Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum
To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respective...
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Energy Research |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fenrg.2018.00001/full |
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| author | Lan Yao Lan Yao Lan Yao Haitao Yang Haitao Yang Chang Geun Yoo Xianzhi Meng Yunqiao Pu Naijia Hao Arthur J. Ragauskas Arthur J. Ragauskas Arthur J. Ragauskas |
| author_facet | Lan Yao Lan Yao Lan Yao Haitao Yang Haitao Yang Chang Geun Yoo Xianzhi Meng Yunqiao Pu Naijia Hao Arthur J. Ragauskas Arthur J. Ragauskas Arthur J. Ragauskas |
| author_sort | Lan Yao |
| collection | DOAJ |
| description | To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength. |
| first_indexed | 2024-12-12T08:25:45Z |
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| id | doaj.art-35d25ac5557d478699c2c425b9223d23 |
| institution | Directory Open Access Journal |
| issn | 2296-598X |
| language | English |
| last_indexed | 2024-12-12T08:25:45Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Energy Research |
| spelling | doaj.art-35d25ac5557d478699c2c425b9223d232022-12-22T00:31:15ZengFrontiers Media S.A.Frontiers in Energy Research2296-598X2018-02-01610.3389/fenrg.2018.00001336502Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatumLan Yao0Lan Yao1Lan Yao2Haitao Yang3Haitao Yang4Chang Geun Yoo5Xianzhi Meng6Yunqiao Pu7Naijia Hao8Arthur J. Ragauskas9Arthur J. Ragauskas10Arthur J. Ragauskas11Hubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, Wuhan, ChinaState Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, ChinaDepartment of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN, United StatesHubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, Wuhan, ChinaState Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, ChinaJoint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN, United StatesDepartment of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN, United StatesDepartment of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN, United StatesDepartment of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN, United StatesDepartment of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN, United StatesJoint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN, United StatesDepartment of Forestry, Wildlife and Fisheries, Center for Renewable Carbon, Institute of Agriculture, The University of Tennessee, Knoxville, TN, United StatesTo investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.http://journal.frontiersin.org/article/10.3389/fenrg.2018.00001/fullcharacterizationlignindilute acid pretreatmentswitchgrasscellobiohydrolase |
| spellingShingle | Lan Yao Lan Yao Lan Yao Haitao Yang Haitao Yang Chang Geun Yoo Xianzhi Meng Yunqiao Pu Naijia Hao Arthur J. Ragauskas Arthur J. Ragauskas Arthur J. Ragauskas Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum Frontiers in Energy Research characterization lignin dilute acid pretreatment switchgrass cellobiohydrolase |
| title | Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum |
| title_full | Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum |
| title_fullStr | Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum |
| title_full_unstemmed | Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum |
| title_short | Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum |
| title_sort | characteristics of lignin fractions from dilute acid pretreated switchgrass and their effect on cellobiohydrolase from trichoderma longibrachiatum |
| topic | characterization lignin dilute acid pretreatment switchgrass cellobiohydrolase |
| url | http://journal.frontiersin.org/article/10.3389/fenrg.2018.00001/full |
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