Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences
The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the intera...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/9/11/1710 |
| _version_ | 1827677220108238848 |
|---|---|
| author | Guya Diletta Marconi Luigia Fonticoli Ylenia Della Rocca Stefano Oliva Thangavelu Soundara Rajan Oriana Trubiani Giovanna Murmura Francesca Diomede Jacopo Pizzicannella |
| author_facet | Guya Diletta Marconi Luigia Fonticoli Ylenia Della Rocca Stefano Oliva Thangavelu Soundara Rajan Oriana Trubiani Giovanna Murmura Francesca Diomede Jacopo Pizzicannella |
| author_sort | Guya Diletta Marconi |
| collection | DOAJ |
| description | The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the interaction of human periodontal ligament stem cells (hPDLSCs) and two different implant titanium surfaces topography (CTRL and TEST). This study was aimed at analyzing the cytotoxicity of the dental implant surfaces, the cellular adhesion capacity, and the improvement in the release of ECM molecules in an in vitro model. These parameters were carried out by means of the microscopic evaluation, viability assays, immunofluorescence, Western blot and RT-PCR investigations. The knowledge of the cell/implant interaction is essential for implant healing in order to obtain a more performing surfaces that promote the ECM release and provide the starting point to initiate the osseointegration process. |
| first_indexed | 2024-03-10T05:41:01Z |
| format | Article |
| id | doaj.art-35c7a7a0787543e994a7a35543ada295 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T05:41:01Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-35c7a7a0787543e994a7a35543ada2952023-11-22T22:32:17ZengMDPI AGBiomedicines2227-90592021-11-01911171010.3390/biomedicines9111710Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular EvidencesGuya Diletta Marconi0Luigia Fonticoli1Ylenia Della Rocca2Stefano Oliva3Thangavelu Soundara Rajan4Oriana Trubiani5Giovanna Murmura6Francesca Diomede7Jacopo Pizzicannella8Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Biotechnology, Karpagam Academy of Higher Education, Coimbatore 641021, IndiaDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyDepartment of Innovative Technologies in Medicine and Dentistry, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, ItalyCardiology Intensive Care Unit, “Ss. Annunziata” Hospital, ASL 02 Lanciano-Vasto-Chieti, 66100 Chieti, ItalyThe surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the interaction of human periodontal ligament stem cells (hPDLSCs) and two different implant titanium surfaces topography (CTRL and TEST). This study was aimed at analyzing the cytotoxicity of the dental implant surfaces, the cellular adhesion capacity, and the improvement in the release of ECM molecules in an in vitro model. These parameters were carried out by means of the microscopic evaluation, viability assays, immunofluorescence, Western blot and RT-PCR investigations. The knowledge of the cell/implant interaction is essential for implant healing in order to obtain a more performing surfaces that promote the ECM release and provide the starting point to initiate the osseointegration process.https://www.mdpi.com/2227-9059/9/11/1710osseointegrationosteogenesiscellular adhesiongene expressionextracellular matrix (ECM) |
| spellingShingle | Guya Diletta Marconi Luigia Fonticoli Ylenia Della Rocca Stefano Oliva Thangavelu Soundara Rajan Oriana Trubiani Giovanna Murmura Francesca Diomede Jacopo Pizzicannella Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences Biomedicines osseointegration osteogenesis cellular adhesion gene expression extracellular matrix (ECM) |
| title | Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences |
| title_full | Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences |
| title_fullStr | Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences |
| title_full_unstemmed | Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences |
| title_short | Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences |
| title_sort | enhanced extracellular matrix deposition on titanium implant surfaces cellular and molecular evidences |
| topic | osseointegration osteogenesis cellular adhesion gene expression extracellular matrix (ECM) |
| url | https://www.mdpi.com/2227-9059/9/11/1710 |
| work_keys_str_mv | AT guyadilettamarconi enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT luigiafonticoli enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT yleniadellarocca enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT stefanooliva enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT thangavelusoundararajan enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT orianatrubiani enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT giovannamurmura enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT francescadiomede enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences AT jacopopizzicannella enhancedextracellularmatrixdepositionontitaniumimplantsurfacescellularandmolecularevidences |