Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh
Abstract Background Due to its rapid lethal effect in the early development stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) has been causing great economic losses, since its first outbreak in southeast China in 2009. Vibrio parahaemolyticus, carrying the pirA and pirB toxin genes is...
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-019-1655-8 |
| _version_ | 1818730653637672960 |
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| author | Shawon Ahmmed Md. Abdullah-Al-Kamran Khan Md. Mostavi Enan Eshik Nusrat Jahan Punom Abul Bashar Mir Md. Khademul Islam Mohammad Shamsur Rahman |
| author_facet | Shawon Ahmmed Md. Abdullah-Al-Kamran Khan Md. Mostavi Enan Eshik Nusrat Jahan Punom Abul Bashar Mir Md. Khademul Islam Mohammad Shamsur Rahman |
| author_sort | Shawon Ahmmed |
| collection | DOAJ |
| description | Abstract Background Due to its rapid lethal effect in the early development stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) has been causing great economic losses, since its first outbreak in southeast China in 2009. Vibrio parahaemolyticus, carrying the pirA and pirB toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence the whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp (Peneaus monodon) of the south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh, AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of a total of 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type III secretion system-1 (T3SS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~ 69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pirA and pirB. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline and macrolide groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic-resistant V. parahaemolyticus isolates between production sites, and the identification of candidate strains for the production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world. |
| first_indexed | 2024-12-17T23:05:12Z |
| format | Article |
| id | doaj.art-35d3360631af439e8ac3d87d425c85fd |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-17T23:05:12Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-35d3360631af439e8ac3d87d425c85fd2022-12-21T21:29:18ZengBMCBMC Microbiology1471-21802019-12-0119111410.1186/s12866-019-1655-8Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west BangladeshShawon Ahmmed0Md. Abdullah-Al-Kamran Khan1Md. Mostavi Enan Eshik2Nusrat Jahan Punom3Abul Bashar Mir Md. Khademul Islam4Mohammad Shamsur Rahman5Aquatic Animal Health Group, Department of Fisheries, University of DhakaDepartment of Genetic Engineering and Biotechnology, University of DhakaAquatic Animal Health Group, Department of Fisheries, University of DhakaAquatic Animal Health Group, Department of Fisheries, University of DhakaDepartment of Genetic Engineering and Biotechnology, University of DhakaAquatic Animal Health Group, Department of Fisheries, University of DhakaAbstract Background Due to its rapid lethal effect in the early development stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) has been causing great economic losses, since its first outbreak in southeast China in 2009. Vibrio parahaemolyticus, carrying the pirA and pirB toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence the whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp (Peneaus monodon) of the south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh, AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of a total of 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type III secretion system-1 (T3SS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~ 69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pirA and pirB. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline and macrolide groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic-resistant V. parahaemolyticus isolates between production sites, and the identification of candidate strains for the production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world.https://doi.org/10.1186/s12866-019-1655-8Vibrio parahaemolyticusAcute hepatopancreatic necrosis diseaseAHPNDGenome sequencingVirulencepirA |
| spellingShingle | Shawon Ahmmed Md. Abdullah-Al-Kamran Khan Md. Mostavi Enan Eshik Nusrat Jahan Punom Abul Bashar Mir Md. Khademul Islam Mohammad Shamsur Rahman Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh BMC Microbiology Vibrio parahaemolyticus Acute hepatopancreatic necrosis disease AHPND Genome sequencing Virulence pirA |
| title | Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh |
| title_full | Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh |
| title_fullStr | Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh |
| title_full_unstemmed | Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh |
| title_short | Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh |
| title_sort | genomic and evolutionary features of two ahpnd positive vibrio parahaemolyticus strains isolated from shrimp penaeus monodon of south west bangladesh |
| topic | Vibrio parahaemolyticus Acute hepatopancreatic necrosis disease AHPND Genome sequencing Virulence pirA |
| url | https://doi.org/10.1186/s12866-019-1655-8 |
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