Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis
Point-of-care testing (POCT) devices are becoming increasingly popular in the medical community as an alternative to conventional laboratory testing, especially for home treatments or other forms of outpatient care. Multiple-use chemical sensors with minimal requirements for disposables are among th...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/10/6/232 |
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| author | Artur Kuznetsov Aleksandr Frorip Alar Sünter Vadim Korsakov Georgii Konoplev Oksana Stepanova Natalia Roschina Nikolay Ovsyannikov Daniil Lialin Roman Gerasimchuk Alexander Dmitriev Nikolay Mukhin Soeren Hirsch |
| author_facet | Artur Kuznetsov Aleksandr Frorip Alar Sünter Vadim Korsakov Georgii Konoplev Oksana Stepanova Natalia Roschina Nikolay Ovsyannikov Daniil Lialin Roman Gerasimchuk Alexander Dmitriev Nikolay Mukhin Soeren Hirsch |
| author_sort | Artur Kuznetsov |
| collection | DOAJ |
| description | Point-of-care testing (POCT) devices are becoming increasingly popular in the medical community as an alternative to conventional laboratory testing, especially for home treatments or other forms of outpatient care. Multiple-use chemical sensors with minimal requirements for disposables are among the most practical and cost-effective POC diagnostic instruments, especially in managing chronic conditions. An affordable, simple, and easy-to-use optical sensor based on fast protein liquid chromatography with direct UV absorption detection was developed for the rapid determination of the total protein concentration in effluent peritoneal dialysate and for the assessment of protein losses in end-stage renal disease (ESRD) patients on constant ambulatory peritoneal dialysis (CAPD). The sensor employs non-disposable PD-10 desalting columns for the separation of molecules with different molecular weights and a deep UV LED (maximum at 285 nm) as a light source for optical detection. The analytic procedure is relatively simple, takes 10–15 min, and potentially can be performed by patients themselves or nursing staff without laboratory training. Preliminary clinical trials on a group of 23 patients on CAPD revealed a good concordance between the protein concentrations in dialysate samples measured with the sensor and an automated biochemical analyzer; the mean relative error was about 10%, which is comparable with routine clinical laboratory methods. |
| first_indexed | 2024-03-10T00:07:56Z |
| format | Article |
| id | doaj.art-35b68869d7b24a358a84ba58862cbb31 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T00:07:56Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-35b68869d7b24a358a84ba58862cbb312023-11-23T16:04:22ZengMDPI AGChemosensors2227-90402022-06-0110623210.3390/chemosensors10060232Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal DialysisArtur Kuznetsov0Aleksandr Frorip1Alar Sünter2Vadim Korsakov3Georgii Konoplev4Oksana Stepanova5Natalia Roschina6Nikolay Ovsyannikov7Daniil Lialin8Roman Gerasimchuk9Alexander Dmitriev10Nikolay Mukhin11Soeren Hirsch12AS Ldiamon, 50411 Tartu, EstoniaAS Ldiamon, 50411 Tartu, EstoniaAS Ldiamon, 50411 Tartu, EstoniaJeko Disain OÜ, 51014 Tartu, EstoniaDepartment of Photonics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, RussiaDepartment of Photonics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, RussiaDepartment of Photonics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, RussiaDepartment of Photonics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, RussiaDepartment of Photonics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, RussiaSaint Petersburg City Mariinsky Hospital, 199004 Saint Petersburg, RussiaFederal State Budgetary Scientific Institution “Institute of Experimental Medicine” (FSBSI “IEM”), 197376 Saint Petersburg, RussiaDepartment of Engineering, University of Applied Sciences Brandenburg, 14770 Brandenburg an der Havel, GermanyDepartment of Engineering, University of Applied Sciences Brandenburg, 14770 Brandenburg an der Havel, GermanyPoint-of-care testing (POCT) devices are becoming increasingly popular in the medical community as an alternative to conventional laboratory testing, especially for home treatments or other forms of outpatient care. Multiple-use chemical sensors with minimal requirements for disposables are among the most practical and cost-effective POC diagnostic instruments, especially in managing chronic conditions. An affordable, simple, and easy-to-use optical sensor based on fast protein liquid chromatography with direct UV absorption detection was developed for the rapid determination of the total protein concentration in effluent peritoneal dialysate and for the assessment of protein losses in end-stage renal disease (ESRD) patients on constant ambulatory peritoneal dialysis (CAPD). The sensor employs non-disposable PD-10 desalting columns for the separation of molecules with different molecular weights and a deep UV LED (maximum at 285 nm) as a light source for optical detection. The analytic procedure is relatively simple, takes 10–15 min, and potentially can be performed by patients themselves or nursing staff without laboratory training. Preliminary clinical trials on a group of 23 patients on CAPD revealed a good concordance between the protein concentrations in dialysate samples measured with the sensor and an automated biochemical analyzer; the mean relative error was about 10%, which is comparable with routine clinical laboratory methods.https://www.mdpi.com/2227-9040/10/6/232optical chemical sensorfast-protein liquid chromatographyUV LEDend-stage renal diseaseperitoneal dialysisperitoneal protein loss |
| spellingShingle | Artur Kuznetsov Aleksandr Frorip Alar Sünter Vadim Korsakov Georgii Konoplev Oksana Stepanova Natalia Roschina Nikolay Ovsyannikov Daniil Lialin Roman Gerasimchuk Alexander Dmitriev Nikolay Mukhin Soeren Hirsch Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis Chemosensors optical chemical sensor fast-protein liquid chromatography UV LED end-stage renal disease peritoneal dialysis peritoneal protein loss |
| title | Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis |
| title_full | Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis |
| title_fullStr | Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis |
| title_full_unstemmed | Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis |
| title_short | Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis |
| title_sort | optical chemical sensor based on fast protein liquid chromatography for regular peritoneal protein loss assessment in end stage renal disease patients on continuous ambulatory peritoneal dialysis |
| topic | optical chemical sensor fast-protein liquid chromatography UV LED end-stage renal disease peritoneal dialysis peritoneal protein loss |
| url | https://www.mdpi.com/2227-9040/10/6/232 |
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