Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound
Abstract Background Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. Th...
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-021-03373-4 |
| _version_ | 1818903522950774784 |
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| author | Md. Aminul Islam Shahed Zaman Kushal Biswas Md. Yusuf Al-Amin Md. Kamrul Hasan A. H. M. K. Alam Toshihisa Tanaka Golam Sadik |
| author_facet | Md. Aminul Islam Shahed Zaman Kushal Biswas Md. Yusuf Al-Amin Md. Kamrul Hasan A. H. M. K. Alam Toshihisa Tanaka Golam Sadik |
| author_sort | Md. Aminul Islam |
| collection | DOAJ |
| description | Abstract Background Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. The present study was undertaken to evaluate the cholinesterase inhibitory and antioxidant potential of W. chinensis. Methods The extract and fractions of the plant were evaluated for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method. The antioxidant activity was assessed in several in vitro models/assays such as reducing power, total antioxidant capacity, total phenolic and flavonoid content, scavenging of 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of brain lipid peroxidation. Chromatographic and spectroscopic methods were used to isolate and identify the active compound from the extract. Results Among the fractions, aqueous fraction (AQF) and ethylacetate fraction (EAF) exhibited high inhibition against acetylcholinesterase (IC50: 40.02 ± 0.16 μg/ml and 57.76 ± 0.37 μg/ml) and butyrylcholinesterase (IC50: 31.79 ± 0.18 μg/ml and 48.41 ± 0.05 μg/ml). Similarly, the EAF and AQF had high content of phenolics and flavonoids and possess strong antioxidant activity in several antioxidant assays including DPPH and hydroxyl radical scavenging, reducing power and total antioxidant activity. They effectively inhibited the peroxidation of brain lipid in vitro with IC50 values of 45.20 ± 0.10 μg/ml and 25.53 ± 0.04 μg/ml, respectively. A significant correlation was observed between total flavonoids and antioxidant and cholinesterase inhibitory activity. Activity guided chromatographic separation led to the isolation of a major active compound from the EAF and its structure was elucidated as apigenin by spectral analysis. Conclusions The potential ability of W. chinensis to inhibit the cholinesterase activity and peroxidation of lipids suggest that the plant might be useful for the management of AD. |
| first_indexed | 2024-12-19T20:52:53Z |
| format | Article |
| id | doaj.art-35b442339e1d40e19dd93fcce15b8450 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-19T20:52:53Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-35b442339e1d40e19dd93fcce15b84502022-12-21T20:06:03ZengBMCBMC Complementary Medicine and Therapies2662-76712021-07-0121111210.1186/s12906-021-03373-4Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compoundMd. Aminul Islam0Shahed Zaman1Kushal Biswas2Md. Yusuf Al-Amin3Md. Kamrul Hasan4A. H. M. K. Alam5Toshihisa Tanaka6Golam Sadik7Department of Chemistry, University of RajshahiDepartment of Chemistry, University of RajshahiDepartment of Pharmacy, University of RajshahiDepartment of Pharmacy, University of RajshahiDepartment of Pharmacy, University of RajshahiDepartment of Pharmacy, University of RajshahiPsychiatry, Graduate School of MedicineDepartment of Pharmacy, University of RajshahiAbstract Background Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. The present study was undertaken to evaluate the cholinesterase inhibitory and antioxidant potential of W. chinensis. Methods The extract and fractions of the plant were evaluated for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method. The antioxidant activity was assessed in several in vitro models/assays such as reducing power, total antioxidant capacity, total phenolic and flavonoid content, scavenging of 2,2′-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of brain lipid peroxidation. Chromatographic and spectroscopic methods were used to isolate and identify the active compound from the extract. Results Among the fractions, aqueous fraction (AQF) and ethylacetate fraction (EAF) exhibited high inhibition against acetylcholinesterase (IC50: 40.02 ± 0.16 μg/ml and 57.76 ± 0.37 μg/ml) and butyrylcholinesterase (IC50: 31.79 ± 0.18 μg/ml and 48.41 ± 0.05 μg/ml). Similarly, the EAF and AQF had high content of phenolics and flavonoids and possess strong antioxidant activity in several antioxidant assays including DPPH and hydroxyl radical scavenging, reducing power and total antioxidant activity. They effectively inhibited the peroxidation of brain lipid in vitro with IC50 values of 45.20 ± 0.10 μg/ml and 25.53 ± 0.04 μg/ml, respectively. A significant correlation was observed between total flavonoids and antioxidant and cholinesterase inhibitory activity. Activity guided chromatographic separation led to the isolation of a major active compound from the EAF and its structure was elucidated as apigenin by spectral analysis. Conclusions The potential ability of W. chinensis to inhibit the cholinesterase activity and peroxidation of lipids suggest that the plant might be useful for the management of AD.https://doi.org/10.1186/s12906-021-03373-4Wedelia chinensisAlzheimer’s diseaseCholinesterase inhibitionAntioxidant activityApigenin |
| spellingShingle | Md. Aminul Islam Shahed Zaman Kushal Biswas Md. Yusuf Al-Amin Md. Kamrul Hasan A. H. M. K. Alam Toshihisa Tanaka Golam Sadik Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound BMC Complementary Medicine and Therapies Wedelia chinensis Alzheimer’s disease Cholinesterase inhibition Antioxidant activity Apigenin |
| title | Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound |
| title_full | Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound |
| title_fullStr | Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound |
| title_full_unstemmed | Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound |
| title_short | Evaluation of cholinesterase inhibitory and antioxidant activity of Wedelia chinensis and isolation of apigenin as an active compound |
| title_sort | evaluation of cholinesterase inhibitory and antioxidant activity of wedelia chinensis and isolation of apigenin as an active compound |
| topic | Wedelia chinensis Alzheimer’s disease Cholinesterase inhibition Antioxidant activity Apigenin |
| url | https://doi.org/10.1186/s12906-021-03373-4 |
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