | doaj.art-3589015238a04492ae4d9ea020102697 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 |
| language | English |
| last_indexed | 2024-12-19T14:35:10Z |
| publishDate | 2010-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-3589015238a04492ae4d9ea0201026972022-12-21T20:17:19ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852010-06-013926368Molecular Epidemiology of Aminoglycosides Resistance in Acinetobacter Spp. with Emergence of Multidrug-Resistant StrainsMH Nazem ShiraziGh ShajariR Kheltabadi FarahaniR MoniriA GhasemiBackground: Acinetobacter spp. is characterized as an important nosocomial pathogen and increasing antimicrobial resistance. Our aim was to evaluate antimicrobial susceptibility and aminoglycosides resistance genes of Acinetobacter spp. isolated from hospitalized patients.Methods: Sixty isolates were identified as Acinetobacter species. The isolates were tested for antibiotic resistance by disc diffusion method for 12 antimicrobials. The presence of aphA6, aacC1 aadA1, and aadB genes were detected using PCR.Results: From the isolated Acinetobacter spp. the highest resistance rate showed against amikacin, tobramycin, and ceftazidim, respectively; while isolated bacteria were more sensitive to ampicillic/subactam. More than 66% of the isolates were resistant to at least three classes of antibiotics, and 27.5% of MDR strains were resistant to all seven tested classes of antimicrobials. The higher MDR rate presented in bacteria isolated from the ICU and blood samples. More than 60% of the MDR bacteria were resistance to amikacin, ceftazidim, ciprofloxacin, piperacillin/tazobactam, doxycycline, tobramycin and levofloxacin. Also, more than 60% of the isolates contained phosphotransferase aphA6, and acetyltransferase genes aacC1, but adenylyltransferase genes aadA1 (41.7%), and aadB (3.3%) were less prominent. 21.7% of the strains contain three aminoglycoside resistance genes (aphA6, aacC1 and aadA1).Conclusion: The rising trend of resistance to aminoglycosides poses an alarming threat to treatment of such infections. The findings showed that clinical isolates of Acinetobacter spp. in our hospital carrying various kinds of aminoglycoside resistance genes.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/15622.pdf&manuscript_id=15622Acinetobacter SppAntibiotic ResistancePCR Gene DetectionAminoglycoside Resistance GenesIran |
| spellingShingle | MH Nazem Shirazi Gh Shajari R Kheltabadi Farahani R Moniri A Ghasemi Molecular Epidemiology of Aminoglycosides Resistance in Acinetobacter Spp. with Emergence of Multidrug-Resistant Strains Iranian Journal of Public Health Acinetobacter Spp Antibiotic Resistance PCR Gene Detection Aminoglycoside Resistance Genes Iran |
| title | Molecular Epidemiology of Aminoglycosides Resistance in Acinetobacter Spp. with Emergence of Multidrug-Resistant Strains |
| title_full | Molecular Epidemiology of Aminoglycosides Resistance in Acinetobacter Spp. with Emergence of Multidrug-Resistant Strains |
| title_fullStr | Molecular Epidemiology of Aminoglycosides Resistance in Acinetobacter Spp. with Emergence of Multidrug-Resistant Strains |
| title_full_unstemmed | Molecular Epidemiology of Aminoglycosides Resistance in Acinetobacter Spp. with Emergence of Multidrug-Resistant Strains |
| title_short | Molecular Epidemiology of Aminoglycosides Resistance in Acinetobacter Spp. with Emergence of Multidrug-Resistant Strains |
| title_sort | molecular epidemiology of aminoglycosides resistance in acinetobacter spp with emergence of multidrug resistant strains |
| topic | Acinetobacter Spp Antibiotic Resistance PCR Gene Detection Aminoglycoside Resistance Genes Iran |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/15622.pdf&manuscript_id=15622 |
| work_keys_str_mv | AT mhnazemshirazi molecularepidemiologyofaminoglycosidesresistanceinacinetobactersppwithemergenceofmultidrugresistantstrains AT ghshajari molecularepidemiologyofaminoglycosidesresistanceinacinetobactersppwithemergenceofmultidrugresistantstrains AT rkheltabadifarahani molecularepidemiologyofaminoglycosidesresistanceinacinetobactersppwithemergenceofmultidrugresistantstrains AT rmoniri molecularepidemiologyofaminoglycosidesresistanceinacinetobactersppwithemergenceofmultidrugresistantstrains AT aghasemi molecularepidemiologyofaminoglycosidesresistanceinacinetobactersppwithemergenceofmultidrugresistantstrains |