The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1α C1772T Polymorphism as Predictor of Laryngeal Carcinoma Relapse
Introduction The association between the expression of HIF-1α in the laryngeal carcinoma and the prognosis of disease is quite well documented, but the significance of HIF-1α C1772T polymorphism and its relation to disease phenotype have to be clarified. The aim of this study was to investigate the...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2022-12-01
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| Series: | Cancer Control |
| Online Access: | https://doi.org/10.1177/10732748221144457 |
| _version_ | 1811311593179316224 |
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| author | Miljan M. Folic MD, PhD Ana V. Banko MD, PhD Vera N. Todorovic MD, PhD Nela S. Puskas MD, PhD Jovica P. Milovanovic MD, PhD Sanja B. Krejovic MD, PhD Neda Z. Dragicevic-Babic MD Zoran M. Bukumiric MD, PhD Biljana R. Milicic MD, PhD Ana D. Jotic MD, PhD Vojko B. Djukic MD, PhD |
| author_facet | Miljan M. Folic MD, PhD Ana V. Banko MD, PhD Vera N. Todorovic MD, PhD Nela S. Puskas MD, PhD Jovica P. Milovanovic MD, PhD Sanja B. Krejovic MD, PhD Neda Z. Dragicevic-Babic MD Zoran M. Bukumiric MD, PhD Biljana R. Milicic MD, PhD Ana D. Jotic MD, PhD Vojko B. Djukic MD, PhD |
| author_sort | Miljan M. Folic MD, PhD |
| collection | DOAJ |
| description | Introduction The association between the expression of HIF-1α in the laryngeal carcinoma and the prognosis of disease is quite well documented, but the significance of HIF-1α C1772T polymorphism and its relation to disease phenotype have to be clarified. The aim of this study was to investigate the influence of C1772T polymorphism on the clinical-pathological characteristics and disease-free survival after initial surgical treatment of patients with laryngeal carcinoma. Materials and methods The prospective cohort study included 65 patients with laryngeal carcinoma. Two representative tumor tissue specimens were taken in each patient during surgery; 1 specimen was used to asses HIF-1α C1772T polymorphism and the other 1 to determine the immunohistochemical expression of HIF-1α, VEGF, as well as CD 34 proteins. The comparison of polymorphism frequency between study and control population was conducted by collecting a 5 mL of peripheral venous blood samples in each subject. Results Clinicopathological characteristics of laryngeal carcinoma didn’t affect the expression of hypoxia-related biomarkers, such as HIF-1α, VEGF or MVD. The statistically significant association between HIF-1α and VEGF expression was found ( P = .034), but not between HIF-1α expression and MVD value ( P = .696). The expression of HIF-1α was significantly higher among CT heterozygotes ( P = .029). We found a significantly more recurrence among CT heterozygotes compared with patients with CC homozygous alleles (57.10% and 24.30%, respectively; P = .007). Patients with C1772T polymorphic variants had significantly worse disease-free survival compared with patients without polymorphism (Log-rank test, P = .007). Conclusion HIF-1α C1772T polymorphism was significantly associated with worse disease-free survival which nominates it as a predictor of laryngeal carcinoma relapse. The preoperative assessment of hypoxia-related biomarkers should be used in everyday practice in order to determine the treatment modalities for laryngeal carcinoma. |
| first_indexed | 2024-04-13T10:21:35Z |
| format | Article |
| id | doaj.art-35ddf1ea3b264accaededd1ba2961062 |
| institution | Directory Open Access Journal |
| issn | 1073-2748 |
| language | English |
| last_indexed | 2024-04-13T10:21:35Z |
| publishDate | 2022-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cancer Control |
| spelling | doaj.art-35ddf1ea3b264accaededd1ba29610622022-12-22T02:50:28ZengSAGE PublishingCancer Control1073-27482022-12-012910.1177/10732748221144457The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1α C1772T Polymorphism as Predictor of Laryngeal Carcinoma RelapseMiljan M. Folic MD, PhDAna V. Banko MD, PhDVera N. Todorovic MD, PhDNela S. Puskas MD, PhDJovica P. Milovanovic MD, PhDSanja B. Krejovic MD, PhDNeda Z. Dragicevic-Babic MDZoran M. Bukumiric MD, PhDBiljana R. Milicic MD, PhDAna D. Jotic MD, PhDVojko B. Djukic MD, PhDIntroduction The association between the expression of HIF-1α in the laryngeal carcinoma and the prognosis of disease is quite well documented, but the significance of HIF-1α C1772T polymorphism and its relation to disease phenotype have to be clarified. The aim of this study was to investigate the influence of C1772T polymorphism on the clinical-pathological characteristics and disease-free survival after initial surgical treatment of patients with laryngeal carcinoma. Materials and methods The prospective cohort study included 65 patients with laryngeal carcinoma. Two representative tumor tissue specimens were taken in each patient during surgery; 1 specimen was used to asses HIF-1α C1772T polymorphism and the other 1 to determine the immunohistochemical expression of HIF-1α, VEGF, as well as CD 34 proteins. The comparison of polymorphism frequency between study and control population was conducted by collecting a 5 mL of peripheral venous blood samples in each subject. Results Clinicopathological characteristics of laryngeal carcinoma didn’t affect the expression of hypoxia-related biomarkers, such as HIF-1α, VEGF or MVD. The statistically significant association between HIF-1α and VEGF expression was found ( P = .034), but not between HIF-1α expression and MVD value ( P = .696). The expression of HIF-1α was significantly higher among CT heterozygotes ( P = .029). We found a significantly more recurrence among CT heterozygotes compared with patients with CC homozygous alleles (57.10% and 24.30%, respectively; P = .007). Patients with C1772T polymorphic variants had significantly worse disease-free survival compared with patients without polymorphism (Log-rank test, P = .007). Conclusion HIF-1α C1772T polymorphism was significantly associated with worse disease-free survival which nominates it as a predictor of laryngeal carcinoma relapse. The preoperative assessment of hypoxia-related biomarkers should be used in everyday practice in order to determine the treatment modalities for laryngeal carcinoma.https://doi.org/10.1177/10732748221144457 |
| spellingShingle | Miljan M. Folic MD, PhD Ana V. Banko MD, PhD Vera N. Todorovic MD, PhD Nela S. Puskas MD, PhD Jovica P. Milovanovic MD, PhD Sanja B. Krejovic MD, PhD Neda Z. Dragicevic-Babic MD Zoran M. Bukumiric MD, PhD Biljana R. Milicic MD, PhD Ana D. Jotic MD, PhD Vojko B. Djukic MD, PhD The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1α C1772T Polymorphism as Predictor of Laryngeal Carcinoma Relapse Cancer Control |
| title | The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1α C1772T Polymorphism as Predictor of Laryngeal Carcinoma Relapse |
| title_full | The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1α C1772T Polymorphism as Predictor of Laryngeal Carcinoma Relapse |
| title_fullStr | The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1α C1772T Polymorphism as Predictor of Laryngeal Carcinoma Relapse |
| title_full_unstemmed | The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1α C1772T Polymorphism as Predictor of Laryngeal Carcinoma Relapse |
| title_short | The Expression of Hypoxia-Related Biomarkers: A Significance of HIF-1α C1772T Polymorphism as Predictor of Laryngeal Carcinoma Relapse |
| title_sort | expression of hypoxia related biomarkers a significance of hif 1α c1772t polymorphism as predictor of laryngeal carcinoma relapse |
| url | https://doi.org/10.1177/10732748221144457 |
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