An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1.
Heterochromatin formation involves the nucleation and spreading of structural and epigenetic features along the chromatin fiber. Chromatin barriers and associated proteins counteract the spreading of heterochromatin, thereby restricting it to specific regions of the genome. We have performed gene ex...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2007-10-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2040200?pdf=render |
| _version_ | 1818863441419436032 |
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| author | Kristin C Scott Caroline V White Huntington F Willard |
| author_facet | Kristin C Scott Caroline V White Huntington F Willard |
| author_sort | Kristin C Scott |
| collection | DOAJ |
| description | Heterochromatin formation involves the nucleation and spreading of structural and epigenetic features along the chromatin fiber. Chromatin barriers and associated proteins counteract the spreading of heterochromatin, thereby restricting it to specific regions of the genome. We have performed gene expression studies and chromatin immunoprecipitation on strains in which native centromere sequences have been mutated to study the mechanism by which a tRNA(Alanine) gene barrier (cen1 tDNA(Ala)) blocks the spread of pericentromeric heterochromatin at the centromere of chromosome 1 (cen1) in the fission yeast, Schizosaccharomyces pombe. Within the centromere, barrier activity is a general property of tDNAs and, unlike previously characterized barriers, requires the association of both transcription factor IIIC and RNA Polymerase III. Although the cen1 tDNA(Ala) gene is actively transcribed, barrier activity is independent of transcriptional orientation. These findings provide experimental evidence for the involvement of a fully assembled RNA polymerase III transcription complex in defining independent structural and functional domains at a eukaryotic centromere. |
| first_indexed | 2024-12-19T10:15:49Z |
| format | Article |
| id | doaj.art-35d6b2551708413dbcd8c525d00acf19 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T10:15:49Z |
| publishDate | 2007-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-35d6b2551708413dbcd8c525d00acf192022-12-21T20:26:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-10-01210e109910.1371/journal.pone.0001099An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1.Kristin C ScottCaroline V WhiteHuntington F WillardHeterochromatin formation involves the nucleation and spreading of structural and epigenetic features along the chromatin fiber. Chromatin barriers and associated proteins counteract the spreading of heterochromatin, thereby restricting it to specific regions of the genome. We have performed gene expression studies and chromatin immunoprecipitation on strains in which native centromere sequences have been mutated to study the mechanism by which a tRNA(Alanine) gene barrier (cen1 tDNA(Ala)) blocks the spread of pericentromeric heterochromatin at the centromere of chromosome 1 (cen1) in the fission yeast, Schizosaccharomyces pombe. Within the centromere, barrier activity is a general property of tDNAs and, unlike previously characterized barriers, requires the association of both transcription factor IIIC and RNA Polymerase III. Although the cen1 tDNA(Ala) gene is actively transcribed, barrier activity is independent of transcriptional orientation. These findings provide experimental evidence for the involvement of a fully assembled RNA polymerase III transcription complex in defining independent structural and functional domains at a eukaryotic centromere.http://europepmc.org/articles/PMC2040200?pdf=render |
| spellingShingle | Kristin C Scott Caroline V White Huntington F Willard An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1. PLoS ONE |
| title | An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1. |
| title_full | An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1. |
| title_fullStr | An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1. |
| title_full_unstemmed | An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1. |
| title_short | An RNA polymerase III-dependent heterochromatin barrier at fission yeast centromere 1. |
| title_sort | rna polymerase iii dependent heterochromatin barrier at fission yeast centromere 1 |
| url | http://europepmc.org/articles/PMC2040200?pdf=render |
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