Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells
Phototherapy using narrowband ultraviolet-B (NB-UVB) has been shown to be more effective than conventional broadband UVB (BB-UVB) in treating a variety of skin diseases. To assess the difference in carcinogenic potential between NB-UVB and BB-UVB, we investigated the cytotoxicity via colony formatio...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/6/646 |
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| author | Dylan J. Buglewicz Jacob T. Mussallem Alexis H. Haskins Cathy Su Junko Maeda Takamitsu A. Kato |
| author_facet | Dylan J. Buglewicz Jacob T. Mussallem Alexis H. Haskins Cathy Su Junko Maeda Takamitsu A. Kato |
| author_sort | Dylan J. Buglewicz |
| collection | DOAJ |
| description | Phototherapy using narrowband ultraviolet-B (NB-UVB) has been shown to be more effective than conventional broadband UVB (BB-UVB) in treating a variety of skin diseases. To assess the difference in carcinogenic potential between NB-UVB and BB-UVB, we investigated the cytotoxicity via colony formation assay, genotoxicity via sister chromatid exchange (SCE) assay, mutagenicity via hypoxanthine phosphoribosyltransferase (HPRT) mutation assay, as well as cyclobutane pyrimidine dimer (CPD) formation and reactive oxygen species (ROS) generation in Chinese hamster ovary (CHO) and their NER mutant cells. The radiation dose required to reduce survival to 10% (D<sub>10</sub> value) demonstrated BB-UVB was 10 times more cytotoxic than NB-UVB, and revealed that NB-UVB also induces DNA damage repaired by nucleotide excision repair. We also found that BB-UVB more efficiently induced SCEs and HPRT mutations per absorbed energy dosage (J/m<sup>2</sup>) than NB-UVB. However, SCE and HPRT mutation frequencies were observed to rise in noncytotoxic dosages of NB-UVB exposure. BB-UVB and NB-UVB both produced a significant increase in CPD formation and ROS formation (<i>p</i> < 0.05); however, higher dosages were required for NB-UVB. These results suggest that NB-UVB is less cytotoxic and genotoxic than BB-UVB, but can still produce genotoxic effects even at noncytotoxic doses. |
| first_indexed | 2024-03-09T06:13:29Z |
| format | Article |
| id | doaj.art-35d4e061cf5d43f5ab3c56a7ed81e23a |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T06:13:29Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-35d4e061cf5d43f5ab3c56a7ed81e23a2023-12-03T11:56:09ZengMDPI AGGenes2073-44252020-06-0111664610.3390/genes11060646Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian CellsDylan J. Buglewicz0Jacob T. Mussallem1Alexis H. Haskins2Cathy Su3Junko Maeda4Takamitsu A. Kato5Department of Environmental & Radiological Health Sciences, Colorado State University, 1618 Campus Delivery, Fort Collins, CO 80523, USADepartment of Environmental & Radiological Health Sciences, Colorado State University, 1618 Campus Delivery, Fort Collins, CO 80523, USADepartment of Environmental & Radiological Health Sciences, Colorado State University, 1618 Campus Delivery, Fort Collins, CO 80523, USADepartment of Environmental & Radiological Health Sciences, Colorado State University, 1618 Campus Delivery, Fort Collins, CO 80523, USADepartment of Environmental & Radiological Health Sciences, Colorado State University, 1618 Campus Delivery, Fort Collins, CO 80523, USADepartment of Environmental & Radiological Health Sciences, Colorado State University, 1618 Campus Delivery, Fort Collins, CO 80523, USAPhototherapy using narrowband ultraviolet-B (NB-UVB) has been shown to be more effective than conventional broadband UVB (BB-UVB) in treating a variety of skin diseases. To assess the difference in carcinogenic potential between NB-UVB and BB-UVB, we investigated the cytotoxicity via colony formation assay, genotoxicity via sister chromatid exchange (SCE) assay, mutagenicity via hypoxanthine phosphoribosyltransferase (HPRT) mutation assay, as well as cyclobutane pyrimidine dimer (CPD) formation and reactive oxygen species (ROS) generation in Chinese hamster ovary (CHO) and their NER mutant cells. The radiation dose required to reduce survival to 10% (D<sub>10</sub> value) demonstrated BB-UVB was 10 times more cytotoxic than NB-UVB, and revealed that NB-UVB also induces DNA damage repaired by nucleotide excision repair. We also found that BB-UVB more efficiently induced SCEs and HPRT mutations per absorbed energy dosage (J/m<sup>2</sup>) than NB-UVB. However, SCE and HPRT mutation frequencies were observed to rise in noncytotoxic dosages of NB-UVB exposure. BB-UVB and NB-UVB both produced a significant increase in CPD formation and ROS formation (<i>p</i> < 0.05); however, higher dosages were required for NB-UVB. These results suggest that NB-UVB is less cytotoxic and genotoxic than BB-UVB, but can still produce genotoxic effects even at noncytotoxic doses.https://www.mdpi.com/2073-4425/11/6/646narrowband UVBbroadband UVBcytotoxicityHPRTSCEDNA damage |
| spellingShingle | Dylan J. Buglewicz Jacob T. Mussallem Alexis H. Haskins Cathy Su Junko Maeda Takamitsu A. Kato Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells Genes narrowband UVB broadband UVB cytotoxicity HPRT SCE DNA damage |
| title | Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells |
| title_full | Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells |
| title_fullStr | Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells |
| title_full_unstemmed | Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells |
| title_short | Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells |
| title_sort | cytotoxicity and mutagenicity of narrowband uvb to mammalian cells |
| topic | narrowband UVB broadband UVB cytotoxicity HPRT SCE DNA damage |
| url | https://www.mdpi.com/2073-4425/11/6/646 |
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