Physical Interactions between Yeast Pichia guilliermondii and Post-Harvest Fruit Pathogen Penicillium expansum
Attachment of yeast cells or bacteria on fungal hyphae have been observed in various antagonisms between microorganisms. Physical interactions between yeast Pichia guilliermondii and postharvest fruit pathogen Penicillium expansum in culture were studied in detail using light and transmission electr...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2008-03-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916302637 |
| _version_ | 1819090285339082752 |
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| author | SRI WIDYASTUTI |
| author_facet | SRI WIDYASTUTI |
| author_sort | SRI WIDYASTUTI |
| collection | DOAJ |
| description | Attachment of yeast cells or bacteria on fungal hyphae have been observed in various antagonisms between microorganisms. Physical interactions between yeast Pichia guilliermondii and postharvest fruit pathogen Penicillium expansum in culture were studied in detail using light and transmission electron microscope to give better understanding on their mode of antagonism. Both organisms were co-cultured for 24-hr on potato dextrose agar. Light microscopy observations on the co-culture showed that the yeast cells attached firmly on the fungal hyphae. This attachment was inhibited by several substances such as enzymes degrading protein (protease or trypsin), a respiration inhibitor (sodium azide), an acid (hydrochloric acid) or an alkali (sodium hydroxide). Although autoclaved hyphae did not affect the attachment, but boiled enzymes and autoclaved yeast cells totally abolished the attachment. These evidences suggested that the attachment might be an active process mediated by certain protein from live yeast cells. Transmission electron micrographs on the ultrastructure of the co-culture revealed that the hyphae showed abnormalities in their structure and organelles, and a degree of obvious damage. Physical interactions observed in this study could be contributed to the mechanism of antagonism between P. guilliermondii and P. expansum. |
| first_indexed | 2024-12-21T22:21:24Z |
| format | Article |
| id | doaj.art-35dfeeac3ee94419bfa7c4a65464b88d |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-21T22:21:24Z |
| publishDate | 2008-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-35dfeeac3ee94419bfa7c4a65464b88d2022-12-21T18:48:19ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192008-03-01151273110.4308/hjb.15.1.27Physical Interactions between Yeast Pichia guilliermondii and Post-Harvest Fruit Pathogen Penicillium expansumSRI WIDYASTUTIAttachment of yeast cells or bacteria on fungal hyphae have been observed in various antagonisms between microorganisms. Physical interactions between yeast Pichia guilliermondii and postharvest fruit pathogen Penicillium expansum in culture were studied in detail using light and transmission electron microscope to give better understanding on their mode of antagonism. Both organisms were co-cultured for 24-hr on potato dextrose agar. Light microscopy observations on the co-culture showed that the yeast cells attached firmly on the fungal hyphae. This attachment was inhibited by several substances such as enzymes degrading protein (protease or trypsin), a respiration inhibitor (sodium azide), an acid (hydrochloric acid) or an alkali (sodium hydroxide). Although autoclaved hyphae did not affect the attachment, but boiled enzymes and autoclaved yeast cells totally abolished the attachment. These evidences suggested that the attachment might be an active process mediated by certain protein from live yeast cells. Transmission electron micrographs on the ultrastructure of the co-culture revealed that the hyphae showed abnormalities in their structure and organelles, and a degree of obvious damage. Physical interactions observed in this study could be contributed to the mechanism of antagonism between P. guilliermondii and P. expansum.http://www.sciencedirect.com/science/article/pii/S1978301916302637Pichia guilliermondiiPénicillium expansumattachmenttransmission electron microscope |
| spellingShingle | SRI WIDYASTUTI Physical Interactions between Yeast Pichia guilliermondii and Post-Harvest Fruit Pathogen Penicillium expansum Hayati Journal of Biosciences Pichia guilliermondii Pénicillium expansum attachment transmission electron microscope |
| title | Physical Interactions between Yeast Pichia guilliermondii and Post-Harvest Fruit Pathogen Penicillium expansum |
| title_full | Physical Interactions between Yeast Pichia guilliermondii and Post-Harvest Fruit Pathogen Penicillium expansum |
| title_fullStr | Physical Interactions between Yeast Pichia guilliermondii and Post-Harvest Fruit Pathogen Penicillium expansum |
| title_full_unstemmed | Physical Interactions between Yeast Pichia guilliermondii and Post-Harvest Fruit Pathogen Penicillium expansum |
| title_short | Physical Interactions between Yeast Pichia guilliermondii and Post-Harvest Fruit Pathogen Penicillium expansum |
| title_sort | physical interactions between yeast pichia guilliermondii and post harvest fruit pathogen penicillium expansum |
| topic | Pichia guilliermondii Pénicillium expansum attachment transmission electron microscope |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916302637 |
| work_keys_str_mv | AT sriwidyastuti physicalinteractionsbetweenyeastpichiaguilliermondiiandpostharvestfruitpathogenpenicilliumexpansum |