Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure
Implant failure, which is commonly associated with failure of osseointegration and peri-implant infection, is a severe complication of orthopedic surgery. In particular, the survival rate of implants is significantly decreased in patients using long-term glucocorticoids (GCs). However, the exact mol...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2023-04-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X22003930 |
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| author | Zhe Liu Qian Tang Ruo-Tao Liu Ming-Zhao Yu Hao Peng Chang-Qing Zhang Zhen-Zhong Zhu Xiao-Juan Wei |
| author_facet | Zhe Liu Qian Tang Ruo-Tao Liu Ming-Zhao Yu Hao Peng Chang-Qing Zhang Zhen-Zhong Zhu Xiao-Juan Wei |
| author_sort | Zhe Liu |
| collection | DOAJ |
| description | Implant failure, which is commonly associated with failure of osseointegration and peri-implant infection, is a severe complication of orthopedic surgery. In particular, the survival rate of implants is significantly decreased in patients using long-term glucocorticoids (GCs). However, the exact molecular mechanism underlying GCs-induced implant loosening, as well as preventive strategies for these patients, is unclear. To address this problem, we performed RNA-sequencing and found that WNT16 was correlated with GCs-induced osteopenia (LogFC = −5.17, p ＜ 0.01). Inspired by the concept of “organic-inorganic” hybrid, we theorized to introduce a bioactive two-dimensional nanosheet into a layer-by-layer (LbL) self-assembly coating to construct a customized implant that targets WNT16. After screening commercially available nanosheets, laponite (LAP) was identified as a cost-effective rescuer for GCs-induced WNT16 inhibition, which was then intercalated into LbL deposition system consisting of quaternized chitosan (QCS) and hyaluronic acid (HA). The hybrid coating (QCS/HA/LAP) showed micrometer thickness and improved hydrophilicity and interface roughness. Furthermore, QCS/HA/LAP coated polyetheretherketone (PEEK) implant enhanced cell viability, adhesion, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and promoted osteointegration of PEEK in GCs-treated rats by targeting the WNT16/β-catenin axis. The assembled QCS has proven antibacterial properties, and the hybrid coating exerted potent detrimental effects against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli), both in vitro and in vivo. Taken together, these results suggest that QCS/HA/LAP coating has great potential for use in implants customization, and has synergistic pro-osteogenic and antibacterial effects that help prevent implant failure in GCs-treated patients. |
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| language | English |
| last_indexed | 2024-04-24T08:34:16Z |
| publishDate | 2023-04-01 |
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| spelling | doaj.art-35967bf64cbc40e982010afee67e0f952024-04-16T17:41:31ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-04-01226073Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failureZhe Liu0Qian Tang1Ruo-Tao Liu2Ming-Zhao Yu3Hao Peng4Chang-Qing Zhang5Zhen-Zhong Zhu6Xiao-Juan Wei7Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233, ChinaDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233, ChinaDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233, ChinaDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233, ChinaDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233, ChinaDepartment of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233, China; Corresponding author.Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233, China; Corresponding author.Institute of Microsurgery on Extremities, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233, China; Corresponding author.Implant failure, which is commonly associated with failure of osseointegration and peri-implant infection, is a severe complication of orthopedic surgery. In particular, the survival rate of implants is significantly decreased in patients using long-term glucocorticoids (GCs). However, the exact molecular mechanism underlying GCs-induced implant loosening, as well as preventive strategies for these patients, is unclear. To address this problem, we performed RNA-sequencing and found that WNT16 was correlated with GCs-induced osteopenia (LogFC = −5.17, p ＜ 0.01). Inspired by the concept of “organic-inorganic” hybrid, we theorized to introduce a bioactive two-dimensional nanosheet into a layer-by-layer (LbL) self-assembly coating to construct a customized implant that targets WNT16. After screening commercially available nanosheets, laponite (LAP) was identified as a cost-effective rescuer for GCs-induced WNT16 inhibition, which was then intercalated into LbL deposition system consisting of quaternized chitosan (QCS) and hyaluronic acid (HA). The hybrid coating (QCS/HA/LAP) showed micrometer thickness and improved hydrophilicity and interface roughness. Furthermore, QCS/HA/LAP coated polyetheretherketone (PEEK) implant enhanced cell viability, adhesion, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and promoted osteointegration of PEEK in GCs-treated rats by targeting the WNT16/β-catenin axis. The assembled QCS has proven antibacterial properties, and the hybrid coating exerted potent detrimental effects against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli), both in vitro and in vivo. Taken together, these results suggest that QCS/HA/LAP coating has great potential for use in implants customization, and has synergistic pro-osteogenic and antibacterial effects that help prevent implant failure in GCs-treated patients.http://www.sciencedirect.com/science/article/pii/S2452199X22003930Layer-by-layer self-assemblyOrganic-inorganic hybridGlucocorticoidOsseointegrationAntibacterial |
| spellingShingle | Zhe Liu Qian Tang Ruo-Tao Liu Ming-Zhao Yu Hao Peng Chang-Qing Zhang Zhen-Zhong Zhu Xiao-Juan Wei Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure Bioactive Materials Layer-by-layer self-assembly Organic-inorganic hybrid Glucocorticoid Osseointegration Antibacterial |
| title | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
| title_full | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
| title_fullStr | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
| title_full_unstemmed | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
| title_short | Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
| title_sort | laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure |
| topic | Layer-by-layer self-assembly Organic-inorganic hybrid Glucocorticoid Osseointegration Antibacterial |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X22003930 |
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