Ex Vivo Expansion and Transplantation of Hematopoietic Stem/Progenitor Cells Supported by Mesenchymal Stem Cells from Human Umbilical Cord Blood
Human mesenchymal stem cells (MSCs) are multipotential and are detected in bone marrow (BM), adipose tissue, placenta, and umbilical cord blood (UCB). In this study, we examined the ability of UCB-derived MSCs (UCB-MSCs) to support ex vivo expansion of hematopoietic stem/progenitor cells (HSPCs) fro...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2007-07-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000007783465073 |
| _version_ | 1818535247770288128 |
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| author | Guo-Ping Huang Zhi-Jun Pan Bing-Bing Jia Qiang Zheng Chun-Gang Xie Jiang-Hong Gu Ian K. Mcniece Jin-Fu Wang |
| author_facet | Guo-Ping Huang Zhi-Jun Pan Bing-Bing Jia Qiang Zheng Chun-Gang Xie Jiang-Hong Gu Ian K. Mcniece Jin-Fu Wang |
| author_sort | Guo-Ping Huang |
| collection | DOAJ |
| description | Human mesenchymal stem cells (MSCs) are multipotential and are detected in bone marrow (BM), adipose tissue, placenta, and umbilical cord blood (UCB). In this study, we examined the ability of UCB-derived MSCs (UCB-MSCs) to support ex vivo expansion of hematopoietic stem/progenitor cells (HSPCs) from UCB and the engraftment of expanded HSPCs in NOD/SCID mice. The result showed that UCB-MSCs supported the proliferation and differentiation of CD34+ cells in vitro. The number of expanded total nucleated cells (TNCs) in MSC-based culture was twofold higher than cultures without MSC (control cultures). UCB-MSCs increased the expansion capabilities of CD34+ cells, long-term culture-initiating cells (LTC-ICs), granulocyte-macrophage colony-forming cells (GM-CFCs), and high proliferative potential colony-forming cells (HPP-CFCs) compared to control cultures. The expanded HSPCs were transplanted into lethally irradiated NOD/SCID mice to assess the effects of expanded cells on hematopoietic recovery. The number of white blood cells (WBCs) in the peripheral blood of mice transplanted with expanded cells from both the MSC-based and control cultures returned to pretreatment levels at day 25 posttransplant and then decreased. The WBC levels returned to pretreatment levels again at days 45–55 posttransplant. The level of human CD45+ cell engraftment in primary recipients transplanted with expanded cells from the MSC-based cultures was significantly higher than recipients transplanted with cells from the control cultures. Serial transplantation demonstrated that the expanded cells could establish long-term engraftment of hematopoietic cells. UCB-MSCs similar to those derived from adult bone marrow may provide novel targets for cellular and gene therapy. |
| first_indexed | 2024-12-11T18:22:18Z |
| format | Article |
| id | doaj.art-35ba6723662142ba9ca44a32070ed22f |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-11T18:22:18Z |
| publishDate | 2007-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-35ba6723662142ba9ca44a32070ed22f2022-12-22T00:55:12ZengSAGE PublishingCell Transplantation0963-68971555-38922007-07-011610.3727/000000007783465073Ex Vivo Expansion and Transplantation of Hematopoietic Stem/Progenitor Cells Supported by Mesenchymal Stem Cells from Human Umbilical Cord BloodGuo-Ping Huang0Zhi-Jun Pan1Bing-Bing Jia2Qiang Zheng3Chun-Gang Xie4Jiang-Hong Gu5Ian K. Mcniece6Jin-Fu Wang7 College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou 310058, P. R. China The Second Affiliated Hospital, Zhejiang University, Hangzhou 310006, P. R. China College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou 310058, P. R. China The Second Affiliated Hospital, Zhejiang University, Hangzhou 310006, P. R. China College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou 310058, P. R. China The Obstetrics Department, Hangzhou Traditional Chinese Medical Hospital, Hangzhou 310027, P. R. China Johns Hopkins Oncology Center, Division of Hematologic Malignancies, Baltimore, MD 21231, USA College of Life Sciences, Zi Jin Gang Campus, Zhejiang University, Hangzhou 310058, P. R. ChinaHuman mesenchymal stem cells (MSCs) are multipotential and are detected in bone marrow (BM), adipose tissue, placenta, and umbilical cord blood (UCB). In this study, we examined the ability of UCB-derived MSCs (UCB-MSCs) to support ex vivo expansion of hematopoietic stem/progenitor cells (HSPCs) from UCB and the engraftment of expanded HSPCs in NOD/SCID mice. The result showed that UCB-MSCs supported the proliferation and differentiation of CD34+ cells in vitro. The number of expanded total nucleated cells (TNCs) in MSC-based culture was twofold higher than cultures without MSC (control cultures). UCB-MSCs increased the expansion capabilities of CD34+ cells, long-term culture-initiating cells (LTC-ICs), granulocyte-macrophage colony-forming cells (GM-CFCs), and high proliferative potential colony-forming cells (HPP-CFCs) compared to control cultures. The expanded HSPCs were transplanted into lethally irradiated NOD/SCID mice to assess the effects of expanded cells on hematopoietic recovery. The number of white blood cells (WBCs) in the peripheral blood of mice transplanted with expanded cells from both the MSC-based and control cultures returned to pretreatment levels at day 25 posttransplant and then decreased. The WBC levels returned to pretreatment levels again at days 45–55 posttransplant. The level of human CD45+ cell engraftment in primary recipients transplanted with expanded cells from the MSC-based cultures was significantly higher than recipients transplanted with cells from the control cultures. Serial transplantation demonstrated that the expanded cells could establish long-term engraftment of hematopoietic cells. UCB-MSCs similar to those derived from adult bone marrow may provide novel targets for cellular and gene therapy.https://doi.org/10.3727/000000007783465073 |
| spellingShingle | Guo-Ping Huang Zhi-Jun Pan Bing-Bing Jia Qiang Zheng Chun-Gang Xie Jiang-Hong Gu Ian K. Mcniece Jin-Fu Wang Ex Vivo Expansion and Transplantation of Hematopoietic Stem/Progenitor Cells Supported by Mesenchymal Stem Cells from Human Umbilical Cord Blood Cell Transplantation |
| title | Ex Vivo Expansion and Transplantation of Hematopoietic Stem/Progenitor Cells Supported by Mesenchymal Stem Cells from Human Umbilical Cord Blood |
| title_full | Ex Vivo Expansion and Transplantation of Hematopoietic Stem/Progenitor Cells Supported by Mesenchymal Stem Cells from Human Umbilical Cord Blood |
| title_fullStr | Ex Vivo Expansion and Transplantation of Hematopoietic Stem/Progenitor Cells Supported by Mesenchymal Stem Cells from Human Umbilical Cord Blood |
| title_full_unstemmed | Ex Vivo Expansion and Transplantation of Hematopoietic Stem/Progenitor Cells Supported by Mesenchymal Stem Cells from Human Umbilical Cord Blood |
| title_short | Ex Vivo Expansion and Transplantation of Hematopoietic Stem/Progenitor Cells Supported by Mesenchymal Stem Cells from Human Umbilical Cord Blood |
| title_sort | ex vivo expansion and transplantation of hematopoietic stem progenitor cells supported by mesenchymal stem cells from human umbilical cord blood |
| url | https://doi.org/10.3727/000000007783465073 |
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