Effectiveness of Natural Killer (NK) Cells in Peripheral Blood Stem-Cell towards Expression of EZH2, Ki-67, and Apoptosis in Retinoblastoma (RB) Cells Culture
A natural killer (NK) cell is a large granular lymphocyte having a cytotoxic activity against malignant tumor cells in the cellular immune response. NK cells play an important role to inhibit the metastasis of tumor and express glikoprotein molecule antigen (IgG) and variable cytotoxic factors like...
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| Format: | Article |
| Language: | English |
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Society of Turaz Bilim
2015-09-01
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| Series: | Medicine Science |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=173686 |
| _version_ | 1797327830477963264 |
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| author | Hendrian Dwikoloso Soebagjo Susy Fatmariyanti Delfitri Lutfi |
| author_facet | Hendrian Dwikoloso Soebagjo Susy Fatmariyanti Delfitri Lutfi |
| author_sort | Hendrian Dwikoloso Soebagjo |
| collection | DOAJ |
| description | A natural killer (NK) cell is a large granular lymphocyte having a cytotoxic activity against malignant tumor cells in the cellular immune response. NK cells play an important role to inhibit the metastasis of tumor and express glikoprotein molecule antigen (IgG) and variable cytotoxic factors like perforin, granzyme, cytolysin such as TNF-α against retinoblastoma cells. The aim of the study was to investigate the role of Natural Killer Cells (NK cells) as an alternative therapy, especially in cases of retinoblastoma. It is highly expected that the study can be used as a starting point in determining the strategic therapy, e.g. the cell targeted therapy to inhibit EZH2 and retinoblastoma cell proliferation. There were 2 groups of retinoblastoma cell cultures in this study, the groups of control (n=10) and exposure (n=10). The control group was a group of retinoblastoma cell cultures that did not get any therapy, while the exposure group was a group of retinoblastoma cell cultures with NK Cell exposure in vitro for 3 days. The data obtained were analyzed using the T- test and Pearson Correlation. In T-test, the results of the two groups indicated a significant difference in the positive expression of cell count of EZH2, Ki-67 and Apoptotic cells. The Pearson Correlation test showed that the expression of EZH2 was statistically negative significant (α: -0.930; p [Med-Science 2015; 4(3.000): 2414-30] |
| first_indexed | 2024-03-08T06:43:07Z |
| format | Article |
| id | doaj.art-35df844053bf473ab470995ff654dd4d |
| institution | Directory Open Access Journal |
| issn | 2147-0634 |
| language | English |
| last_indexed | 2024-03-08T06:43:07Z |
| publishDate | 2015-09-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj.art-35df844053bf473ab470995ff654dd4d2024-02-03T08:19:16ZengSociety of Turaz BilimMedicine Science2147-06342015-09-014324143010.5455/medscience.2015.04.8256173686Effectiveness of Natural Killer (NK) Cells in Peripheral Blood Stem-Cell towards Expression of EZH2, Ki-67, and Apoptosis in Retinoblastoma (RB) Cells CultureHendrian Dwikoloso Soebagjo0Susy Fatmariyanti1Delfitri Lutfi2Department of Ophthalmology, Faculty of Medicine, Airlangga University Dr.Soetomo General Hospital Surabaya, Prof. Dr. Moestopo 6-8 Road, Surabaya, East Java, Indonesia. Department of Ophthalmology, Faculty of Medicine, Airlangga UniversityA natural killer (NK) cell is a large granular lymphocyte having a cytotoxic activity against malignant tumor cells in the cellular immune response. NK cells play an important role to inhibit the metastasis of tumor and express glikoprotein molecule antigen (IgG) and variable cytotoxic factors like perforin, granzyme, cytolysin such as TNF-α against retinoblastoma cells. The aim of the study was to investigate the role of Natural Killer Cells (NK cells) as an alternative therapy, especially in cases of retinoblastoma. It is highly expected that the study can be used as a starting point in determining the strategic therapy, e.g. the cell targeted therapy to inhibit EZH2 and retinoblastoma cell proliferation. There were 2 groups of retinoblastoma cell cultures in this study, the groups of control (n=10) and exposure (n=10). The control group was a group of retinoblastoma cell cultures that did not get any therapy, while the exposure group was a group of retinoblastoma cell cultures with NK Cell exposure in vitro for 3 days. The data obtained were analyzed using the T- test and Pearson Correlation. In T-test, the results of the two groups indicated a significant difference in the positive expression of cell count of EZH2, Ki-67 and Apoptotic cells. The Pearson Correlation test showed that the expression of EZH2 was statistically negative significant (α: -0.930; p [Med-Science 2015; 4(3.000): 2414-30]http://www.ejmanager.com/fulltextpdf.php?mno=173686NK cellsretinoblastomaEZH2proliferationapoptosis |
| spellingShingle | Hendrian Dwikoloso Soebagjo Susy Fatmariyanti Delfitri Lutfi Effectiveness of Natural Killer (NK) Cells in Peripheral Blood Stem-Cell towards Expression of EZH2, Ki-67, and Apoptosis in Retinoblastoma (RB) Cells Culture Medicine Science NK cells retinoblastoma EZH2 proliferation apoptosis |
| title | Effectiveness of Natural Killer (NK) Cells in Peripheral Blood Stem-Cell towards Expression of EZH2, Ki-67, and Apoptosis in Retinoblastoma (RB) Cells Culture |
| title_full | Effectiveness of Natural Killer (NK) Cells in Peripheral Blood Stem-Cell towards Expression of EZH2, Ki-67, and Apoptosis in Retinoblastoma (RB) Cells Culture |
| title_fullStr | Effectiveness of Natural Killer (NK) Cells in Peripheral Blood Stem-Cell towards Expression of EZH2, Ki-67, and Apoptosis in Retinoblastoma (RB) Cells Culture |
| title_full_unstemmed | Effectiveness of Natural Killer (NK) Cells in Peripheral Blood Stem-Cell towards Expression of EZH2, Ki-67, and Apoptosis in Retinoblastoma (RB) Cells Culture |
| title_short | Effectiveness of Natural Killer (NK) Cells in Peripheral Blood Stem-Cell towards Expression of EZH2, Ki-67, and Apoptosis in Retinoblastoma (RB) Cells Culture |
| title_sort | effectiveness of natural killer nk cells in peripheral blood stem cell towards expression of ezh2 ki 67 and apoptosis in retinoblastoma rb cells culture |
| topic | NK cells retinoblastoma EZH2 proliferation apoptosis |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=173686 |
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