Blocking Effect of Chaga Mushroom (<i>Inonotus oliquus</i>) Extract for Immune Checkpoint CTLA-4/CD80 Interaction
<i>Inonotus obliquus</i>, also known as the Chaga mushroom, has been used as a traditional medicine to treat many different diseases in Asia. Ethanol and water extraction were performed to examine the blocking effect of the Chaga mushroom on the CTLA-4/CD80 interaction. The inhibitory ac...
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MDPI AG
2020-08-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/17/5774 |
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| author | Tae In Kim Jang-Gi Choi Ji Hye Kim Wei Li Hwan-Suck Chung |
| author_facet | Tae In Kim Jang-Gi Choi Ji Hye Kim Wei Li Hwan-Suck Chung |
| author_sort | Tae In Kim |
| collection | DOAJ |
| description | <i>Inonotus obliquus</i>, also known as the Chaga mushroom, has been used as a traditional medicine to treat many different diseases in Asia. Ethanol and water extraction were performed to examine the blocking effect of the Chaga mushroom on the CTLA-4/CD80 interaction. The inhibitory activities of the Chaga mushroom/70% EtOH extract (CME) and the Chaga mushroom/water extract (CMW) were confirmed using several cell-based assays. To identify the contents of major compounds CME and CMW, we performed HPLC analysis. The content of lanosterol (<b>1</b>) in CME was 0.41%. Our findings provide experimental evidence that the Chaga mushroom can develop a small-molecule inhibitor that blocks the CTLA-4/CD80 interaction. |
| first_indexed | 2024-03-10T17:06:41Z |
| format | Article |
| id | doaj.art-35910d30322b40b4aa580b9bdd8f2a64 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T17:06:41Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-35910d30322b40b4aa580b9bdd8f2a642023-11-20T10:47:57ZengMDPI AGApplied Sciences2076-34172020-08-011017577410.3390/app10175774Blocking Effect of Chaga Mushroom (<i>Inonotus oliquus</i>) Extract for Immune Checkpoint CTLA-4/CD80 InteractionTae In Kim0Jang-Gi Choi1Ji Hye Kim2Wei Li3Hwan-Suck Chung4Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, KoreaKorean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, KoreaKorean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, KoreaKorean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, KoreaKorean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, Korea<i>Inonotus obliquus</i>, also known as the Chaga mushroom, has been used as a traditional medicine to treat many different diseases in Asia. Ethanol and water extraction were performed to examine the blocking effect of the Chaga mushroom on the CTLA-4/CD80 interaction. The inhibitory activities of the Chaga mushroom/70% EtOH extract (CME) and the Chaga mushroom/water extract (CMW) were confirmed using several cell-based assays. To identify the contents of major compounds CME and CMW, we performed HPLC analysis. The content of lanosterol (<b>1</b>) in CME was 0.41%. Our findings provide experimental evidence that the Chaga mushroom can develop a small-molecule inhibitor that blocks the CTLA-4/CD80 interaction.https://www.mdpi.com/2076-3417/10/17/5774immune checkpointsCTLA-4CD80<i>Inonotus obliquus</i> |
| spellingShingle | Tae In Kim Jang-Gi Choi Ji Hye Kim Wei Li Hwan-Suck Chung Blocking Effect of Chaga Mushroom (<i>Inonotus oliquus</i>) Extract for Immune Checkpoint CTLA-4/CD80 Interaction Applied Sciences immune checkpoints CTLA-4 CD80 <i>Inonotus obliquus</i> |
| title | Blocking Effect of Chaga Mushroom (<i>Inonotus oliquus</i>) Extract for Immune Checkpoint CTLA-4/CD80 Interaction |
| title_full | Blocking Effect of Chaga Mushroom (<i>Inonotus oliquus</i>) Extract for Immune Checkpoint CTLA-4/CD80 Interaction |
| title_fullStr | Blocking Effect of Chaga Mushroom (<i>Inonotus oliquus</i>) Extract for Immune Checkpoint CTLA-4/CD80 Interaction |
| title_full_unstemmed | Blocking Effect of Chaga Mushroom (<i>Inonotus oliquus</i>) Extract for Immune Checkpoint CTLA-4/CD80 Interaction |
| title_short | Blocking Effect of Chaga Mushroom (<i>Inonotus oliquus</i>) Extract for Immune Checkpoint CTLA-4/CD80 Interaction |
| title_sort | blocking effect of chaga mushroom i inonotus oliquus i extract for immune checkpoint ctla 4 cd80 interaction |
| topic | immune checkpoints CTLA-4 CD80 <i>Inonotus obliquus</i> |
| url | https://www.mdpi.com/2076-3417/10/17/5774 |
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