Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for <i>Ananas comosus</i> L. Merr. Hybrid “MD2”
The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (<i>Ananas comosus</i> L.) plants, with a high rate of multiplication and that would preserve the genetic identity of the donor genotype (Hybrid ‘MD2’) in the regenerated plants...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/5/713 |
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| author | Argelys Kessel-Domini Daisy Pérez-Brito Adolfo Guzmán-Antonio Felipe A. Barredo-Pool Javier O. Mijangos-Cortés Lourdes Georgina Iglesias-Andreu Alberto Cortés-Velázquez Adriana Canto-Flick Susana A. Avilés-Viñas Yaritza Rodríguez-Llanes Nancy Santana-Buzzy |
| author_facet | Argelys Kessel-Domini Daisy Pérez-Brito Adolfo Guzmán-Antonio Felipe A. Barredo-Pool Javier O. Mijangos-Cortés Lourdes Georgina Iglesias-Andreu Alberto Cortés-Velázquez Adriana Canto-Flick Susana A. Avilés-Viñas Yaritza Rodríguez-Llanes Nancy Santana-Buzzy |
| author_sort | Argelys Kessel-Domini |
| collection | DOAJ |
| description | The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (<i>Ananas comosus</i> L.) plants, with a high rate of multiplication and that would preserve the genetic identity of the donor genotype (Hybrid ‘MD2’) in the regenerated plants. Ten treatments, with different concentrations of 2,4-Dichlorophenoxyacetic (2,4-D) and Picloram (P), in the absence or presence of 6-Benzylaminopurine (BAP), were used for in vitro morphogenesis induction, as well as histological and molecular techniques, in order to characterize the morphogenic responses induced. Significant differences between treatments tested, to induce callus and buds, were assessed by the Kruskal Wallis method and the Mann–Whitney U-tests. Different pineapple regeneration routes were identified, showing the high regeneration potential of this species. The medium containing 2 mg L<sup>−1</sup> 2,4-D and 2 mg L<sup>−1</sup> BAP, where indirect somatic embryogenesis occurred, was selected as the most efficient treatment, with an average of 120 somatic embryos per explant, differing significantly from the rest of the treatments. It was also demonstrated that the pineapple plants regenerated in vitro preserved the genetic identity of the donor genotype, which represents a high degree of confidence for the application of indirect somatic embryogenesis for <i>A. comusus</i> clonal propagation. |
| first_indexed | 2024-03-10T03:31:07Z |
| format | Article |
| id | doaj.art-35b694cb604347029d0c19642a172af2 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T03:31:07Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-35b694cb604347029d0c19642a172af22023-11-23T09:40:36ZengMDPI AGAgriculture2077-04722022-05-0112571310.3390/agriculture12050713Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for <i>Ananas comosus</i> L. Merr. Hybrid “MD2”Argelys Kessel-Domini0Daisy Pérez-Brito1Adolfo Guzmán-Antonio2Felipe A. Barredo-Pool3Javier O. Mijangos-Cortés4Lourdes Georgina Iglesias-Andreu5Alberto Cortés-Velázquez6Adriana Canto-Flick7Susana A. Avilés-Viñas8Yaritza Rodríguez-Llanes9Nancy Santana-Buzzy10Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoGeMBio, Grupo de Estudios Moleculares Aplicados a la Biología, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Biotecnología, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Recursos Naturales, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoInstituto de Biotecnología y Ecología Aplicada, Campus para la Cultura, Universidad Veracruzana, las Artes y el Deporte. Av. de las Culturas Veracruzanas No. 101. Colonia Emiliano Zapata, Xalapa-Enríquez 91090, MexicoGeMBio, Grupo de Estudios Moleculares Aplicados a la Biología, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, MexicoThe objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (<i>Ananas comosus</i> L.) plants, with a high rate of multiplication and that would preserve the genetic identity of the donor genotype (Hybrid ‘MD2’) in the regenerated plants. Ten treatments, with different concentrations of 2,4-Dichlorophenoxyacetic (2,4-D) and Picloram (P), in the absence or presence of 6-Benzylaminopurine (BAP), were used for in vitro morphogenesis induction, as well as histological and molecular techniques, in order to characterize the morphogenic responses induced. Significant differences between treatments tested, to induce callus and buds, were assessed by the Kruskal Wallis method and the Mann–Whitney U-tests. Different pineapple regeneration routes were identified, showing the high regeneration potential of this species. The medium containing 2 mg L<sup>−1</sup> 2,4-D and 2 mg L<sup>−1</sup> BAP, where indirect somatic embryogenesis occurred, was selected as the most efficient treatment, with an average of 120 somatic embryos per explant, differing significantly from the rest of the treatments. It was also demonstrated that the pineapple plants regenerated in vitro preserved the genetic identity of the donor genotype, which represents a high degree of confidence for the application of indirect somatic embryogenesis for <i>A. comusus</i> clonal propagation.https://www.mdpi.com/2077-0472/12/5/713<i>Ananas comosus</i>somatic embryogenesismicropropagationorganogenesisSSR markersISSR markers |
| spellingShingle | Argelys Kessel-Domini Daisy Pérez-Brito Adolfo Guzmán-Antonio Felipe A. Barredo-Pool Javier O. Mijangos-Cortés Lourdes Georgina Iglesias-Andreu Alberto Cortés-Velázquez Adriana Canto-Flick Susana A. Avilés-Viñas Yaritza Rodríguez-Llanes Nancy Santana-Buzzy Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for <i>Ananas comosus</i> L. Merr. Hybrid “MD2” Agriculture <i>Ananas comosus</i> somatic embryogenesis micropropagation organogenesis SSR markers ISSR markers |
| title | Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for <i>Ananas comosus</i> L. Merr. Hybrid “MD2” |
| title_full | Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for <i>Ananas comosus</i> L. Merr. Hybrid “MD2” |
| title_fullStr | Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for <i>Ananas comosus</i> L. Merr. Hybrid “MD2” |
| title_full_unstemmed | Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for <i>Ananas comosus</i> L. Merr. Hybrid “MD2” |
| title_short | Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for <i>Ananas comosus</i> L. Merr. Hybrid “MD2” |
| title_sort | indirect somatic embryogenesis an efficient and genetically reliable clonal propagation system for i ananas comosus i l merr hybrid md2 |
| topic | <i>Ananas comosus</i> somatic embryogenesis micropropagation organogenesis SSR markers ISSR markers |
| url | https://www.mdpi.com/2077-0472/12/5/713 |
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