Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma
Background The X-linked tumor suppressor gene LDOC1 is reported to be involved in oral cancer. The detection of biomarkers in salivary RNA is a non-invasive strategy for diagnosing many diseases. The aim of the present study was to investigate the potential of salivary LDOC1 as a biomarker of oral c...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2019-04-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/6732.pdf |
| _version_ | 1827610967858479104 |
|---|---|
| author | Chung-Ji Liu Jen-Hao Chen Shih-Min Hsia Chiu-Chu Liao Hui-Wen Chang Tzong-Ming Shieh Yin-Hwa Shih |
| author_facet | Chung-Ji Liu Jen-Hao Chen Shih-Min Hsia Chiu-Chu Liao Hui-Wen Chang Tzong-Ming Shieh Yin-Hwa Shih |
| author_sort | Chung-Ji Liu |
| collection | DOAJ |
| description | Background The X-linked tumor suppressor gene LDOC1 is reported to be involved in oral cancer. The detection of biomarkers in salivary RNA is a non-invasive strategy for diagnosing many diseases. The aim of the present study was to investigate the potential of salivary LDOC1 as a biomarker of oral cancer. Methods We determined the expression levels of LDOC1 in the saliva of oral squamous cell carcinoma (OSCC) subjects, and investigated its correlation with various clinicopathological characteristics. The expression levels of salivary LDOC1 were detected in 53 OSCC subjects and 43 healthy controls using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. We used Fisher’s exact test to analyze the correlations between expression levels and clinicopathological characteristics. Results Salivary LDOC1 was significantly upregulated in females with OSCC (p = 0.0072), and significantly downregulated in males with OSCC (p = 0.0206). Eighty-nine percent of male OSCC subjects who smoked expressed low levels of LDOC1. OSCC cell lines derived from male OSCC subjects expressed low levels of LDOC1. Conclusions A high level of salivary LDOC1 expression is a biomarker of OSCC in females. A high percentage of male OSCC subjects who smoke express low levels of salivary LDOC1. A low level of salivary LDOC1 expression is a biomarker of OSCC in males. |
| first_indexed | 2024-03-09T07:58:20Z |
| format | Article |
| id | doaj.art-35e17a8ee1a8471fa20512311c20dadb |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:58:20Z |
| publishDate | 2019-04-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-35e17a8ee1a8471fa20512311c20dadb2023-12-03T00:49:27ZengPeerJ Inc.PeerJ2167-83592019-04-017e673210.7717/peerj.6732Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinomaChung-Ji Liu0Jen-Hao Chen1Shih-Min Hsia2Chiu-Chu Liao3Hui-Wen Chang4Tzong-Ming Shieh5Yin-Hwa Shih6Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei, TaiwanSchool of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, TaiwanDepartment of Healthcare Administration, Asia University, Taichung, TaiwanDepartment of Healthcare Administration, Asia University, Taichung, TaiwanDepartment of Dental Hygiene, College of Health Care, China Medical University, Taichung, TaiwanDepartment of Healthcare Administration, Asia University, Taichung, TaiwanBackground The X-linked tumor suppressor gene LDOC1 is reported to be involved in oral cancer. The detection of biomarkers in salivary RNA is a non-invasive strategy for diagnosing many diseases. The aim of the present study was to investigate the potential of salivary LDOC1 as a biomarker of oral cancer. Methods We determined the expression levels of LDOC1 in the saliva of oral squamous cell carcinoma (OSCC) subjects, and investigated its correlation with various clinicopathological characteristics. The expression levels of salivary LDOC1 were detected in 53 OSCC subjects and 43 healthy controls using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. We used Fisher’s exact test to analyze the correlations between expression levels and clinicopathological characteristics. Results Salivary LDOC1 was significantly upregulated in females with OSCC (p = 0.0072), and significantly downregulated in males with OSCC (p = 0.0206). Eighty-nine percent of male OSCC subjects who smoked expressed low levels of LDOC1. OSCC cell lines derived from male OSCC subjects expressed low levels of LDOC1. Conclusions A high level of salivary LDOC1 expression is a biomarker of OSCC in females. A high percentage of male OSCC subjects who smoke express low levels of salivary LDOC1. A low level of salivary LDOC1 expression is a biomarker of OSCC in males.https://peerj.com/articles/6732.pdfSalivary RNALDOC1BiomarkerOral squamous cell carcinoma |
| spellingShingle | Chung-Ji Liu Jen-Hao Chen Shih-Min Hsia Chiu-Chu Liao Hui-Wen Chang Tzong-Ming Shieh Yin-Hwa Shih Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma PeerJ Salivary RNA LDOC1 Biomarker Oral squamous cell carcinoma |
| title | Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma |
| title_full | Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma |
| title_fullStr | Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma |
| title_full_unstemmed | Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma |
| title_short | Salivary LDOC1 is a gender-difference biomarker of oral squamous cell carcinoma |
| title_sort | salivary ldoc1 is a gender difference biomarker of oral squamous cell carcinoma |
| topic | Salivary RNA LDOC1 Biomarker Oral squamous cell carcinoma |
| url | https://peerj.com/articles/6732.pdf |
| work_keys_str_mv | AT chungjiliu salivaryldoc1isagenderdifferencebiomarkeroforalsquamouscellcarcinoma AT jenhaochen salivaryldoc1isagenderdifferencebiomarkeroforalsquamouscellcarcinoma AT shihminhsia salivaryldoc1isagenderdifferencebiomarkeroforalsquamouscellcarcinoma AT chiuchuliao salivaryldoc1isagenderdifferencebiomarkeroforalsquamouscellcarcinoma AT huiwenchang salivaryldoc1isagenderdifferencebiomarkeroforalsquamouscellcarcinoma AT tzongmingshieh salivaryldoc1isagenderdifferencebiomarkeroforalsquamouscellcarcinoma AT yinhwashih salivaryldoc1isagenderdifferencebiomarkeroforalsquamouscellcarcinoma |