Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 Pathway
In this study, we investigate the ameliorating functions of QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) from monkfish swim bladders on an FFA-induced NAFLD model of HepG2 cells. The lipid-lowering mechanisms revealed that these five oligopeptides can up-regulate the expre...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/21/6/360 |
| _version_ | 1797593706256138240 |
|---|---|
| author | Ming-Feng Wu Qing-Hao Xi Yan Sheng Yu-Mei Wang Wan-Yi Wang Chang-Feng Chi Bin Wang |
| author_facet | Ming-Feng Wu Qing-Hao Xi Yan Sheng Yu-Mei Wang Wan-Yi Wang Chang-Feng Chi Bin Wang |
| author_sort | Ming-Feng Wu |
| collection | DOAJ |
| description | In this study, we investigate the ameliorating functions of QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) from monkfish swim bladders on an FFA-induced NAFLD model of HepG2 cells. The lipid-lowering mechanisms revealed that these five oligopeptides can up-regulate the expression of phospho-AMP-activated protein kinase (<i>p</i>-AMPK) proteins to inhibit the expression of the sterol regulatory element binding protein-1c (SREBP-1c) proteins on increasing lipid synthesis and up-regulating the expression of the PPAP-α and CPT-1 proteins on promoting the β-oxidation of fatty acids. Moreover, QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) can significantly inhibit reactive oxygen species’ (ROS) production, promote the activities of intracellular antioxidases (superoxide dismutase, SOD; glutathione peroxidase, GSH-PX; and catalase, CAT) and bring down the content of malondialdehyde (MDA) derived from lipid peroxidation. Further investigations revealed that the regulation of these five oligopeptides on oxidative stress was achieved through activating the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway to raise the expression levels of the heme oxygenase 1 (HO-1) protein and downstream antioxidant proteases. Therefore, QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) could serve as candidate ingredients to develop functional products for treating NAFLD. |
| first_indexed | 2024-03-11T02:13:17Z |
| format | Article |
| id | doaj.art-35d2afc8d65f491ba3d144a06de11854 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T02:13:17Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-35d2afc8d65f491ba3d144a06de118542023-11-18T11:22:49ZengMDPI AGMarine Drugs1660-33972023-06-0121636010.3390/md21060360Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 PathwayMing-Feng Wu0Qing-Hao Xi1Yan Sheng2Yu-Mei Wang3Wan-Yi Wang4Chang-Feng Chi5Bin Wang6Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaIn this study, we investigate the ameliorating functions of QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) from monkfish swim bladders on an FFA-induced NAFLD model of HepG2 cells. The lipid-lowering mechanisms revealed that these five oligopeptides can up-regulate the expression of phospho-AMP-activated protein kinase (<i>p</i>-AMPK) proteins to inhibit the expression of the sterol regulatory element binding protein-1c (SREBP-1c) proteins on increasing lipid synthesis and up-regulating the expression of the PPAP-α and CPT-1 proteins on promoting the β-oxidation of fatty acids. Moreover, QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) can significantly inhibit reactive oxygen species’ (ROS) production, promote the activities of intracellular antioxidases (superoxide dismutase, SOD; glutathione peroxidase, GSH-PX; and catalase, CAT) and bring down the content of malondialdehyde (MDA) derived from lipid peroxidation. Further investigations revealed that the regulation of these five oligopeptides on oxidative stress was achieved through activating the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway to raise the expression levels of the heme oxygenase 1 (HO-1) protein and downstream antioxidant proteases. Therefore, QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) could serve as candidate ingredients to develop functional products for treating NAFLD.https://www.mdpi.com/1660-3397/21/6/360monkfish (<i>Lophius litulon</i>)swim bladdersantioxidant peptidenonalcoholic fatty liver disease (NAFLD)AMPK/Nrf2 pathway |
| spellingShingle | Ming-Feng Wu Qing-Hao Xi Yan Sheng Yu-Mei Wang Wan-Yi Wang Chang-Feng Chi Bin Wang Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 Pathway Marine Drugs monkfish (<i>Lophius litulon</i>) swim bladders antioxidant peptide nonalcoholic fatty liver disease (NAFLD) AMPK/Nrf2 pathway |
| title | Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 Pathway |
| title_full | Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 Pathway |
| title_fullStr | Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 Pathway |
| title_full_unstemmed | Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 Pathway |
| title_short | Antioxidant Peptides from Monkfish Swim Bladders: Ameliorating NAFLD In Vitro by Suppressing Lipid Accumulation and Oxidative Stress via Regulating AMPK/Nrf2 Pathway |
| title_sort | antioxidant peptides from monkfish swim bladders ameliorating nafld in vitro by suppressing lipid accumulation and oxidative stress via regulating ampk nrf2 pathway |
| topic | monkfish (<i>Lophius litulon</i>) swim bladders antioxidant peptide nonalcoholic fatty liver disease (NAFLD) AMPK/Nrf2 pathway |
| url | https://www.mdpi.com/1660-3397/21/6/360 |
| work_keys_str_mv | AT mingfengwu antioxidantpeptidesfrommonkfishswimbladdersamelioratingnafldinvitrobysuppressinglipidaccumulationandoxidativestressviaregulatingampknrf2pathway AT qinghaoxi antioxidantpeptidesfrommonkfishswimbladdersamelioratingnafldinvitrobysuppressinglipidaccumulationandoxidativestressviaregulatingampknrf2pathway AT yansheng antioxidantpeptidesfrommonkfishswimbladdersamelioratingnafldinvitrobysuppressinglipidaccumulationandoxidativestressviaregulatingampknrf2pathway AT yumeiwang antioxidantpeptidesfrommonkfishswimbladdersamelioratingnafldinvitrobysuppressinglipidaccumulationandoxidativestressviaregulatingampknrf2pathway AT wanyiwang antioxidantpeptidesfrommonkfishswimbladdersamelioratingnafldinvitrobysuppressinglipidaccumulationandoxidativestressviaregulatingampknrf2pathway AT changfengchi antioxidantpeptidesfrommonkfishswimbladdersamelioratingnafldinvitrobysuppressinglipidaccumulationandoxidativestressviaregulatingampknrf2pathway AT binwang antioxidantpeptidesfrommonkfishswimbladdersamelioratingnafldinvitrobysuppressinglipidaccumulationandoxidativestressviaregulatingampknrf2pathway |