Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation.
Lack of available iron is one of many environmental challenges that a bacterium encounters during infection and adaptation to iron starvation is important for the pathogen to efficiently replicate within the host. Here we define the transcriptional response of B. anthracis Sterne (34F(2)) to iron de...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-09-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2742718?pdf=render |
| _version_ | 1819034788103716864 |
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| author | Paul E Carlson Katherine A Carr Brian K Janes Erica C Anderson Philip C Hanna |
| author_facet | Paul E Carlson Katherine A Carr Brian K Janes Erica C Anderson Philip C Hanna |
| author_sort | Paul E Carlson |
| collection | DOAJ |
| description | Lack of available iron is one of many environmental challenges that a bacterium encounters during infection and adaptation to iron starvation is important for the pathogen to efficiently replicate within the host. Here we define the transcriptional response of B. anthracis Sterne (34F(2)) to iron depleted conditions. Genome-wide transcript analysis showed that B. anthracis undergoes considerable changes in gene expression during growth in iron-depleted media, including the regulation of known and candidate virulence factors. Two genes encoding putative internalin proteins were chosen for further study. Deletion of either gene (GBAA0552 or GBAA1340) resulted in attenuation in a murine model of infection. This attenuation was amplified in a double mutant strain. These data define the transcriptional changes induced during growth in low iron conditions and illustrate the potential of this dataset in the identification of putative virulence determinants for future study. |
| first_indexed | 2024-12-21T07:39:18Z |
| format | Article |
| id | doaj.art-35d209efed394df8a7aac2dd8cd60ce4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T07:39:18Z |
| publishDate | 2009-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-35d209efed394df8a7aac2dd8cd60ce42022-12-21T19:11:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-09-0149e698810.1371/journal.pone.0006988Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation.Paul E CarlsonKatherine A CarrBrian K JanesErica C AndersonPhilip C HannaLack of available iron is one of many environmental challenges that a bacterium encounters during infection and adaptation to iron starvation is important for the pathogen to efficiently replicate within the host. Here we define the transcriptional response of B. anthracis Sterne (34F(2)) to iron depleted conditions. Genome-wide transcript analysis showed that B. anthracis undergoes considerable changes in gene expression during growth in iron-depleted media, including the regulation of known and candidate virulence factors. Two genes encoding putative internalin proteins were chosen for further study. Deletion of either gene (GBAA0552 or GBAA1340) resulted in attenuation in a murine model of infection. This attenuation was amplified in a double mutant strain. These data define the transcriptional changes induced during growth in low iron conditions and illustrate the potential of this dataset in the identification of putative virulence determinants for future study.http://europepmc.org/articles/PMC2742718?pdf=render |
| spellingShingle | Paul E Carlson Katherine A Carr Brian K Janes Erica C Anderson Philip C Hanna Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation. PLoS ONE |
| title | Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation. |
| title_full | Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation. |
| title_fullStr | Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation. |
| title_full_unstemmed | Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation. |
| title_short | Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation. |
| title_sort | transcriptional profiling of bacillus anthracis sterne 34f2 during iron starvation |
| url | http://europepmc.org/articles/PMC2742718?pdf=render |
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