Fast-Track Introgression of “QTL-hotspot” for Root Traits and Other Drought Tolerance Traits in JG 11, an Elite and Leading Variety of Chickpea
A “” containing quantitative trait loci (QTL) for several root and drought tolerance traits was transferred through marker-assisted backcrossing into JG 11, a leading variety of chickpea ( L.) in India from the donor parent ICC 4958. Foreground selection with up to three simple sequence repeat marke...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-11-01
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| Series: | The Plant Genome |
| Online Access: | https://dl.sciencesocieties.org/publications/tpg/articles/6/3/plantgenome2013.07.0022 |
| _version_ | 1818205140015906816 |
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| author | Rajeev K. Varshney Pooran M. Gaur Siva K. Chamarthi L. Krishnamurthy Shailesh Tripathi Junichi Kashiwagi Srinivasan Samineni Vikas K. Singh Mahendar Thudi Deepa Jaganathan |
| author_facet | Rajeev K. Varshney Pooran M. Gaur Siva K. Chamarthi L. Krishnamurthy Shailesh Tripathi Junichi Kashiwagi Srinivasan Samineni Vikas K. Singh Mahendar Thudi Deepa Jaganathan |
| author_sort | Rajeev K. Varshney |
| collection | DOAJ |
| description | A “” containing quantitative trait loci (QTL) for several root and drought tolerance traits was transferred through marker-assisted backcrossing into JG 11, a leading variety of chickpea ( L.) in India from the donor parent ICC 4958. Foreground selection with up to three simple sequence repeat markers, namely TAA170, ICCM0249, and STMS11, and background selection with up to 10 amplified fragment length polymorphism primer combinations was undertaken. After undertaking three backcrosses with foreground and background selection and selfing, 29 BCF plants homozygous for two markers (ICCM0249 and TAA170) were selected and referred as introgression lines (ILs). Root trait phenotyping of these ILs showed higher rooting depth (RDp) (average 115.21 ± 2.24 cm) in all 29 ILs, better root length density (RLD) (average 0.41 ± 0.02 cm cm) in 26 ILs, and higher root dry weight (RDW) (average 1.25 ± 0.08 g per cylinder) as compared to the recurrent parent, JG 11 (111.70 cm for RDp, 0.39 cm cm for RLD, and 1.10 g per cylinder for RDW), as well as the donor parent, ICC 4958 (114.20 cm for RDp, 0.45 cm cm for RLD, and 1.25 g per cylinder for RDW). These ILs, developed in 3 yr, after multilocation field trials may be released as improved variety with enhanced drought tolerance. |
| first_indexed | 2024-12-12T03:52:23Z |
| format | Article |
| id | doaj.art-35b6de74d966413aa27d085b9ec29109 |
| institution | Directory Open Access Journal |
| issn | 1940-3372 |
| language | English |
| last_indexed | 2024-12-12T03:52:23Z |
| publishDate | 2013-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj.art-35b6de74d966413aa27d085b9ec291092022-12-22T00:39:20ZengWileyThe Plant Genome1940-33722013-11-016310.3835/plantgenome2013.07.0022plantgenome2013.07.0022Fast-Track Introgression of “QTL-hotspot” for Root Traits and Other Drought Tolerance Traits in JG 11, an Elite and Leading Variety of ChickpeaRajeev K. VarshneyPooran M. GaurSiva K. ChamarthiL. KrishnamurthyShailesh TripathiJunichi KashiwagiSrinivasan SamineniVikas K. SinghMahendar ThudiDeepa JaganathanA “” containing quantitative trait loci (QTL) for several root and drought tolerance traits was transferred through marker-assisted backcrossing into JG 11, a leading variety of chickpea ( L.) in India from the donor parent ICC 4958. Foreground selection with up to three simple sequence repeat markers, namely TAA170, ICCM0249, and STMS11, and background selection with up to 10 amplified fragment length polymorphism primer combinations was undertaken. After undertaking three backcrosses with foreground and background selection and selfing, 29 BCF plants homozygous for two markers (ICCM0249 and TAA170) were selected and referred as introgression lines (ILs). Root trait phenotyping of these ILs showed higher rooting depth (RDp) (average 115.21 ± 2.24 cm) in all 29 ILs, better root length density (RLD) (average 0.41 ± 0.02 cm cm) in 26 ILs, and higher root dry weight (RDW) (average 1.25 ± 0.08 g per cylinder) as compared to the recurrent parent, JG 11 (111.70 cm for RDp, 0.39 cm cm for RLD, and 1.10 g per cylinder for RDW), as well as the donor parent, ICC 4958 (114.20 cm for RDp, 0.45 cm cm for RLD, and 1.25 g per cylinder for RDW). These ILs, developed in 3 yr, after multilocation field trials may be released as improved variety with enhanced drought tolerance.https://dl.sciencesocieties.org/publications/tpg/articles/6/3/plantgenome2013.07.0022 |
| spellingShingle | Rajeev K. Varshney Pooran M. Gaur Siva K. Chamarthi L. Krishnamurthy Shailesh Tripathi Junichi Kashiwagi Srinivasan Samineni Vikas K. Singh Mahendar Thudi Deepa Jaganathan Fast-Track Introgression of “QTL-hotspot” for Root Traits and Other Drought Tolerance Traits in JG 11, an Elite and Leading Variety of Chickpea The Plant Genome |
| title | Fast-Track Introgression of “QTL-hotspot” for Root Traits and Other Drought Tolerance Traits in JG 11, an Elite and Leading Variety of Chickpea |
| title_full | Fast-Track Introgression of “QTL-hotspot” for Root Traits and Other Drought Tolerance Traits in JG 11, an Elite and Leading Variety of Chickpea |
| title_fullStr | Fast-Track Introgression of “QTL-hotspot” for Root Traits and Other Drought Tolerance Traits in JG 11, an Elite and Leading Variety of Chickpea |
| title_full_unstemmed | Fast-Track Introgression of “QTL-hotspot” for Root Traits and Other Drought Tolerance Traits in JG 11, an Elite and Leading Variety of Chickpea |
| title_short | Fast-Track Introgression of “QTL-hotspot” for Root Traits and Other Drought Tolerance Traits in JG 11, an Elite and Leading Variety of Chickpea |
| title_sort | fast track introgression of qtl hotspot for root traits and other drought tolerance traits in jg 11 an elite and leading variety of chickpea |
| url | https://dl.sciencesocieties.org/publications/tpg/articles/6/3/plantgenome2013.07.0022 |
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