Assays for the in vitro establishment of Swietenia macrophylla and Cedrela odorata
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| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Spanish |
| Published: |
Universidad Nacional de Colombia
2012-08-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/31695 |
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</style> <![endif]--> <p class="MsoNormal" style="text-align: justify; text-autospace: ideograph-numeric;"><strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213;" lang="ES">Título en español: Ensayos para el establecimiento <em style="mso-bidi-font-style: normal;">in vitro</em> de <em style="mso-bidi-font-style: normal;">Swietenia macrophylla</em> y <em style="mso-bidi-font-style: normal;">Cedrela odorata</em></span></strong><strong></strong></p> <p class="MsoNormal" style="text-align: justify; text-autospace: ideograph-numeric;"><strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US">Abstract: </span></strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US">Recalcitrance and contamination in Mahogany (<em>Swietenia macrophylla</em> King<span style="mso-bidi-font-style: italic;">)<em> </em></span>and Spanish cedar (<em>Cedrela odorata</em> L.) stem tissues are the main causes of its ineffective <em>in vitro</em> propagation. </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-ansi-language: EN-US;" lang="EN-US">The objectives of this research were: a) to evaluate </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US">sodium hypochlorite</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-ansi-language: EN-US;" lang="EN-US"> (<span style="mso-bidi-font-style: italic;">NaOCl) and </span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US">plant preservative mixture</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-ansi-language: EN-US; mso-bidi-font-style: italic;" lang="EN-US"> (PPM</span><sup><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: ";Lohit Hindi";; mso-ansi-language: EN-US;" lang="EN-US">®</span></sup><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-ansi-language: EN-US; mso-bidi-font-style: italic;" lang="EN-US">) as surface disinfectants and/or added to the culture medium for the <em>in vitro</em> </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-ansi-language: EN-US;" lang="EN-US">establishment of nodal explants taken from 10-year-old Mahogany<em> </em>and Spanish cedar<em> </em>plants, and b) to evaluate<span style="mso-bidi-font-style: italic;"> the <em>in vitro</em> response of such explants treated with </span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US">N6-benzylaminopurine (BAP) (0, 2.2, 4.4, 8.8, 17.7 </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213;" lang="ES">μ</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US">M), silver nitrate (AgNO<sub>3</sub>) (0, 3 mg l<sup>-1</sup>), activated charcoal (0, 1 g l<sup>-1</sup>) and vented caps. All the experiments were arranged in a completely randomized design. The NaOCl at 15%, for 20 min, as a surface sterilization or PPM</span><sup><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: ";Lohit Hindi";; mso-ansi-language: EN-US;" lang="EN-US">®</span></sup><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US"> at 2 ml l<sup>-1 </sup><span style="mso-spacerun: yes;"> </span>into the culture medium, were the best treatments to reduce contamination for both species. For Mahogany<em> </em>explants, BAP at 17.7 </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213;" lang="ES">μ</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US">M resulted in higher percentages of bud breaks than Spanish cedar<em> </em>(64% and 25%, respectively). Leaves on elongated shoots dropped off by 20 days after starting the explants in culture and neither the activated charcoal nor the AgNO<sub>3</sub> alone or combined prevented leaf abscission. The AgNO<sub>3</sub> decreased contamination, but also increased leaf abscission. Bud breaks was two-fold higher for nodal explants established in vessels with vented caps than with normal caps. Mahogany<em> </em>nodal explants were easier to surface sterilize and more buds broke from BAP treated explants than Spanish cedar<em> </em>treated explants in the <em style="mso-bidi-font-style: normal;">in vitro</em> establishment.</span></p> <p class="MsoNormal" style="text-align: justify; text-autospace: ideograph-numeric;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US; mso-bidi-font-style: italic;" lang="EN-US">Key words</span></strong><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US">:</span></strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213; mso-ansi-language: EN-US;" lang="EN-US"> Spanish cedar, Mahogany, Mature plants, Surface sterilization, <em>in vitro </em>response</span></p> <p class="MsoNormal" style="text-align: justify; text-autospace: ideograph-numeric;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213;" lang="ES">Resumen: </span></strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213;" lang="ES">La contaminación y la recalcitrancia de tejidos de tallo de Caoba (<em>Swietenia macrophylla</em> King)<em> </em>y Cedro español (<em>Cedrela odorata</em> L.) son las causas principales de su inefectiva micro-propagación. Los objetivos de la investigación fueron: a) </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";;" lang="ES">evaluar el hipoclorito de sodio (<span style="mso-bidi-font-style: italic;">NaClO) y una mezcal preservadora de plantas (PPM</span></span><sup><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: ";Lohit Hindi";;" lang="ES">®</span></sup><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-style: italic;" lang="ES">) como desinfectantes superficiales y/o agregados al medio de cultivo para el establecimiento <em>in vitro</em> de </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213;" lang="ES">explantes nodales de Caoba y Cedro español de 10 años de edad;</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";;" lang="ES"> b) evaluar la respuesta <em style="mso-bidi-font-style: normal;">in vitro</em> de tales explantes tratados con<span style="mso-bidi-font-style: italic;"> </span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213;" lang="ES">N6-benzylaminopurine (BAP) (0, 2.2, 4.4, 8.8, 17.7 μM), nitrato de plata (AgNO<sub>3</sub>) (0, 3 mg l<sup>-1</sup>), carbón activado (0, 1 g l<sup>-1</sup>) y tapas porosas. Los experimentos fueron establecidos bajo un diseño completamente al azar. La contaminación se redujo en ambas especies con NaOCl al 15% durante 20 min como desinfección superficial o con PPM</span><sup><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: Calibri; mso-bidi-font-family: Calibri;" lang="ES">® </span></sup><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: font213;" lang="ES">(2 ml l<sup>-1</sup>) agregado al medio de cultivo. El mayor porcentaje de brotación de explantes se obtuvo con BAP a 17.7 μM en caoba (64%) comparado con cedro (25%). Los brotes se defoliaron a los 20 días de cultivo y ni el carbón activado ni el AgNO<sub>3</sub>, solos o combinados evitaron la defoliación. El AgNO<sub>3</sub> disminuyó la contaminación, pero incrementó la defoliación. La brotación fue dos veces mayor en los explantes nodales establecidos en recipientes con tapas porosas que cuando se utilizaron tapas normales. Los explantes nodales de Caoba respondieron mejor a la desinfección superficial y a los tratamientos de BAP comparados con los de Cedro español en el establecimiento <em>in vitro</em>.</span></p> <p class="MsoNormal" style="text-align: justify; text-autospace: ideograph-numeric;"><strong><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";;" lang="ES">Palabras claves:</span></em></strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";;" lang="ES"> Cedro español, Caoba, Plantas adultas, Desinfección superficial, Respuesta <em>in vitro</em></span></p> |
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| ISSN: | 0123-3475 1909-8758 |