The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam]
IbMYB1 was one of the major anthocyanin biosynthesis regulatory genes that has been identified and utilized in purple-fleshed sweet potato breeding. At least three members of this gene, namely, IbMYB1-1, -2a, and -2b, have been reported. We found that IbMYB1-2a and -2b are not necessary for anthocya...
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.688707/full |
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| author | Daowei Zhang Daowei Zhang Yongjun Tan Fang Dong Ya Zhang Yanlan Huang Yizhou Zhou ZhiJian Zhao Qin Yin Xuehua Xie Xiewang Gao Chaofan Zhang Naimei Tu |
| author_facet | Daowei Zhang Daowei Zhang Yongjun Tan Fang Dong Ya Zhang Yanlan Huang Yizhou Zhou ZhiJian Zhao Qin Yin Xuehua Xie Xiewang Gao Chaofan Zhang Naimei Tu |
| author_sort | Daowei Zhang |
| collection | DOAJ |
| description | IbMYB1 was one of the major anthocyanin biosynthesis regulatory genes that has been identified and utilized in purple-fleshed sweet potato breeding. At least three members of this gene, namely, IbMYB1-1, -2a, and -2b, have been reported. We found that IbMYB1-2a and -2b are not necessary for anthocyanin accumulation in a variety of cultivated species (hexaploid) with purple shoots or purplish rings/spots of flesh. Transcriptomic and quantitative reverse transcription PCR (RT-qPCR) analyses revealed that persistent and vigorous expression of IbMYB1 is essential to maintain the purple color of leaves and storage roots in this type of cultivated species, which did not contain IbMYB1-2 gene members. Compared with IbbHLH2, IbMYB1 is an early response gene of anthocyanin biosynthesis in sweet potato. It cannot exclude the possibility that other MYBs participate in this gene regulation networks. Twenty-two MYB-like genes were identified from 156 MYBs to be highly positively or negatively correlated with the anthocyanin content in leaves or flesh. Even so, the IbMYB1 was most coordinately expressed with anthocyanin biosynthesis genes. Differences in flanking and coding sequences confirm that IbMYB2s, the highest similarity genes of IbMYB1, are not the members of IbMYB1. This phenomenon indicates that there may be more members of IbMYB1 in sweet potato, and the genetic complementation of these members is involved in the regulation of anthocyanin biosynthesis. The 3′ flanking sequence of IbMYB1-1 is homologous to the retrotransposon sequence of TNT1-94. Transposon movement is involved in the formation of multiple members of IbMYB1. This study provides critical insights into the expression patterns of IbMYB1, which are involved in the regulation of anthocyanin biosynthesis in the leaf and storage root. Notably, our study also emphasized the presence of a multiple member of IbMYB1 for genetic improvement. |
| first_indexed | 2024-12-19T23:20:06Z |
| format | Article |
| id | doaj.art-358db99797ea42649476e71c22dd7cb7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T23:20:06Z |
| publishDate | 2021-09-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-358db99797ea42649476e71c22dd7cb72022-12-21T20:02:00ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-09-011210.3389/fpls.2021.688707688707The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam]Daowei Zhang0Daowei Zhang1Yongjun Tan2Fang Dong3Ya Zhang4Yanlan Huang5Yizhou Zhou6ZhiJian Zhao7Qin Yin8Xuehua Xie9Xiewang Gao10Chaofan Zhang11Naimei Tu12College of Agronomy, Hunan Agricultural University, Changsha, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaDepartment of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaDryland Crop Research Institute, Shao Yang Academy of Agriculture Science, Shaoyang, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaCrop Research Institute, Hunan Academy of Agricultural Sciences, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaIbMYB1 was one of the major anthocyanin biosynthesis regulatory genes that has been identified and utilized in purple-fleshed sweet potato breeding. At least three members of this gene, namely, IbMYB1-1, -2a, and -2b, have been reported. We found that IbMYB1-2a and -2b are not necessary for anthocyanin accumulation in a variety of cultivated species (hexaploid) with purple shoots or purplish rings/spots of flesh. Transcriptomic and quantitative reverse transcription PCR (RT-qPCR) analyses revealed that persistent and vigorous expression of IbMYB1 is essential to maintain the purple color of leaves and storage roots in this type of cultivated species, which did not contain IbMYB1-2 gene members. Compared with IbbHLH2, IbMYB1 is an early response gene of anthocyanin biosynthesis in sweet potato. It cannot exclude the possibility that other MYBs participate in this gene regulation networks. Twenty-two MYB-like genes were identified from 156 MYBs to be highly positively or negatively correlated with the anthocyanin content in leaves or flesh. Even so, the IbMYB1 was most coordinately expressed with anthocyanin biosynthesis genes. Differences in flanking and coding sequences confirm that IbMYB2s, the highest similarity genes of IbMYB1, are not the members of IbMYB1. This phenomenon indicates that there may be more members of IbMYB1 in sweet potato, and the genetic complementation of these members is involved in the regulation of anthocyanin biosynthesis. The 3′ flanking sequence of IbMYB1-1 is homologous to the retrotransposon sequence of TNT1-94. Transposon movement is involved in the formation of multiple members of IbMYB1. This study provides critical insights into the expression patterns of IbMYB1, which are involved in the regulation of anthocyanin biosynthesis in the leaf and storage root. Notably, our study also emphasized the presence of a multiple member of IbMYB1 for genetic improvement.https://www.frontiersin.org/articles/10.3389/fpls.2021.688707/fullpurple-fleshed sweetpotatoanthocyanin biosynthesisR2R3-MYBtranscriptomemultiple membersearly response gene |
| spellingShingle | Daowei Zhang Daowei Zhang Yongjun Tan Fang Dong Ya Zhang Yanlan Huang Yizhou Zhou ZhiJian Zhao Qin Yin Xuehua Xie Xiewang Gao Chaofan Zhang Naimei Tu The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam] Frontiers in Plant Science purple-fleshed sweetpotato anthocyanin biosynthesis R2R3-MYB transcriptome multiple members early response gene |
| title | The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam] |
| title_full | The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam] |
| title_fullStr | The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam] |
| title_full_unstemmed | The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam] |
| title_short | The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam] |
| title_sort | expression of ibmyb1 is essential to maintain the purple color of leaf and storage root in sweet potato ipomoea batatas l lam |
| topic | purple-fleshed sweetpotato anthocyanin biosynthesis R2R3-MYB transcriptome multiple members early response gene |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.688707/full |
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