Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences

The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the intera...

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Main Authors: Guya Diletta Marconi, Luigia Fonticoli, Ylenia Della Rocca, Stefano Oliva, Thangavelu Soundara Rajan, Oriana Trubiani, Giovanna Murmura, Francesca Diomede, Jacopo Pizzicannella
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:Biomedicines
Subjects:
Online Access:https://www.mdpi.com/2227-9059/9/11/1710
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author Guya Diletta Marconi
Luigia Fonticoli
Ylenia Della Rocca
Stefano Oliva
Thangavelu Soundara Rajan
Oriana Trubiani
Giovanna Murmura
Francesca Diomede
Jacopo Pizzicannella
author_facet Guya Diletta Marconi
Luigia Fonticoli
Ylenia Della Rocca
Stefano Oliva
Thangavelu Soundara Rajan
Oriana Trubiani
Giovanna Murmura
Francesca Diomede
Jacopo Pizzicannella
author_sort Guya Diletta Marconi
collection DOAJ
description The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the interaction of human periodontal ligament stem cells (hPDLSCs) and two different implant titanium surfaces topography (CTRL and TEST). This study was aimed at analyzing the cytotoxicity of the dental implant surfaces, the cellular adhesion capacity, and the improvement in the release of ECM molecules in an in vitro model. These parameters were carried out by means of the microscopic evaluation, viability assays, immunofluorescence, Western blot and RT-PCR investigations. The knowledge of the cell/implant interaction is essential for implant healing in order to obtain a more performing surfaces that promote the ECM release and provide the starting point to initiate the osseointegration process.
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spelling doaj.art-35c7a7a0787543e994a7a35543ada2952023-11-22T22:32:17ZengMDPI AGBiomedicines2227-90592021-11-01911171010.3390/biomedicines9111710Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular EvidencesGuya Diletta Marconi0Luigia Fonticoli1Ylenia Della Rocca2Stefano Oliva3Thangavelu Soundara Rajan4Oriana Trubiani5Giovanna Murmura6Francesca Diomede7Jacopo Pizzicannella8Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Biotechnology, Karpagam Academy of Higher Education, Coimbatore 641021, IndiaDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyCardiology Intensive Care Unit, “Ss. Annunziata” Hospital, ASL 02 Lanciano-Vasto-Chieti, 66100 Chieti, ItalyThe surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the interaction of human periodontal ligament stem cells (hPDLSCs) and two different implant titanium surfaces topography (CTRL and TEST). This study was aimed at analyzing the cytotoxicity of the dental implant surfaces, the cellular adhesion capacity, and the improvement in the release of ECM molecules in an in vitro model. These parameters were carried out by means of the microscopic evaluation, viability assays, immunofluorescence, Western blot and RT-PCR investigations. The knowledge of the cell/implant interaction is essential for implant healing in order to obtain a more performing surfaces that promote the ECM release and provide the starting point to initiate the osseointegration process.https://www.mdpi.com/2227-9059/9/11/1710osseointegrationosteogenesiscellular adhesiongene expressionextracellular matrix (ECM)
spellingShingle Guya Diletta Marconi
Luigia Fonticoli
Ylenia Della Rocca
Stefano Oliva
Thangavelu Soundara Rajan
Oriana Trubiani
Giovanna Murmura
Francesca Diomede
Jacopo Pizzicannella
Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
Biomedicines
osseointegration
osteogenesis
cellular adhesion
gene expression
extracellular matrix (ECM)
title Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_full Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_fullStr Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_full_unstemmed Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_short Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
title_sort enhanced extracellular matrix deposition on titanium implant surfaces cellular and molecular evidences
topic osseointegration
osteogenesis
cellular adhesion
gene expression
extracellular matrix (ECM)
url https://www.mdpi.com/2227-9059/9/11/1710
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