Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications
Yan Xia,1,* Panyu Zhou,1,* Xiaosong Cheng,1,* Yang Xie,1,* Chong Liang,2 Chao Li,1 Shuogui Xu1,2 1Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 2Department of Neurosurgery, The 81 Hospital of People's Liberatio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2013-11-01
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| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/selective-laser-sintering-fabrication-of-nano-hydroxyapatitepoly-epsil-a14873 |
| _version_ | 1818842259232129024 |
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| author | Xia Y Zhou PY Cheng XS Xie Y Liang C Li C Xu SG |
| author_facet | Xia Y Zhou PY Cheng XS Xie Y Liang C Li C Xu SG |
| author_sort | Xia Y |
| collection | DOAJ |
| description | Yan Xia,1,* Panyu Zhou,1,* Xiaosong Cheng,1,* Yang Xie,1,* Chong Liang,2 Chao Li,1 Shuogui Xu1,2 1Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 2Department of Neurosurgery, The 81 Hospital of People's Liberation Army of China, Nanjing, People's Republic of China *These authors contributed equally to this work Abstract: The regeneration of functional tissue in osseous defects is a formidable challenge in orthopedic surgery. In the present study, a novel biomimetic composite scaffold, here called nano-hydroxyapatite (HA)/poly-ε-caprolactone (PCL) was fabricated using a selective laser sintering technique. The macrostructure, morphology, and mechanical strength of the scaffolds were characterized. Scanning electronic microscopy (SEM) showed that the nano-HA/PCL scaffolds exhibited predesigned, well-ordered macropores and interconnected micropores. The scaffolds have a range of porosity from 78.54% to 70.31%, and a corresponding compressive strength of 1.38 MPa to 3.17 MPa. Human bone marrow stromal cells were seeded onto the nano-HA/PCL or PCL scaffolds and cultured for 28 days in vitro. As indicated by the level of cell attachment and proliferation, the nano-HA/PCL showed excellent biocompatibility, comparable to that of PCL scaffolds. The hydrophilicity, mineralization, alkaline phosphatase activity, and Alizarin Red S staining indicated that the nano-HA/PCL scaffolds are more bioactive than the PCL scaffolds in vitro. Measurements of recombinant human bone morphogenetic protein-2 (rhBMP-2) release kinetics showed that after nano-HA was added, the material increased the rate of rhBMP-2 release. To investigate the in vivo biocompatibility and osteogenesis of the composite scaffolds, both nano-HA/PCL scaffolds and PCL scaffolds were implanted in rabbit femur defects for 3, 6, and 9 weeks. The wounds were studied radiographically and histologically. The in vivo results showed that both nano-HA/PCL composite scaffolds and PCL scaffolds exhibited good biocompatibility. However, the nano-HA/PCL scaffolds enhanced the efficiency of new bone formation more than PCL scaffolds and fulfilled all the basic requirements of bone tissue engineering scaffolds. Thus, they show large potential for use in orthopedic and reconstructive surgery. Keywords: osseous defects, orthopedic surgery, biomimetic composite scaffold, reconstructive surgery |
| first_indexed | 2024-12-19T04:39:08Z |
| format | Article |
| id | doaj.art-359c215f117c4c30bb8830032a4bb8ed |
| institution | Directory Open Access Journal |
| issn | 1176-9114 1178-2013 |
| language | English |
| last_indexed | 2024-12-19T04:39:08Z |
| publishDate | 2013-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-359c215f117c4c30bb8830032a4bb8ed2022-12-21T20:35:41ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132013-11-012013Issue 141974213Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applicationsXia YZhou PYCheng XSXie YLiang CLi CXu SGYan Xia,1,* Panyu Zhou,1,* Xiaosong Cheng,1,* Yang Xie,1,* Chong Liang,2 Chao Li,1 Shuogui Xu1,2 1Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 2Department of Neurosurgery, The 81 Hospital of People's Liberation Army of China, Nanjing, People's Republic of China *These authors contributed equally to this work Abstract: The regeneration of functional tissue in osseous defects is a formidable challenge in orthopedic surgery. In the present study, a novel biomimetic composite scaffold, here called nano-hydroxyapatite (HA)/poly-ε-caprolactone (PCL) was fabricated using a selective laser sintering technique. The macrostructure, morphology, and mechanical strength of the scaffolds were characterized. Scanning electronic microscopy (SEM) showed that the nano-HA/PCL scaffolds exhibited predesigned, well-ordered macropores and interconnected micropores. The scaffolds have a range of porosity from 78.54% to 70.31%, and a corresponding compressive strength of 1.38 MPa to 3.17 MPa. Human bone marrow stromal cells were seeded onto the nano-HA/PCL or PCL scaffolds and cultured for 28 days in vitro. As indicated by the level of cell attachment and proliferation, the nano-HA/PCL showed excellent biocompatibility, comparable to that of PCL scaffolds. The hydrophilicity, mineralization, alkaline phosphatase activity, and Alizarin Red S staining indicated that the nano-HA/PCL scaffolds are more bioactive than the PCL scaffolds in vitro. Measurements of recombinant human bone morphogenetic protein-2 (rhBMP-2) release kinetics showed that after nano-HA was added, the material increased the rate of rhBMP-2 release. To investigate the in vivo biocompatibility and osteogenesis of the composite scaffolds, both nano-HA/PCL scaffolds and PCL scaffolds were implanted in rabbit femur defects for 3, 6, and 9 weeks. The wounds were studied radiographically and histologically. The in vivo results showed that both nano-HA/PCL composite scaffolds and PCL scaffolds exhibited good biocompatibility. However, the nano-HA/PCL scaffolds enhanced the efficiency of new bone formation more than PCL scaffolds and fulfilled all the basic requirements of bone tissue engineering scaffolds. Thus, they show large potential for use in orthopedic and reconstructive surgery. Keywords: osseous defects, orthopedic surgery, biomimetic composite scaffold, reconstructive surgeryhttp://www.dovepress.com/selective-laser-sintering-fabrication-of-nano-hydroxyapatitepoly-epsil-a14873 |
| spellingShingle | Xia Y Zhou PY Cheng XS Xie Y Liang C Li C Xu SG Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications International Journal of Nanomedicine |
| title | Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications |
| title_full | Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications |
| title_fullStr | Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications |
| title_full_unstemmed | Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications |
| title_short | Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications |
| title_sort | selective laser sintering fabrication of nano hydroxyapatite poly epsilon caprolactone scaffolds for bone tissue engineering applications |
| url | http://www.dovepress.com/selective-laser-sintering-fabrication-of-nano-hydroxyapatitepoly-epsil-a14873 |
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