Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish
ABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (N...
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Academia Brasileira de Ciências
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200661&lng=en&tlng=en |
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| author | DA HYE KWON JIN WOO JEONG EUN OK CHOI HYE WON LEE KI WON LEE KI YOUNG KIM SUNG GOO KIM SU HYUN HONG GI-YOUNG KIM CHEOL PARK HYE-JIN HWANG CHANG-GUE SON YUNG HYUN CHOI |
| author_facet | DA HYE KWON JIN WOO JEONG EUN OK CHOI HYE WON LEE KI WON LEE KI YOUNG KIM SUNG GOO KIM SU HYUN HONG GI-YOUNG KIM CHEOL PARK HYE-JIN HWANG CHANG-GUE SON YUNG HYUN CHOI |
| author_sort | DA HYE KWON |
| collection | DOAJ |
| description | ABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS-induced intracellular ROS production in RAW 264.7 macrophages. It inhibited the nuclear translocation of the nuclear factor-kappa B p65 subunit and the activation of mitogen-activated protein kinases in LPS-treated RAW 264.7 macrophages. Furthermore, WEMF reduced LPS-induced NO production and ROS accumulation in zebrafish. Although more efforts are needed to fully understand the critical role of WEMF in the inhibition of inflammation, the findings of the present study may provide insights into the approaches for Mori folium as a potential therapeutic agent for inflammatory and antioxidant disorders. |
| first_indexed | 2024-12-21T05:40:36Z |
| format | Article |
| id | doaj.art-3597377f158a468b8ecf9b5173544782 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-21T05:40:36Z |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-3597377f158a468b8ecf9b51735447822022-12-21T19:14:17ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-2690891 suppl66167410.1590/0001-3765201720160836S0001-37652017000200661Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafishDA HYE KWONJIN WOO JEONGEUN OK CHOIHYE WON LEEKI WON LEEKI YOUNG KIMSUNG GOO KIMSU HYUN HONGGI-YOUNG KIMCHEOL PARKHYE-JIN HWANGCHANG-GUE SONYUNG HYUN CHOIABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS-induced intracellular ROS production in RAW 264.7 macrophages. It inhibited the nuclear translocation of the nuclear factor-kappa B p65 subunit and the activation of mitogen-activated protein kinases in LPS-treated RAW 264.7 macrophages. Furthermore, WEMF reduced LPS-induced NO production and ROS accumulation in zebrafish. Although more efforts are needed to fully understand the critical role of WEMF in the inhibition of inflammation, the findings of the present study may provide insights into the approaches for Mori folium as a potential therapeutic agent for inflammatory and antioxidant disorders.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200661&lng=en&tlng=enMori foliuminflammationROSmacrophagezebrafish |
| spellingShingle | DA HYE KWON JIN WOO JEONG EUN OK CHOI HYE WON LEE KI WON LEE KI YOUNG KIM SUNG GOO KIM SU HYUN HONG GI-YOUNG KIM CHEOL PARK HYE-JIN HWANG CHANG-GUE SON YUNG HYUN CHOI Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish Anais da Academia Brasileira de Ciências Mori folium inflammation ROS macrophage zebrafish |
| title | Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish |
| title_full | Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish |
| title_fullStr | Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish |
| title_full_unstemmed | Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish |
| title_short | Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish |
| title_sort | inhibitory effects on the production of inflammatory mediators and reactive oxygen species by mori folium in lipopolysaccharide stimulated macrophages and zebrafish |
| topic | Mori folium inflammation ROS macrophage zebrafish |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200661&lng=en&tlng=en |
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