Rules of engagement: centrosome–nuclear connections in a closed mitotic system
Summary The assembly of a functional mitotic spindle is essential for cell reproduction and requires a precise coordination between the nuclear cycle and the centrosome. This coordination is particularly prominent in organisms that undergo closed mitosis where centrosomes must not only respond to te...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2012-09-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/1/11/1111 |
| _version_ | 1818677972273463296 |
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| author | Meredith Leo Diana Santino Irina Tikhonenko Valentin Magidson Alexey Khodjakov Michael P. Koonce |
| author_facet | Meredith Leo Diana Santino Irina Tikhonenko Valentin Magidson Alexey Khodjakov Michael P. Koonce |
| author_sort | Meredith Leo |
| collection | DOAJ |
| description | Summary
The assembly of a functional mitotic spindle is essential for cell reproduction and requires a precise coordination between the nuclear cycle and the centrosome. This coordination is particularly prominent in organisms that undergo closed mitosis where centrosomes must not only respond to temporal signals, but also to spatial considerations, e.g. switching from the production of cytoplasmic microtubule arrays to the generation of dynamic intra-nuclear microtubules required for spindle assembly. We utilize a gene knockout of Kif9, a Dictyostelium discoideum Kin-I kinesin, to destabilize the physical association between centrosomes and the nuclear envelope. This approach presents a unique opportunity to reveal temporal and spatial components in the regulation of centrosomal activities in a closed-mitosis organism. Here we report that centrosome–nuclear engagement is not required for the entry into mitosis. Although detached centrosomes can duplicate in the cytoplasm, neither they nor nuclei alone can produce spindle-like microtubule arrays. However, the physical association of centrosomes and the nuclear envelope is required to progress through mitosis beyond prometaphase. |
| first_indexed | 2024-12-17T09:07:51Z |
| format | Article |
| id | doaj.art-35aa41b31eab4413bfd2a1f4eba9684f |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T09:07:51Z |
| publishDate | 2012-09-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-35aa41b31eab4413bfd2a1f4eba9684f2022-12-21T21:55:23ZengThe Company of BiologistsBiology Open2046-63902012-09-011111111111710.1242/bio.2012218820122188Rules of engagement: centrosome–nuclear connections in a closed mitotic systemMeredith LeoDiana SantinoIrina TikhonenkoValentin MagidsonAlexey KhodjakovMichael P. KoonceSummary The assembly of a functional mitotic spindle is essential for cell reproduction and requires a precise coordination between the nuclear cycle and the centrosome. This coordination is particularly prominent in organisms that undergo closed mitosis where centrosomes must not only respond to temporal signals, but also to spatial considerations, e.g. switching from the production of cytoplasmic microtubule arrays to the generation of dynamic intra-nuclear microtubules required for spindle assembly. We utilize a gene knockout of Kif9, a Dictyostelium discoideum Kin-I kinesin, to destabilize the physical association between centrosomes and the nuclear envelope. This approach presents a unique opportunity to reveal temporal and spatial components in the regulation of centrosomal activities in a closed-mitosis organism. Here we report that centrosome–nuclear engagement is not required for the entry into mitosis. Although detached centrosomes can duplicate in the cytoplasm, neither they nor nuclei alone can produce spindle-like microtubule arrays. However, the physical association of centrosomes and the nuclear envelope is required to progress through mitosis beyond prometaphase.http://bio.biologists.org/content/1/11/1111CentrosomeNucleusClosed mitosisDictyostelium |
| spellingShingle | Meredith Leo Diana Santino Irina Tikhonenko Valentin Magidson Alexey Khodjakov Michael P. Koonce Rules of engagement: centrosome–nuclear connections in a closed mitotic system Biology Open Centrosome Nucleus Closed mitosis Dictyostelium |
| title | Rules of engagement: centrosome–nuclear connections in a closed mitotic system |
| title_full | Rules of engagement: centrosome–nuclear connections in a closed mitotic system |
| title_fullStr | Rules of engagement: centrosome–nuclear connections in a closed mitotic system |
| title_full_unstemmed | Rules of engagement: centrosome–nuclear connections in a closed mitotic system |
| title_short | Rules of engagement: centrosome–nuclear connections in a closed mitotic system |
| title_sort | rules of engagement centrosome nuclear connections in a closed mitotic system |
| topic | Centrosome Nucleus Closed mitosis Dictyostelium |
| url | http://bio.biologists.org/content/1/11/1111 |
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