Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 Cells
Purpose: Accumulating evidence indicates that glycyrrhizin (GZ) and its hydrolyzed metabolite 18-β glycyrrhetinic acid (GA) exhibit anti-inflammatory and anticancer activities. The objective of this study was to examine the in vitro cytotoxic activity of GA on human ovarian cancer A2780 cells. Meth...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2014-10-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol4NoSupp1/4-Supp1-Jalili.pdf |
| _version_ | 1819177965320142848 |
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| author | Venus Haghshenas Shohreh Fakhari Sako Mirzaie Mohammadreza Rahmani Fariba Farhadifar Sara Pirzadeh Ali Jalili |
| author_facet | Venus Haghshenas Shohreh Fakhari Sako Mirzaie Mohammadreza Rahmani Fariba Farhadifar Sara Pirzadeh Ali Jalili |
| author_sort | Venus Haghshenas |
| collection | DOAJ |
| description | Purpose: Accumulating evidence indicates that glycyrrhizin (GZ) and its hydrolyzed metabolite 18-β glycyrrhetinic acid (GA) exhibit anti-inflammatory and anticancer activities. The objective of this study was to examine the in vitro cytotoxic activity of GA on human ovarian cancer A2780 cells.
Methods: A2780 cells were cultured in RPMI1640 containing 10% fetal bovine serum. Cells were treated with different doses of GA and cell viability and proliferation were detected by dye exclusion and 3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assays. Apoptosis induction and expression of Fas and Fas ligand (FasL) were analyzed by flow cytometry.
Results: We observed that GA decreases cell viability and suppressed cells proliferation in a dose-dependent manner as detected by dye-exclusion and XTT assayes. In addition, our flow cytometry data show that GA not only induces apoptosis in A2780 cells but also upregulates both Fas and FasL on these cells in a dose-dependent manner.
Conclusion: we demonstrate that GA causes cell death in A2780 cells by inducing apoptosis. |
| first_indexed | 2024-12-22T21:35:02Z |
| format | Article |
| id | doaj.art-3593747cb09143d48a8bd326bfd37ddd |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-22T21:35:02Z |
| publishDate | 2014-10-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-3593747cb09143d48a8bd326bfd37ddd2022-12-21T18:11:47ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082014-10-014suppl 143744110.5681/apb.2014.064Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 CellsVenus Haghshenas0Shohreh Fakhari1Sako Mirzaie2Mohammadreza Rahmani3Fariba Farhadifar4Sara Pirzadeh5Ali Jalili6Department of Biochemistry, Research and Science, Islamic Azad University, Sanandaj, Iran.Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.Department of Biochemistry, Sanandaj Branch, Islamic Azad University, Iran.Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.Department of Gynecology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.Department of Biochemistry, Research and Science, Islamic Azad University, Sanandaj, Iran.Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.Purpose: Accumulating evidence indicates that glycyrrhizin (GZ) and its hydrolyzed metabolite 18-β glycyrrhetinic acid (GA) exhibit anti-inflammatory and anticancer activities. The objective of this study was to examine the in vitro cytotoxic activity of GA on human ovarian cancer A2780 cells. Methods: A2780 cells were cultured in RPMI1640 containing 10% fetal bovine serum. Cells were treated with different doses of GA and cell viability and proliferation were detected by dye exclusion and 3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assays. Apoptosis induction and expression of Fas and Fas ligand (FasL) were analyzed by flow cytometry. Results: We observed that GA decreases cell viability and suppressed cells proliferation in a dose-dependent manner as detected by dye-exclusion and XTT assayes. In addition, our flow cytometry data show that GA not only induces apoptosis in A2780 cells but also upregulates both Fas and FasL on these cells in a dose-dependent manner. Conclusion: we demonstrate that GA causes cell death in A2780 cells by inducing apoptosis.http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol4NoSupp1/4-Supp1-Jalili.pdfGlycyrrhetinic acidApoptosisOvarian cancers |
| spellingShingle | Venus Haghshenas Shohreh Fakhari Sako Mirzaie Mohammadreza Rahmani Fariba Farhadifar Sara Pirzadeh Ali Jalili Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 Cells Advanced Pharmaceutical Bulletin Glycyrrhetinic acid Apoptosis Ovarian cancers |
| title | Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 Cells |
| title_full | Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 Cells |
| title_fullStr | Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 Cells |
| title_full_unstemmed | Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 Cells |
| title_short | Glycyrrhetinic Acid Inhibits Cell Growth and Induces Apoptosis in Ovarian Cancer A2780 Cells |
| title_sort | glycyrrhetinic acid inhibits cell growth and induces apoptosis in ovarian cancer a2780 cells |
| topic | Glycyrrhetinic acid Apoptosis Ovarian cancers |
| url | http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol4NoSupp1/4-Supp1-Jalili.pdf |
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