Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm.
BACKGROUND:Atrial fibrillation (AF) is the most common arrhythmia and despite obvious clinical importance remains its pathogenesis only partially explained. A relation between inflammation and AF has been suggested by findings of increased inflammatory markers in AF patients. OBJECTIVE:The goal of t...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5321459?pdf=render |
| _version_ | 1818285419929796608 |
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| author | Natalia Smorodinova Martin Bláha Vojtěch Melenovský Karolína Rozsívalová Jaromír Přidal Mária Ďurišová Jan Pirk Josef Kautzner Tomáš Kučera |
| author_facet | Natalia Smorodinova Martin Bláha Vojtěch Melenovský Karolína Rozsívalová Jaromír Přidal Mária Ďurišová Jan Pirk Josef Kautzner Tomáš Kučera |
| author_sort | Natalia Smorodinova |
| collection | DOAJ |
| description | BACKGROUND:Atrial fibrillation (AF) is the most common arrhythmia and despite obvious clinical importance remains its pathogenesis only partially explained. A relation between inflammation and AF has been suggested by findings of increased inflammatory markers in AF patients. OBJECTIVE:The goal of this study was to characterize morphologically and functionally CD45-positive inflammatory cell populations in atrial myocardium of patients with AF as compared to sinus rhythm (SR). METHODS:We examined 46 subjects (19 with AF, and 27 in SR) undergoing coronary bypass or valve surgery. Peroperative bioptic samples of the left and the right atrial tissue were examined using immunohistochemistry. RESULTS:The number of CD3+ T-lymphocytes and CD68-KP1+ cells were elevated in the left atrial myocardium of patients with AF compared to those in SR. Immune cell infiltration of LA was related to the rhythm, but not to age, body size, LA size, mitral regurgitation grade, type of surgery, systemic markers of inflammation or presence of diabetes or hypertension. Most of CD68-KP1+ cells corresponded to dendritic cell population based on their morphology and immunoreactivity for DC-SIGN. The numbers of mast cells and CD20+ B-lymphocytes did not differ between AF and SR patients. No foci of inflammation were detected in any sample. CONCLUSIONS:An immunohistochemical analysis of samples from patients undergoing open heart surgery showed moderate and site-specific increase of inflammatory cells in the atrial myocardium of patients with AF compared to those in SR, with prevailing population of monocyte-macrophage lineage. These cells and their cytokine products may play a role in atrial remodeling and AF persistence. |
| first_indexed | 2024-12-13T01:08:24Z |
| format | Article |
| id | doaj.art-35d7a6521ed445ad8023d33b8658360e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T01:08:24Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-35d7a6521ed445ad8023d33b8658360e2022-12-22T00:04:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017269110.1371/journal.pone.0172691Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm.Natalia SmorodinovaMartin BláhaVojtěch MelenovskýKarolína RozsívalováJaromír PřidalMária ĎurišováJan PirkJosef KautznerTomáš KučeraBACKGROUND:Atrial fibrillation (AF) is the most common arrhythmia and despite obvious clinical importance remains its pathogenesis only partially explained. A relation between inflammation and AF has been suggested by findings of increased inflammatory markers in AF patients. OBJECTIVE:The goal of this study was to characterize morphologically and functionally CD45-positive inflammatory cell populations in atrial myocardium of patients with AF as compared to sinus rhythm (SR). METHODS:We examined 46 subjects (19 with AF, and 27 in SR) undergoing coronary bypass or valve surgery. Peroperative bioptic samples of the left and the right atrial tissue were examined using immunohistochemistry. RESULTS:The number of CD3+ T-lymphocytes and CD68-KP1+ cells were elevated in the left atrial myocardium of patients with AF compared to those in SR. Immune cell infiltration of LA was related to the rhythm, but not to age, body size, LA size, mitral regurgitation grade, type of surgery, systemic markers of inflammation or presence of diabetes or hypertension. Most of CD68-KP1+ cells corresponded to dendritic cell population based on their morphology and immunoreactivity for DC-SIGN. The numbers of mast cells and CD20+ B-lymphocytes did not differ between AF and SR patients. No foci of inflammation were detected in any sample. CONCLUSIONS:An immunohistochemical analysis of samples from patients undergoing open heart surgery showed moderate and site-specific increase of inflammatory cells in the atrial myocardium of patients with AF compared to those in SR, with prevailing population of monocyte-macrophage lineage. These cells and their cytokine products may play a role in atrial remodeling and AF persistence.http://europepmc.org/articles/PMC5321459?pdf=render |
| spellingShingle | Natalia Smorodinova Martin Bláha Vojtěch Melenovský Karolína Rozsívalová Jaromír Přidal Mária Ďurišová Jan Pirk Josef Kautzner Tomáš Kučera Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm. PLoS ONE |
| title | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm. |
| title_full | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm. |
| title_fullStr | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm. |
| title_full_unstemmed | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm. |
| title_short | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm. |
| title_sort | analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm |
| url | http://europepmc.org/articles/PMC5321459?pdf=render |
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