White Wine—Induced Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats

The vasodilatory activity and polyphenolic content of commercially available white wine is low compared to red wines. This study assessed the vasodilator potential of white wines produced by four different fermentation processes: (1) white wine produced by the standard procedure; (2) grapes left to...

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Main Authors: Zrinka Mihaljević, Toni Kujundžić, Vladimir Jukić, Ana Stupin, Mato Drenjančević, Ines Drenjančević
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Antioxidants
Subjects:
Online Access:https://www.mdpi.com/2076-3921/11/5/944
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author Zrinka Mihaljević
Toni Kujundžić
Vladimir Jukić
Ana Stupin
Mato Drenjančević
Ines Drenjančević
author_facet Zrinka Mihaljević
Toni Kujundžić
Vladimir Jukić
Ana Stupin
Mato Drenjančević
Ines Drenjančević
author_sort Zrinka Mihaljević
collection DOAJ
description The vasodilatory activity and polyphenolic content of commercially available white wine is low compared to red wines. This study assessed the vasodilator potential of white wines produced by four different fermentation processes: (1) white wine produced by the standard procedure; (2) grapes left to macerate completely for 30 days; (3) grapes left to macerate up to half of unfermented sugar; and (4) wine produced by cooling the must. All tested wine samples were analyzed for their phenolic content, antioxidant capacity, and ethanol content. Vasodilation was examined in the norepinephrine pre-contracted isolated rat aortas of male Sprague-Dawley rats randomly exposed to cumulative concentrations (0.1‰ to 8‰ final dilutions in organ baths) of each of the tested wine samples with or without quercetin and/or gallic acid supplementation, in the absence/presence of NOS inhibitor L-NAME. Standard procedure and the procedure involving must cooling gives wine with lower phenolic content, antioxidant capacity, and lower vasodilator potential, respectively. L-NAME inhibited vasodilation to all wine samples. Quercetin with or without gallic acid supplementation restored vasodilation. Results show that vasodilation to white wine is NO-dependent and suggest the possibility of increasing the antioxidant capacity and vasodilatory potential of white wine using different production procedures, depending on quercetin content.
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spelling doaj.art-35b972574d304086a14287197764a71b2023-11-23T09:52:09ZengMDPI AGAntioxidants2076-39212022-05-0111594410.3390/antiox11050944White Wine—Induced Endothelium-Dependent Vasorelaxation in Sprague-Dawley RatsZrinka Mihaljević0Toni Kujundžić1Vladimir Jukić2Ana Stupin3Mato Drenjančević4Ines Drenjančević5Institute and Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, CroatiaDepartment of Fruit Growing, Viticulture and Enology, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, CroatiaDepartment of Fruit Growing, Viticulture and Enology, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, CroatiaInstitute and Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, CroatiaDepartment of Fruit Growing, Viticulture and Enology, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, CroatiaInstitute and Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, 31000 Osijek, CroatiaThe vasodilatory activity and polyphenolic content of commercially available white wine is low compared to red wines. This study assessed the vasodilator potential of white wines produced by four different fermentation processes: (1) white wine produced by the standard procedure; (2) grapes left to macerate completely for 30 days; (3) grapes left to macerate up to half of unfermented sugar; and (4) wine produced by cooling the must. All tested wine samples were analyzed for their phenolic content, antioxidant capacity, and ethanol content. Vasodilation was examined in the norepinephrine pre-contracted isolated rat aortas of male Sprague-Dawley rats randomly exposed to cumulative concentrations (0.1‰ to 8‰ final dilutions in organ baths) of each of the tested wine samples with or without quercetin and/or gallic acid supplementation, in the absence/presence of NOS inhibitor L-NAME. Standard procedure and the procedure involving must cooling gives wine with lower phenolic content, antioxidant capacity, and lower vasodilator potential, respectively. L-NAME inhibited vasodilation to all wine samples. Quercetin with or without gallic acid supplementation restored vasodilation. Results show that vasodilation to white wine is NO-dependent and suggest the possibility of increasing the antioxidant capacity and vasodilatory potential of white wine using different production procedures, depending on quercetin content.https://www.mdpi.com/2076-3921/11/5/944vasodilationwineSprague-Dawley ratantioxidantsfermentation
spellingShingle Zrinka Mihaljević
Toni Kujundžić
Vladimir Jukić
Ana Stupin
Mato Drenjančević
Ines Drenjančević
White Wine—Induced Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats
Antioxidants
vasodilation
wine
Sprague-Dawley rat
antioxidants
fermentation
title White Wine—Induced Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats
title_full White Wine—Induced Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats
title_fullStr White Wine—Induced Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats
title_full_unstemmed White Wine—Induced Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats
title_short White Wine—Induced Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats
title_sort white wine induced endothelium dependent vasorelaxation in sprague dawley rats
topic vasodilation
wine
Sprague-Dawley rat
antioxidants
fermentation
url https://www.mdpi.com/2076-3921/11/5/944
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AT anastupin whitewineinducedendotheliumdependentvasorelaxationinspraguedawleyrats
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