Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method
Taurine (2-aminoethanesulfonic acid) is a free sulfur-containing β-amino acid widely distributed in many mammalians. Owing to the energizing effects, it is mostly used in soft drinks and supplements for athletes. Regular intake of soft drinks may lead to an overdose of caffeine, taurine, and guarana...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2023-08-01
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| Series: | European Pharmaceutical Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/afpuc-2023-0010 |
| _version_ | 1797303159985537024 |
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| author | Chalova P. Salaskova D. Csicsay F. Galba J. Kovac A. Piestansky J. |
| author_facet | Chalova P. Salaskova D. Csicsay F. Galba J. Kovac A. Piestansky J. |
| author_sort | Chalova P. |
| collection | DOAJ |
| description | Taurine (2-aminoethanesulfonic acid) is a free sulfur-containing β-amino acid widely distributed in many mammalians. Owing to the energizing effects, it is mostly used in soft drinks and supplements for athletes. Regular intake of soft drinks may lead to an overdose of caffeine, taurine, and guarana and loss of bone mass, overweight, hypertension, and in older age, osteoporosis and cardiovascular diseases. Therefore, it is essential to control the maximum amount of taurine consumed by humans in the food and beverages. Here, a fast, simple, accurate, and robust method based on ultrahigh-performance liquid chromatography hyphenated with mass spectrometry (UHPLC-MS) was successfully applied for the determination of taurine in selected soft drinks sold in Slovakia. The method was characterized by coefficient of determination higher than 0.99, and the predicted value of the limit of detection was 4.29 μmol/L. The analyzed levels of taurine in selected commercial drinks ranged from 2.8 to 3.78 mg/mL. The concentration in one brand of the investigated drinks was found to be extremely low (about 70%) compared to the declared content by the manufacturer. |
| first_indexed | 2024-03-07T23:48:56Z |
| format | Article |
| id | doaj.art-35e1e0e487784df3939d7ce380b8cefc |
| institution | Directory Open Access Journal |
| issn | 2453-6725 |
| language | English |
| last_indexed | 2024-03-07T23:48:56Z |
| publishDate | 2023-08-01 |
| publisher | Sciendo |
| record_format | Article |
| series | European Pharmaceutical Journal |
| spelling | doaj.art-35e1e0e487784df3939d7ce380b8cefc2024-02-19T09:03:14ZengSciendoEuropean Pharmaceutical Journal2453-67252023-08-0170s1677410.2478/afpuc-2023-0010Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry methodChalova P.0Salaskova D.1Csicsay F.2Galba J.3Kovac A.4Piestansky J.5Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojarov 10, SK-832 32Bratislava, Slovak RepublicDepartment of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojarov 10, SK-832 32Bratislava, Slovak RepublicBiomedical Research Center Slovak Academy of Sciences, Institute of Virology, Dubravska cesta 9, SK-845 05Bratislava, Slovak RepublicBiomedical Research Center Slovak Academy of Sciences, Institute of Virology, Dubravska cesta 9, SK-845 05Bratislava, Slovak RepublicInstitute of Neuroimmunology, Slovak Academy of Sciences, Dubravska cesta 9, SK-845 10Bratislava, Slovak RepublicInstitute of Neuroimmunology, Slovak Academy of Sciences, Dubravska cesta 9, SK-845 10Bratislava, Slovak RepublicTaurine (2-aminoethanesulfonic acid) is a free sulfur-containing β-amino acid widely distributed in many mammalians. Owing to the energizing effects, it is mostly used in soft drinks and supplements for athletes. Regular intake of soft drinks may lead to an overdose of caffeine, taurine, and guarana and loss of bone mass, overweight, hypertension, and in older age, osteoporosis and cardiovascular diseases. Therefore, it is essential to control the maximum amount of taurine consumed by humans in the food and beverages. Here, a fast, simple, accurate, and robust method based on ultrahigh-performance liquid chromatography hyphenated with mass spectrometry (UHPLC-MS) was successfully applied for the determination of taurine in selected soft drinks sold in Slovakia. The method was characterized by coefficient of determination higher than 0.99, and the predicted value of the limit of detection was 4.29 μmol/L. The analyzed levels of taurine in selected commercial drinks ranged from 2.8 to 3.78 mg/mL. The concentration in one brand of the investigated drinks was found to be extremely low (about 70%) compared to the declared content by the manufacturer.https://doi.org/10.2478/afpuc-2023-0010taurinesoft drinksliquid chromatographyfood and food supplements quality controlmass spectrometry |
| spellingShingle | Chalova P. Salaskova D. Csicsay F. Galba J. Kovac A. Piestansky J. Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method European Pharmaceutical Journal taurine soft drinks liquid chromatography food and food supplements quality control mass spectrometry |
| title | Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method |
| title_full | Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method |
| title_fullStr | Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method |
| title_full_unstemmed | Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method |
| title_short | Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method |
| title_sort | determination of taurine in soft drinks by an ultrahigh performance liquid chromatography mass spectrometry method |
| topic | taurine soft drinks liquid chromatography food and food supplements quality control mass spectrometry |
| url | https://doi.org/10.2478/afpuc-2023-0010 |
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