Screening and optimization of Manganese peroxidase (MnP) production by Pseudoduganella violacea (SMB4), a bacterial isolate from Similipal Biosphere Reserve, Odisha and evaluation of Maillard reaction products degradation
The Maillard reaction products (Melanoidin) are major environmental pollutants of the distillery and fermentation industries. Manganese peroxidase (MnP) was found to be an efficient ligninolytic enzyme capable of degrading Maillard products. MnP can convert Mn2 ions in wood and soils to the more rea...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Sustainable Chemistry for the Environment |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949839223000093 |
| _version_ | 1797235385050333184 |
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| author | Nitish Kumar Kheti Subhashree Rath Hrudayanath Thatoi |
| author_facet | Nitish Kumar Kheti Subhashree Rath Hrudayanath Thatoi |
| author_sort | Nitish Kumar Kheti |
| collection | DOAJ |
| description | The Maillard reaction products (Melanoidin) are major environmental pollutants of the distillery and fermentation industries. Manganese peroxidase (MnP) was found to be an efficient ligninolytic enzyme capable of degrading Maillard products. MnP can convert Mn2 ions in wood and soils to the more reactive Mn2+ ions. The current study aims to isolate MnP producing bacteria from Similipal Biosphere Reserve (SBR), Odisha, and determine its biodegradation ability towards millards products. In total 10 ligninolytic bacterial colonies were isolated from the soil sample of SBR and their MnP activity was screened using phenol red dye as an indicator. The isolate, SMB4 was found to be the most efficient MnP-producing bacterium showing the highest MnP activity of 3.89 U/ml/min in un-optimized conditions. The bacterium was identified as Pseudoduganella violacea based on morphological, biochemical and molecular characterization. MnP activity of SMB4 was optimized using response surface methodology. Under optimized conditions SMB4 showed MnP activity of 6.12 U/ml/min which is 1.57 times from un-optimized condition. The isolate SMB4 was found to show significant decolorization of maillards products up to 83.68 % at temperature 37 °C within 192 h of incubation. The degraded product was analyzed using UV–vis and FTIR analyses. The current investigation suggests that MnP produced by Pseudoduganella violacea may be successfully exploited for bioremediation purposes to degrade Maillard products. |
| first_indexed | 2024-04-24T16:47:07Z |
| format | Article |
| id | doaj.art-35c5d39ce6734a498ee3f75523b464f6 |
| institution | Directory Open Access Journal |
| issn | 2949-8392 |
| language | English |
| last_indexed | 2024-04-24T16:47:07Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sustainable Chemistry for the Environment |
| spelling | doaj.art-35c5d39ce6734a498ee3f75523b464f62024-03-29T05:52:24ZengElsevierSustainable Chemistry for the Environment2949-83922023-08-012100009Screening and optimization of Manganese peroxidase (MnP) production by Pseudoduganella violacea (SMB4), a bacterial isolate from Similipal Biosphere Reserve, Odisha and evaluation of Maillard reaction products degradationNitish Kumar Kheti0Subhashree Rath1Hrudayanath Thatoi2Department of Biotechnology, Maharaja Sriram Chandra Bhanja Deo University, Takatpur, Baripada 757003, Odisha, IndiaDepartment of Biotechnology, Maharaja Sriram Chandra Bhanja Deo University, Takatpur, Baripada 757003, Odisha, IndiaCorresponding author.; Department of Biotechnology, Maharaja Sriram Chandra Bhanja Deo University, Takatpur, Baripada 757003, Odisha, IndiaThe Maillard reaction products (Melanoidin) are major environmental pollutants of the distillery and fermentation industries. Manganese peroxidase (MnP) was found to be an efficient ligninolytic enzyme capable of degrading Maillard products. MnP can convert Mn2 ions in wood and soils to the more reactive Mn2+ ions. The current study aims to isolate MnP producing bacteria from Similipal Biosphere Reserve (SBR), Odisha, and determine its biodegradation ability towards millards products. In total 10 ligninolytic bacterial colonies were isolated from the soil sample of SBR and their MnP activity was screened using phenol red dye as an indicator. The isolate, SMB4 was found to be the most efficient MnP-producing bacterium showing the highest MnP activity of 3.89 U/ml/min in un-optimized conditions. The bacterium was identified as Pseudoduganella violacea based on morphological, biochemical and molecular characterization. MnP activity of SMB4 was optimized using response surface methodology. Under optimized conditions SMB4 showed MnP activity of 6.12 U/ml/min which is 1.57 times from un-optimized condition. The isolate SMB4 was found to show significant decolorization of maillards products up to 83.68 % at temperature 37 °C within 192 h of incubation. The degraded product was analyzed using UV–vis and FTIR analyses. The current investigation suggests that MnP produced by Pseudoduganella violacea may be successfully exploited for bioremediation purposes to degrade Maillard products.http://www.sciencedirect.com/science/article/pii/S2949839223000093Manganese peroxidaseMaillard reaction productDecolorisationResponse surface methodologyFTIRUV-Vis |
| spellingShingle | Nitish Kumar Kheti Subhashree Rath Hrudayanath Thatoi Screening and optimization of Manganese peroxidase (MnP) production by Pseudoduganella violacea (SMB4), a bacterial isolate from Similipal Biosphere Reserve, Odisha and evaluation of Maillard reaction products degradation Sustainable Chemistry for the Environment Manganese peroxidase Maillard reaction product Decolorisation Response surface methodology FTIR UV-Vis |
| title | Screening and optimization of Manganese peroxidase (MnP) production by Pseudoduganella violacea (SMB4), a bacterial isolate from Similipal Biosphere Reserve, Odisha and evaluation of Maillard reaction products degradation |
| title_full | Screening and optimization of Manganese peroxidase (MnP) production by Pseudoduganella violacea (SMB4), a bacterial isolate from Similipal Biosphere Reserve, Odisha and evaluation of Maillard reaction products degradation |
| title_fullStr | Screening and optimization of Manganese peroxidase (MnP) production by Pseudoduganella violacea (SMB4), a bacterial isolate from Similipal Biosphere Reserve, Odisha and evaluation of Maillard reaction products degradation |
| title_full_unstemmed | Screening and optimization of Manganese peroxidase (MnP) production by Pseudoduganella violacea (SMB4), a bacterial isolate from Similipal Biosphere Reserve, Odisha and evaluation of Maillard reaction products degradation |
| title_short | Screening and optimization of Manganese peroxidase (MnP) production by Pseudoduganella violacea (SMB4), a bacterial isolate from Similipal Biosphere Reserve, Odisha and evaluation of Maillard reaction products degradation |
| title_sort | screening and optimization of manganese peroxidase mnp production by pseudoduganella violacea smb4 a bacterial isolate from similipal biosphere reserve odisha and evaluation of maillard reaction products degradation |
| topic | Manganese peroxidase Maillard reaction product Decolorisation Response surface methodology FTIR UV-Vis |
| url | http://www.sciencedirect.com/science/article/pii/S2949839223000093 |
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