Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)
In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5)...
| Format: | Article |
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| Language: | English |
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Iranian Medicinal Plants Society
2014-02-01
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| Series: | Journal of Medicinal Plants and By-products |
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| Online Access: | http://jmpb.areeo.ac.ir/article_108734_828fd2857a7b7b4788487da60b5d9723.pdf |
| _version_ | 1811213684369784832 |
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| collection | DOAJ |
| description | In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared. The in vitro shoots were transferred on AN media supplemented with zeatin (1 and 2 mg L-1) alone or in combination with Indole-3-butyric acid (IBA) (0.2 and 0.4 mg L-1). To optimize the rooting stage, elongated shoots (1.5- 2cm) were cultured on half strength semi- solid media containing activated charcoal (0 and 7 g L-1),or half strength liquid AN medium supplemented with different concentrations of IBA (0, 0.5, 2 and 3 mg L-1) and NAA (0 and 0.5 mg L-1). The results indicated that at the establishment stage AN media supplemented with 4 mg L-1 zeatin (pH= 5.5) and 1 mg L-1 zeatin (pH= 5) were the best treatment for Asalem and Kelardasht explants, respectively. For the Asalem explants, the highest number of proliferated shoots (4) was observed on AN media consisting of 2 mg L-1 zeatin and 0.4 mg L-1 IBA. Whereas for the Kelardash explants the highest number of proliferated shoots (3) was observed on AN media consisting of 2 mg L-1 zeatin and 0.2 mg L-1 IBA. The highest percent of root formation (86 and 66%) were observed in half strength AN medium containing of 7 g L-1 activated charcoal and 3 mg L-1 IBA and 0.5 mg L-1 NAA in Asalem and Kelardasht explants, respectively. The plantlets were successfully transferred to soil and the survival rate was 85%. |
| first_indexed | 2024-04-12T05:50:23Z |
| format | Article |
| id | doaj.art-35a2c7996b8d4e0ea087018a56261a05 |
| institution | Directory Open Access Journal |
| issn | 2322-1399 2588-3739 |
| language | English |
| last_indexed | 2024-04-12T05:50:23Z |
| publishDate | 2014-02-01 |
| publisher | Iranian Medicinal Plants Society |
| record_format | Article |
| series | Journal of Medicinal Plants and By-products |
| spelling | doaj.art-35a2c7996b8d4e0ea087018a56261a052022-12-22T03:45:19ZengIranian Medicinal Plants SocietyJournal of Medicinal Plants and By-products2322-13992588-37392014-02-0132199205108734Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared. The in vitro shoots were transferred on AN media supplemented with zeatin (1 and 2 mg L-1) alone or in combination with Indole-3-butyric acid (IBA) (0.2 and 0.4 mg L-1). To optimize the rooting stage, elongated shoots (1.5- 2cm) were cultured on half strength semi- solid media containing activated charcoal (0 and 7 g L-1),or half strength liquid AN medium supplemented with different concentrations of IBA (0, 0.5, 2 and 3 mg L-1) and NAA (0 and 0.5 mg L-1). The results indicated that at the establishment stage AN media supplemented with 4 mg L-1 zeatin (pH= 5.5) and 1 mg L-1 zeatin (pH= 5) were the best treatment for Asalem and Kelardasht explants, respectively. For the Asalem explants, the highest number of proliferated shoots (4) was observed on AN media consisting of 2 mg L-1 zeatin and 0.4 mg L-1 IBA. Whereas for the Kelardash explants the highest number of proliferated shoots (3) was observed on AN media consisting of 2 mg L-1 zeatin and 0.2 mg L-1 IBA. The highest percent of root formation (86 and 66%) were observed in half strength AN medium containing of 7 g L-1 activated charcoal and 3 mg L-1 IBA and 0.5 mg L-1 NAA in Asalem and Kelardasht explants, respectively. The plantlets were successfully transferred to soil and the survival rate was 85%.http://jmpb.areeo.ac.ir/article_108734_828fd2857a7b7b4788487da60b5d9723.pdfMedicinal plantsMicropropagationIn Vitrogrowth regulatorsVaccinium arctostaphylus |
| spellingShingle | Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus) Journal of Medicinal Plants and By-products Medicinal plants Micropropagation In Vitro growth regulators Vaccinium arctostaphylus |
| title | Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus) |
| title_full | Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus) |
| title_fullStr | Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus) |
| title_full_unstemmed | Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus) |
| title_short | Optimization of In vitro Propagation of Qare-Qat (Vaccinium arctostaphylus) |
| title_sort | optimization of in vitro propagation of qare qat vaccinium arctostaphylus |
| topic | Medicinal plants Micropropagation In Vitro growth regulators Vaccinium arctostaphylus |
| url | http://jmpb.areeo.ac.ir/article_108734_828fd2857a7b7b4788487da60b5d9723.pdf |