Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil
ABSTRACT Objective: Nontuberculous mycobacteria (NTM) are a heterogeneous group of bacteria that are widely distributed in nature and associated with opportunistic infections in humans. The aims of this study were to identify NTM in patients with suspected tuberculosis who presented positive cultur...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pneumologia e Tisiologia
2020-05-01
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| Series: | Jornal Brasileiro de Pneumologia |
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| Online Access: | http://www.scielo.br/pdf/jbpneu/v46n2/1806-3713-jbpneu-46-02-e20190184.pdf |
| _version_ | 1818752951008624640 |
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| author | Caroline Busatto Júlia Silveira Vianna Ana Barbara Scholante Silva Rossana Basso Jussara Silveira Andrea Von Groll Ivy Bastos Ramis Pedro Eduardo Almeida da Silva |
| author_facet | Caroline Busatto Júlia Silveira Vianna Ana Barbara Scholante Silva Rossana Basso Jussara Silveira Andrea Von Groll Ivy Bastos Ramis Pedro Eduardo Almeida da Silva |
| author_sort | Caroline Busatto |
| collection | DOAJ |
| description | ABSTRACT Objective: Nontuberculous mycobacteria (NTM) are a heterogeneous group of bacteria that are widely distributed in nature and associated with opportunistic infections in humans. The aims of this study were to identify NTM in patients with suspected tuberculosis who presented positive cultures and to evaluate the genetic diversity of strains identified as Mycobacterium avium. Methods: We studied pulmonary and extrapulmonary samples obtained from 1,248 patients. The samples that tested positive on culture and negative for the M. tuberculosis complex by molecular identification techniques were evaluated by detection of the hsp65 and rpoB genes and sequencing of conserved fragments of these genes. All strains identified as M. avium were genotyped using the eight-locus mycobacterial interspersed repetitive unit-variable-number tandem-repeat method. Results: We found that NTM accounted for 25 (7.5%) of the 332 mycobacteria isolated. Of those 25, 18 (72%) were M. avium, 5 (20%) were M. abscessus, 1 (4%) was M. gastri, and 1 (4%) was M. kansasii. The 18 M. avium strains showed high diversity, only two strains being genetically related. Conclusions: These results highlight the need to consider the investigation of NTM in patients with suspected active tuberculosis who present with positive cultures, as well as to evaluate the genetic diversity of M. avium strains. |
| first_indexed | 2024-12-18T04:59:37Z |
| format | Article |
| id | doaj.art-35dce576df184edab4adf86370cf15ad |
| institution | Directory Open Access Journal |
| issn | 1806-3756 |
| language | English |
| last_indexed | 2024-12-18T04:59:37Z |
| publishDate | 2020-05-01 |
| publisher | Sociedade Brasileira de Pneumologia e Tisiologia |
| record_format | Article |
| series | Jornal Brasileiro de Pneumologia |
| spelling | doaj.art-35dce576df184edab4adf86370cf15ad2022-12-21T21:20:10ZengSociedade Brasileira de Pneumologia e TisiologiaJornal Brasileiro de Pneumologia1806-37562020-05-0146210.36416/1806-3756/e20190184Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of BrazilCaroline Busattohttps://orcid.org/0000-0003-3463-9390Júlia Silveira Viannahttps://orcid.org/0000-0002-4550-9148Ana Barbara Scholante Silvahttps://orcid.org/0000-0001-6816-2055Rossana Bassohttps://orcid.org/0000-0001-9317-8219Jussara Silveirahttps://orcid.org/0000-0003-1652-2447Andrea Von Grollhttps://orcid.org/0000-0002-6727-372XIvy Bastos Ramishttps://orcid.org/0000-0003-2283-5087Pedro Eduardo Almeida da Silvahttps://orcid.org/0000-0003-1666-1295ABSTRACT Objective: Nontuberculous mycobacteria (NTM) are a heterogeneous group of bacteria that are widely distributed in nature and associated with opportunistic infections in humans. The aims of this study were to identify NTM in patients with suspected tuberculosis who presented positive cultures and to evaluate the genetic diversity of strains identified as Mycobacterium avium. Methods: We studied pulmonary and extrapulmonary samples obtained from 1,248 patients. The samples that tested positive on culture and negative for the M. tuberculosis complex by molecular identification techniques were evaluated by detection of the hsp65 and rpoB genes and sequencing of conserved fragments of these genes. All strains identified as M. avium were genotyped using the eight-locus mycobacterial interspersed repetitive unit-variable-number tandem-repeat method. Results: We found that NTM accounted for 25 (7.5%) of the 332 mycobacteria isolated. Of those 25, 18 (72%) were M. avium, 5 (20%) were M. abscessus, 1 (4%) was M. gastri, and 1 (4%) was M. kansasii. The 18 M. avium strains showed high diversity, only two strains being genetically related. Conclusions: These results highlight the need to consider the investigation of NTM in patients with suspected active tuberculosis who present with positive cultures, as well as to evaluate the genetic diversity of M. avium strains.http://www.scielo.br/pdf/jbpneu/v46n2/1806-3713-jbpneu-46-02-e20190184.pdfMycobacterium aviumNontuberculous mycobacteriaHIVGenotyping techniques |
| spellingShingle | Caroline Busatto Júlia Silveira Vianna Ana Barbara Scholante Silva Rossana Basso Jussara Silveira Andrea Von Groll Ivy Bastos Ramis Pedro Eduardo Almeida da Silva Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil Jornal Brasileiro de Pneumologia Mycobacterium avium Nontuberculous mycobacteria HIV Genotyping techniques |
| title | Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil |
| title_full | Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil |
| title_fullStr | Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil |
| title_full_unstemmed | Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil |
| title_short | Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil |
| title_sort | nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of mycobacterium avium in the extreme south of brazil |
| topic | Mycobacterium avium Nontuberculous mycobacteria HIV Genotyping techniques |
| url | http://www.scielo.br/pdf/jbpneu/v46n2/1806-3713-jbpneu-46-02-e20190184.pdf |
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