Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis
The diagnosis of mucocutaneous leishmaniasis (MCL) is hampered by the absence of a gold standard. An accurate diagnosis is essential because of the high toxicity of the medications for the disease. This study aimed to assess the ability of polymerase chain reaction (PCR) to identify MCL and to compa...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2015-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200157&lng=en&tlng=en |
| _version_ | 1797756763101986816 |
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| author | Ciro Martins Gomes Suleimy Cristina Mazin Elisa Raphael dos Santos Mariana Vicente Cesetti Guilherme Albergaria Brízida Bächtold João Henrique de Freitas Cordeiro Fabrício Claudino Estrela Terra Theodoro Fabiana dos Santos Damasco Sebastián Andrés Vernal Carranza Adriana de Oliveira Santos Ana Maria Roselino Raimunda Nonata Ribeiro Sampaio |
| author_facet | Ciro Martins Gomes Suleimy Cristina Mazin Elisa Raphael dos Santos Mariana Vicente Cesetti Guilherme Albergaria Brízida Bächtold João Henrique de Freitas Cordeiro Fabrício Claudino Estrela Terra Theodoro Fabiana dos Santos Damasco Sebastián Andrés Vernal Carranza Adriana de Oliveira Santos Ana Maria Roselino Raimunda Nonata Ribeiro Sampaio |
| author_sort | Ciro Martins Gomes |
| collection | DOAJ |
| description | The diagnosis of mucocutaneous leishmaniasis (MCL) is hampered by the absence of a gold standard. An accurate diagnosis is essential because of the high toxicity of the medications for the disease. This study aimed to assess the ability of polymerase chain reaction (PCR) to identify MCL and to compare these results with clinical research recently published by the authors. A systematic literature review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses: the PRISMA Statement was performed using comprehensive search criteria and communication with the authors. A meta-analysis considering the estimates of the univariate and bivariate models was performed. Specificity near 100% was common among the papers. The primary reason for accuracy differences was sensitivity. The meta-analysis, which was only possible for PCR samples of lesion fragments, revealed a sensitivity of 71% [95% confidence interval (CI) = 0.59; 0.81] and a specificity of 93% (95% CI = 0.83; 0.98) in the bivariate model. The search for measures that could increase the sensitivity of PCR should be encouraged. The quality of the collected material and the optimisation of the amplification of genetic material should be prioritised. |
| first_indexed | 2024-03-12T18:06:14Z |
| format | Article |
| id | doaj.art-359466925cba46d7b6d3f476b1400203 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:06:14Z |
| publishDate | 2015-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-359466925cba46d7b6d3f476b14002032023-08-02T09:28:14ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-04-01110215716510.1590/0074-02760140280S0074-02762015000200157Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysisCiro Martins GomesSuleimy Cristina MazinElisa Raphael dos SantosMariana Vicente CesettiGuilherme Albergaria Brízida BächtoldJoão Henrique de Freitas CordeiroFabrício Claudino Estrela Terra TheodoroFabiana dos Santos DamascoSebastián Andrés Vernal CarranzaAdriana de Oliveira SantosAna Maria RoselinoRaimunda Nonata Ribeiro SampaioThe diagnosis of mucocutaneous leishmaniasis (MCL) is hampered by the absence of a gold standard. An accurate diagnosis is essential because of the high toxicity of the medications for the disease. This study aimed to assess the ability of polymerase chain reaction (PCR) to identify MCL and to compare these results with clinical research recently published by the authors. A systematic literature review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses: the PRISMA Statement was performed using comprehensive search criteria and communication with the authors. A meta-analysis considering the estimates of the univariate and bivariate models was performed. Specificity near 100% was common among the papers. The primary reason for accuracy differences was sensitivity. The meta-analysis, which was only possible for PCR samples of lesion fragments, revealed a sensitivity of 71% [95% confidence interval (CI) = 0.59; 0.81] and a specificity of 93% (95% CI = 0.83; 0.98) in the bivariate model. The search for measures that could increase the sensitivity of PCR should be encouraged. The quality of the collected material and the optimisation of the amplification of genetic material should be prioritised.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200157&lng=en&tlng=enleishmaniasis, mucocutaneousdiagnosispolymerase chain reaction |
| spellingShingle | Ciro Martins Gomes Suleimy Cristina Mazin Elisa Raphael dos Santos Mariana Vicente Cesetti Guilherme Albergaria Brízida Bächtold João Henrique de Freitas Cordeiro Fabrício Claudino Estrela Terra Theodoro Fabiana dos Santos Damasco Sebastián Andrés Vernal Carranza Adriana de Oliveira Santos Ana Maria Roselino Raimunda Nonata Ribeiro Sampaio Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis Memorias do Instituto Oswaldo Cruz leishmaniasis, mucocutaneous diagnosis polymerase chain reaction |
| title | Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis |
| title_full | Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis |
| title_fullStr | Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis |
| title_full_unstemmed | Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis |
| title_short | Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis |
| title_sort | accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction systematic literature review and meta analysis |
| topic | leishmaniasis, mucocutaneous diagnosis polymerase chain reaction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200157&lng=en&tlng=en |
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