Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch Varieties
In this experiment, 4 varieties of Chinese milk vetch (Xinyang, Minzi No. 6, Minzi 8487711, and Shishou) were used as host plants and inoculated with 3 strains of rhizobium (CCBAU 2609, M. h 93, and 7653R). The differences in their morphology, yield, and nodule characteristics at different growth st...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2020.599400/full |
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| author | Fang Liu Fang Liu Mingxuan Yi Xinbao Liu Yixin Shen Jianlong Li Hui Wang Dianlin Yang Zhengguo Sun |
| author_facet | Fang Liu Fang Liu Mingxuan Yi Xinbao Liu Yixin Shen Jianlong Li Hui Wang Dianlin Yang Zhengguo Sun |
| author_sort | Fang Liu |
| collection | DOAJ |
| description | In this experiment, 4 varieties of Chinese milk vetch (Xinyang, Minzi No. 6, Minzi 8487711, and Shishou) were used as host plants and inoculated with 3 strains of rhizobium (CCBAU 2609, M. h 93, and 7653R). The differences in their morphology, yield, and nodule characteristics at different growth stages were studied and the reasons for these differences were analyzed to explore the exclusivity between different varieties of Chinese milk vetch and strains of rhizobium. Results showed no significant difference in plant height and whole-plant dry weight at seedling stage under different treatments but significant differences in these characteristics at full flowering stage. The results indicated that Minzi 8487711 and Shishou were significantly better than the other varieties. During the whole growth period, the growth indexes and nodule characteristics of the 4 varieties inoculated with strain 7653R were better than those of the varieties inoculated with other strains. At full flowering stage, compared with that of the control group, the biomasses of Xinyang, Minzi No.6, Minzi 8487711, and Shishou had increased by 2.04, 2.84, 1.56, and 2.69-fold, respectively, and nitrogenase activities increased by 3.82, 9.60, 6.21, and 15.18%, respectively. Significant differences in the exclusivity between varieties and strains were observed. Minzi No.6–7653R and Shishou variety–7653R had the strongest exclusivity. The results showed that 7653R was a broad-spectrum and high-efficiency rhizobium strain. 7653R is recommended to be used in combination with Chinese milk vetch varieties, such as Minzi 8487711 and Shishou, to obtain advantages in yield and nitrogen fixation in production. |
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| language | English |
| last_indexed | 2024-12-23T04:22:37Z |
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| spelling | doaj.art-35cb98a80d024f90b7f67a2ceaf026c32022-12-21T18:00:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-12-011110.3389/fpls.2020.599400599400Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch VarietiesFang Liu0Fang Liu1Mingxuan Yi2Xinbao Liu3Yixin Shen4Jianlong Li5Hui Wang6Dianlin Yang7Zhengguo Sun8College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, ChinaSheep Industry Development Center of Yulin City, Yulin, ChinaCollege of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, ChinaCollege of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, ChinaCollege of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, ChinaSchool of Life Sciences, Nanjing University, Nanjing, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Tianjin, ChinaCollege of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, ChinaIn this experiment, 4 varieties of Chinese milk vetch (Xinyang, Minzi No. 6, Minzi 8487711, and Shishou) were used as host plants and inoculated with 3 strains of rhizobium (CCBAU 2609, M. h 93, and 7653R). The differences in their morphology, yield, and nodule characteristics at different growth stages were studied and the reasons for these differences were analyzed to explore the exclusivity between different varieties of Chinese milk vetch and strains of rhizobium. Results showed no significant difference in plant height and whole-plant dry weight at seedling stage under different treatments but significant differences in these characteristics at full flowering stage. The results indicated that Minzi 8487711 and Shishou were significantly better than the other varieties. During the whole growth period, the growth indexes and nodule characteristics of the 4 varieties inoculated with strain 7653R were better than those of the varieties inoculated with other strains. At full flowering stage, compared with that of the control group, the biomasses of Xinyang, Minzi No.6, Minzi 8487711, and Shishou had increased by 2.04, 2.84, 1.56, and 2.69-fold, respectively, and nitrogenase activities increased by 3.82, 9.60, 6.21, and 15.18%, respectively. Significant differences in the exclusivity between varieties and strains were observed. Minzi No.6–7653R and Shishou variety–7653R had the strongest exclusivity. The results showed that 7653R was a broad-spectrum and high-efficiency rhizobium strain. 7653R is recommended to be used in combination with Chinese milk vetch varieties, such as Minzi 8487711 and Shishou, to obtain advantages in yield and nitrogen fixation in production.https://www.frontiersin.org/articles/10.3389/fpls.2020.599400/fullChinese milk vetchrhizobium strainexclusivitybiomassnodule characteristicsnitrogenase activity |
| spellingShingle | Fang Liu Fang Liu Mingxuan Yi Xinbao Liu Yixin Shen Jianlong Li Hui Wang Dianlin Yang Zhengguo Sun Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch Varieties Frontiers in Plant Science Chinese milk vetch rhizobium strain exclusivity biomass nodule characteristics nitrogenase activity |
| title | Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch Varieties |
| title_full | Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch Varieties |
| title_fullStr | Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch Varieties |
| title_full_unstemmed | Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch Varieties |
| title_short | Symbiotic Performances of Three Mesorhizobium huakuii Strains Inoculated to Chinese Milk Vetch Varieties |
| title_sort | symbiotic performances of three mesorhizobium huakuii strains inoculated to chinese milk vetch varieties |
| topic | Chinese milk vetch rhizobium strain exclusivity biomass nodule characteristics nitrogenase activity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2020.599400/full |
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