Circadian dynamics of the teleost skin immune-microbiome interface
Abstract Background Circadian rhythms of host immune activity and their microbiomes are likely pivotal to health and disease resistance. The integration of chronotherapeutic approaches to disease mitigation in managed animals, however, is yet to be realised. In aquaculture, light manipulation is com...
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | Microbiome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40168-021-01160-4 |
| _version_ | 1818825013529149440 |
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| author | Amy R. Ellison David Wilcockson Jo Cable |
| author_facet | Amy R. Ellison David Wilcockson Jo Cable |
| author_sort | Amy R. Ellison |
| collection | DOAJ |
| description | Abstract Background Circadian rhythms of host immune activity and their microbiomes are likely pivotal to health and disease resistance. The integration of chronotherapeutic approaches to disease mitigation in managed animals, however, is yet to be realised. In aquaculture, light manipulation is commonly used to enhance growth and control reproduction but may have unknown negative consequences for animal health. Infectious diseases are a major barrier to sustainable aquaculture and understanding the circadian dynamics of fish immunity and crosstalk with the microbiome is urgently needed. Results Here, using rainbow trout (Oncorhynchus mykiss) as a model, we combine 16S rRNA metabarcoding, metagenomic sequencing and direct mRNA quantification methods to simultaneously characterise the circadian dynamics of skin clock and immune gene expression, and daily changes of skin microbiota. We demonstrate daily rhythms in fish skin immune expression and microbiomes, which are modulated by photoperiod and parasitic lice infection. We identify putative associations of host clock and immune gene profiles with microbial composition. Our results suggest circadian perturbation, that shifts the magnitude and timing of immune and microbiota activity, is detrimental to fish health. Conclusions The substantial circadian dynamics and fish host expression-microbiome relationships we find represent a valuable foundation for investigating the utility of chronotherapies in aquaculture, and more broadly contributes to our understanding of the role of microbiomes in circadian health of vertebrates. Video Abstract |
| first_indexed | 2024-12-19T00:05:01Z |
| format | Article |
| id | doaj.art-35add0e8cfd84c0488b44091bf62f0ee |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-12-19T00:05:01Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-35add0e8cfd84c0488b44091bf62f0ee2022-12-21T20:46:16ZengBMCMicrobiome2049-26182021-11-019111810.1186/s40168-021-01160-4Circadian dynamics of the teleost skin immune-microbiome interfaceAmy R. Ellison0David Wilcockson1Jo Cable2School of Natural Sciences, Bangor UniversityInstitute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth UniversitySchool of Biosciences, Cardiff UniversityAbstract Background Circadian rhythms of host immune activity and their microbiomes are likely pivotal to health and disease resistance. The integration of chronotherapeutic approaches to disease mitigation in managed animals, however, is yet to be realised. In aquaculture, light manipulation is commonly used to enhance growth and control reproduction but may have unknown negative consequences for animal health. Infectious diseases are a major barrier to sustainable aquaculture and understanding the circadian dynamics of fish immunity and crosstalk with the microbiome is urgently needed. Results Here, using rainbow trout (Oncorhynchus mykiss) as a model, we combine 16S rRNA metabarcoding, metagenomic sequencing and direct mRNA quantification methods to simultaneously characterise the circadian dynamics of skin clock and immune gene expression, and daily changes of skin microbiota. We demonstrate daily rhythms in fish skin immune expression and microbiomes, which are modulated by photoperiod and parasitic lice infection. We identify putative associations of host clock and immune gene profiles with microbial composition. Our results suggest circadian perturbation, that shifts the magnitude and timing of immune and microbiota activity, is detrimental to fish health. Conclusions The substantial circadian dynamics and fish host expression-microbiome relationships we find represent a valuable foundation for investigating the utility of chronotherapies in aquaculture, and more broadly contributes to our understanding of the role of microbiomes in circadian health of vertebrates. Video Abstracthttps://doi.org/10.1186/s40168-021-01160-4Circadian rhythmClock gene expressionMicrobiomeParasite infectionFishAquaculture |
| spellingShingle | Amy R. Ellison David Wilcockson Jo Cable Circadian dynamics of the teleost skin immune-microbiome interface Microbiome Circadian rhythm Clock gene expression Microbiome Parasite infection Fish Aquaculture |
| title | Circadian dynamics of the teleost skin immune-microbiome interface |
| title_full | Circadian dynamics of the teleost skin immune-microbiome interface |
| title_fullStr | Circadian dynamics of the teleost skin immune-microbiome interface |
| title_full_unstemmed | Circadian dynamics of the teleost skin immune-microbiome interface |
| title_short | Circadian dynamics of the teleost skin immune-microbiome interface |
| title_sort | circadian dynamics of the teleost skin immune microbiome interface |
| topic | Circadian rhythm Clock gene expression Microbiome Parasite infection Fish Aquaculture |
| url | https://doi.org/10.1186/s40168-021-01160-4 |
| work_keys_str_mv | AT amyrellison circadiandynamicsoftheteleostskinimmunemicrobiomeinterface AT davidwilcockson circadiandynamicsoftheteleostskinimmunemicrobiomeinterface AT jocable circadiandynamicsoftheteleostskinimmunemicrobiomeinterface |