Functional analysis of the dehydratase domains of the PUFA synthase from Emiliania huxleyi in Escherichia coli and Arabidopsis thaliana

Functional analysis of the dehydratase domains of the PUFA synthase from Emiliania huxleyi in Escherichia coli and Arabidopsis thaliana

Abstract Background Polyunsaturated fatty acid (PUFA) synthase is a multi-domain mega-enzyme that effectively synthesizes a series of PUFAs in marine microorganisms. The dehydratase (DH) domain of a PUFA synthase plays a crucial role in double bond positioning in fatty acids. Sequencing results of t...

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Bibliographic Details
Main Authors: Bihan Chen, Feng Wang, Xi Xie, Huifan Liu, Dongjie Liu, Lukai Ma, Gengsheng Xiao, Qin Wang
Format: Article
Language:English
Published: BMC 2022-11-01
Series:Biotechnology for Biofuels and Bioproducts
Subjects:
Polyunsaturated fatty acid synthase
Dehydratase domain
Emiliania huxleyi
Escherichia coli
Arabidopsis thaliana
Recombinant
Online Access:https://doi.org/10.1186/s13068-022-02223-w

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