A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells.
The vigorous cytokine response of immune cells to Gram-negative bacteria is primarily mediated by a recognition molecule, Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide (LPS) and initiates a series of intracellular NF-kappaB-associated signaling events. Recently, bladder epithelial...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-04-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.0030060 |
| _version_ | 1829502699579637760 |
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| author | Jeongmin Song Matthew J Duncan Guojie Li Cheryl Chan Richard Grady Ann Stapleton Soman N Abraham |
| author_facet | Jeongmin Song Matthew J Duncan Guojie Li Cheryl Chan Richard Grady Ann Stapleton Soman N Abraham |
| author_sort | Jeongmin Song |
| collection | DOAJ |
| description | The vigorous cytokine response of immune cells to Gram-negative bacteria is primarily mediated by a recognition molecule, Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide (LPS) and initiates a series of intracellular NF-kappaB-associated signaling events. Recently, bladder epithelial cells (BECs) were reported to express TLR4 and to evoke a vigorous cytokine response upon exposure to LPS. We examined intracellular signaling events in human BECs leading to the production of IL-6, a major urinary cytokine, following activation by Escherichia coli and isolated LPS. We observed that in addition to the classical NF-kappaB-associated pathway, TLR4 triggers a distinct and more rapid signaling response involving, sequentially, Ca(2+), adenylyl cyclase 3-generated cAMP, and a transcriptional factor, cAMP response element-binding protein. This capacity of BECs to mobilize secondary messengers and evoke a more rapid IL-6 response might be critical in their role as first responders to microbial challenge in the urinary tract. |
| first_indexed | 2024-12-16T09:42:11Z |
| format | Article |
| id | doaj.art-3595cf58b28e4ef9acf346d73856d29a |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-16T09:42:11Z |
| publishDate | 2007-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-3595cf58b28e4ef9acf346d73856d29a2022-12-21T22:36:14ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742007-04-0134e6010.1371/journal.ppat.0030060A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells.Jeongmin SongMatthew J DuncanGuojie LiCheryl ChanRichard GradyAnn StapletonSoman N AbrahamThe vigorous cytokine response of immune cells to Gram-negative bacteria is primarily mediated by a recognition molecule, Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide (LPS) and initiates a series of intracellular NF-kappaB-associated signaling events. Recently, bladder epithelial cells (BECs) were reported to express TLR4 and to evoke a vigorous cytokine response upon exposure to LPS. We examined intracellular signaling events in human BECs leading to the production of IL-6, a major urinary cytokine, following activation by Escherichia coli and isolated LPS. We observed that in addition to the classical NF-kappaB-associated pathway, TLR4 triggers a distinct and more rapid signaling response involving, sequentially, Ca(2+), adenylyl cyclase 3-generated cAMP, and a transcriptional factor, cAMP response element-binding protein. This capacity of BECs to mobilize secondary messengers and evoke a more rapid IL-6 response might be critical in their role as first responders to microbial challenge in the urinary tract.https://doi.org/10.1371/journal.ppat.0030060 |
| spellingShingle | Jeongmin Song Matthew J Duncan Guojie Li Cheryl Chan Richard Grady Ann Stapleton Soman N Abraham A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. PLoS Pathogens |
| title | A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. |
| title_full | A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. |
| title_fullStr | A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. |
| title_full_unstemmed | A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. |
| title_short | A novel TLR4-mediated signaling pathway leading to IL-6 responses in human bladder epithelial cells. |
| title_sort | novel tlr4 mediated signaling pathway leading to il 6 responses in human bladder epithelial cells |
| url | https://doi.org/10.1371/journal.ppat.0030060 |
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