Practical Approaches to Improve the Sensitivity of the Volume-Based Cellular Enumeration of the BeWo Cell Line-Derived Spheroids as an In Vitro Embryo Model: MTT Versus Neutral Red Uptake Assays

Practical Approaches to Improve the Sensitivity of the Volume-Based Cellular Enumeration of the BeWo Cell Line-Derived Spheroids as an In Vitro Embryo Model: MTT Versus Neutral Red Uptake Assays

Objectives: Choriocarcinoma spheroids, as acceptable three-dimensional (3D) models of the embryo, vary in the number of incorporated cells based on the procedures of production. Since the secretion of emitted signals from spheroids can modulate endometrial confrontation responses, improving a high t...

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Bibliographic Details
Main Authors: Mahsa Kazemi, Vahid Jajarmi, Hamid Nazarian, Marefat Ghaffari Novin, Saghar Salehpour, Hamid Choobineh, Zahra Shams Mofarahe, Mohammad Hasan Heidari
Format: Article
Language:English
Published: Aras Part Medical International Press 2020-04-01
Series:Crescent Journal of Medical and Biological Sciences
Subjects:
cellular spheroids
choriocarcinoma
neutral red
mtt formazan
embryo implantation
Online Access:http://www.cjmb.org/pdf.php?id=408

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