Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants
Arabidopsis NADK2 (NAD kinase 2) is a chloroplast-localized enzyme involved in NADP+ synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2023.2215618 |
| _version_ | 1797351954681167872 |
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| author | chaomurilege Atsuko Miyagi Toshiki Ishikawa Masatoshi Yamaguchi Hideki Murayama Maki Kawai-Yamada |
| author_facet | chaomurilege Atsuko Miyagi Toshiki Ishikawa Masatoshi Yamaguchi Hideki Murayama Maki Kawai-Yamada |
| author_sort | chaomurilege |
| collection | DOAJ |
| description | Arabidopsis NADK2 (NAD kinase 2) is a chloroplast-localized enzyme involved in NADP+ synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf senescence. During senescence, WT plants and nadk2 mutants showed a similar reduction in chlorophyll content. NAD(P)(H) quantification showed that the amount of total NAD(P)(H) decreased on the day 7 in WT but on the day 3 in nadk2. The phosphorylation ratio (i.e. NADP(H)/NAD(H)) decreased on day 1 in WT. In contrast, the nadk2 showed lower phosphorylation ratio at 0 day and no change throughout the aging process. Metabolome analysis showed that the metabolic profiles of both WT plants and nadk2 mutants subjected to dark-induced senescence adopted similar patterns as the senescence progressed. However, the changes in individual metabolites in the nadk2 mutants were different from those of the WT during dark-induced senescence. |
| first_indexed | 2024-03-08T13:08:07Z |
| format | Article |
| id | doaj.art-35a80094ae4c470eb6291d282e4f68c5 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:07Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-35a80094ae4c470eb6291d282e4f68c52024-01-18T15:58:22ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242023-12-0118110.1080/15592324.2023.22156182215618Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutantschaomurilege0Atsuko Miyagi1Toshiki Ishikawa2Masatoshi Yamaguchi3Hideki Murayama4Maki Kawai-Yamada5Saitama UniversityYamagata UniversitySaitama UniversitySaitama UniversityYamagata UniversitySaitama UniversityArabidopsis NADK2 (NAD kinase 2) is a chloroplast-localized enzyme involved in NADP+ synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf senescence. During senescence, WT plants and nadk2 mutants showed a similar reduction in chlorophyll content. NAD(P)(H) quantification showed that the amount of total NAD(P)(H) decreased on the day 7 in WT but on the day 3 in nadk2. The phosphorylation ratio (i.e. NADP(H)/NAD(H)) decreased on day 1 in WT. In contrast, the nadk2 showed lower phosphorylation ratio at 0 day and no change throughout the aging process. Metabolome analysis showed that the metabolic profiles of both WT plants and nadk2 mutants subjected to dark-induced senescence adopted similar patterns as the senescence progressed. However, the changes in individual metabolites in the nadk2 mutants were different from those of the WT during dark-induced senescence.http://dx.doi.org/10.1080/15592324.2023.2215618arabidopsis thalianace-msnad kinasenad(p)(h)metabolites |
| spellingShingle | chaomurilege Atsuko Miyagi Toshiki Ishikawa Masatoshi Yamaguchi Hideki Murayama Maki Kawai-Yamada Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants Plant Signaling & Behavior arabidopsis thaliana ce-ms nad kinase nad(p)(h) metabolites |
| title | Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants |
| title_full | Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants |
| title_fullStr | Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants |
| title_full_unstemmed | Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants |
| title_short | Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants |
| title_sort | metabolic changes associated with dark induced leaf senescence in arabidopsis nadk2 mutants |
| topic | arabidopsis thaliana ce-ms nad kinase nad(p)(h) metabolites |
| url | http://dx.doi.org/10.1080/15592324.2023.2215618 |
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