Road Salt versus Urban Snow Effects on Lake Microbial Communities
Freshwater salinization is an ongoing concern for north temperate lakes; however, little is known about its impacts on microbial communities, particularly for bacteria. We tested the hypotheses that road de-icing salt induces changes in the microbial community structure of lake plankton, and that ch...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/4/803 |
| _version_ | 1797444698122485760 |
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| author | Isabelle B. Fournier Connie Lovejoy Warwick F. Vincent |
| author_facet | Isabelle B. Fournier Connie Lovejoy Warwick F. Vincent |
| author_sort | Isabelle B. Fournier |
| collection | DOAJ |
| description | Freshwater salinization is an ongoing concern for north temperate lakes; however, little is known about its impacts on microbial communities, particularly for bacteria. We tested the hypotheses that road de-icing salt induces changes in the microbial community structure of lake plankton, and that changes due to chloride would differ from those due to urban snowmelt because of additional chemicals in the snowmelt. In a laboratory incubator experiment, an overwintering plankton community in lake water was exposed for two weeks to either NaCl or municipal road snow with the same level of chloride. Microbial community structure as determined by 16S (prokaryotes) and 18S (eukaryotes) rRNA transcript analysis showed changes in response to the chloride-only enrichment, with some rare taxa becoming more prominent. Consistent with our hypothesis, the salt and the snow treatments induced different community changes. These results indicate that ecotoxicology assays based on a single salt addition may not reflect the in situ effects of salt-contaminated urban snow, and that the combined chemical effects of urban snowmelt require direct testing. |
| first_indexed | 2024-03-09T13:16:14Z |
| format | Article |
| id | doaj.art-35b522bc4ce7472ca07d77d1da64c6ef |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:14Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-35b522bc4ce7472ca07d77d1da64c6ef2023-11-30T21:35:41ZengMDPI AGMicroorganisms2076-26072022-04-0110480310.3390/microorganisms10040803Road Salt versus Urban Snow Effects on Lake Microbial CommunitiesIsabelle B. Fournier0Connie Lovejoy1Warwick F. Vincent2Departement de Biologie, Institut de Biologie Integrative et des Systemes (IBIS), Centre D’etudes Nordiques (CEN), Universite Laval, Quebec City, QC G1V 0A6, CanadaDepartement de Biologie, Institut de Biologie Integrative et des Systemes (IBIS), Centre D’etudes Nordiques (CEN), Universite Laval, Quebec City, QC G1V 0A6, CanadaDepartement de Biologie, Institut de Biologie Integrative et des Systemes (IBIS), Centre D’etudes Nordiques (CEN), Universite Laval, Quebec City, QC G1V 0A6, CanadaFreshwater salinization is an ongoing concern for north temperate lakes; however, little is known about its impacts on microbial communities, particularly for bacteria. We tested the hypotheses that road de-icing salt induces changes in the microbial community structure of lake plankton, and that changes due to chloride would differ from those due to urban snowmelt because of additional chemicals in the snowmelt. In a laboratory incubator experiment, an overwintering plankton community in lake water was exposed for two weeks to either NaCl or municipal road snow with the same level of chloride. Microbial community structure as determined by 16S (prokaryotes) and 18S (eukaryotes) rRNA transcript analysis showed changes in response to the chloride-only enrichment, with some rare taxa becoming more prominent. Consistent with our hypothesis, the salt and the snow treatments induced different community changes. These results indicate that ecotoxicology assays based on a single salt addition may not reflect the in situ effects of salt-contaminated urban snow, and that the combined chemical effects of urban snowmelt require direct testing.https://www.mdpi.com/2076-2607/10/4/803bacteriachloridemicrobial eukaryotesplanktonurban lakesroad salts |
| spellingShingle | Isabelle B. Fournier Connie Lovejoy Warwick F. Vincent Road Salt versus Urban Snow Effects on Lake Microbial Communities Microorganisms bacteria chloride microbial eukaryotes plankton urban lakes road salts |
| title | Road Salt versus Urban Snow Effects on Lake Microbial Communities |
| title_full | Road Salt versus Urban Snow Effects on Lake Microbial Communities |
| title_fullStr | Road Salt versus Urban Snow Effects on Lake Microbial Communities |
| title_full_unstemmed | Road Salt versus Urban Snow Effects on Lake Microbial Communities |
| title_short | Road Salt versus Urban Snow Effects on Lake Microbial Communities |
| title_sort | road salt versus urban snow effects on lake microbial communities |
| topic | bacteria chloride microbial eukaryotes plankton urban lakes road salts |
| url | https://www.mdpi.com/2076-2607/10/4/803 |
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