Phytochemical screening and GC-MS chemical profiling of an innovative anti-cancer herbal formula (PHF6)
The present investigation assessed the anticancer potential and chemical profile of the polyherbal formulation (PHF6) against human breast cancer cells. The plants (Alchemilla vulgaris, Cichorium pumilum, Crataegus azarolus, Eruca sativa, Ferula hermonis, and Hypericum triquetrifolium) were combined...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722007066 |
| _version_ | 1797952837280333824 |
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| author | Mohammed Al-Zharani Nael Abutaha |
| author_facet | Mohammed Al-Zharani Nael Abutaha |
| author_sort | Mohammed Al-Zharani |
| collection | DOAJ |
| description | The present investigation assessed the anticancer potential and chemical profile of the polyherbal formulation (PHF6) against human breast cancer cells. The plants (Alchemilla vulgaris, Cichorium pumilum, Crataegus azarolus, Eruca sativa, Ferula hermonis, and Hypericum triquetrifolium) were combined in equal quantities and extracted using soxhlet apparatus. The total polyphenolic (TPC) of the hexane and chloroform extract of PHF6 were 3.11 ± 0.004 and 7.88 ± 0.004 µg/mL (as gallic acid equivalent), respectively. The radical scavenging activity (RSA) values for the hexane and chloroform extract were 35.1 % ±0.01 and 36.9 % ±0.06, respectively. For hexane extract, the IC50 values were found to be 82.8 and 48.7 µg/mL against MCF-7ER(+)/PR(+) and MDA MB-231ER(−)/PR(−), respectively. Similarly, the IC50 value of chloroform extract was 44.4 µg/mL against both cell lines. The MDA MB-231ER(−)/PR(−) cells stained with DAPI post-treatment with hexane and chloroform extract showed cell shrinkage, nuclear fragmentation, and chromatin condensation. Moreover, GC–MS screening of the PHF6 revealed several compounds with different reported biological activities, such as phytol and octadecanoic acid. PHF6 showed promising anticancer activity against cancer cells tested, thus deserving more clinical studies in the future. |
| first_indexed | 2024-04-10T22:52:19Z |
| format | Article |
| id | doaj.art-358c87b35a4c469b8bbff421ae5d7214 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-10T22:52:19Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-358c87b35a4c469b8bbff421ae5d72142023-01-15T04:21:26ZengElsevierJournal of King Saud University: Science1018-36472023-02-01352102525Phytochemical screening and GC-MS chemical profiling of an innovative anti-cancer herbal formula (PHF6)Mohammed Al-Zharani0Nael Abutaha1Imam Mohammad Ibn Saud Islamic University (IMSIU), College of Science, Biology Department, Riyadh, Saudi Arabia; Corresponding author.Bioproducts Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaThe present investigation assessed the anticancer potential and chemical profile of the polyherbal formulation (PHF6) against human breast cancer cells. The plants (Alchemilla vulgaris, Cichorium pumilum, Crataegus azarolus, Eruca sativa, Ferula hermonis, and Hypericum triquetrifolium) were combined in equal quantities and extracted using soxhlet apparatus. The total polyphenolic (TPC) of the hexane and chloroform extract of PHF6 were 3.11 ± 0.004 and 7.88 ± 0.004 µg/mL (as gallic acid equivalent), respectively. The radical scavenging activity (RSA) values for the hexane and chloroform extract were 35.1 % ±0.01 and 36.9 % ±0.06, respectively. For hexane extract, the IC50 values were found to be 82.8 and 48.7 µg/mL against MCF-7ER(+)/PR(+) and MDA MB-231ER(−)/PR(−), respectively. Similarly, the IC50 value of chloroform extract was 44.4 µg/mL against both cell lines. The MDA MB-231ER(−)/PR(−) cells stained with DAPI post-treatment with hexane and chloroform extract showed cell shrinkage, nuclear fragmentation, and chromatin condensation. Moreover, GC–MS screening of the PHF6 revealed several compounds with different reported biological activities, such as phytol and octadecanoic acid. PHF6 showed promising anticancer activity against cancer cells tested, thus deserving more clinical studies in the future.http://www.sciencedirect.com/science/article/pii/S1018364722007066AntioxidantApoptosisGC-MSPolyherbal formulation |
| spellingShingle | Mohammed Al-Zharani Nael Abutaha Phytochemical screening and GC-MS chemical profiling of an innovative anti-cancer herbal formula (PHF6) Journal of King Saud University: Science Antioxidant Apoptosis GC-MS Polyherbal formulation |
| title | Phytochemical screening and GC-MS chemical profiling of an innovative anti-cancer herbal formula (PHF6) |
| title_full | Phytochemical screening and GC-MS chemical profiling of an innovative anti-cancer herbal formula (PHF6) |
| title_fullStr | Phytochemical screening and GC-MS chemical profiling of an innovative anti-cancer herbal formula (PHF6) |
| title_full_unstemmed | Phytochemical screening and GC-MS chemical profiling of an innovative anti-cancer herbal formula (PHF6) |
| title_short | Phytochemical screening and GC-MS chemical profiling of an innovative anti-cancer herbal formula (PHF6) |
| title_sort | phytochemical screening and gc ms chemical profiling of an innovative anti cancer herbal formula phf6 |
| topic | Antioxidant Apoptosis GC-MS Polyherbal formulation |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722007066 |
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