Topoisomerase I prevents transcription-replication conflicts at transcription termination sites

Topoisomerase I prevents transcription-replication conflicts at transcription termination sites

R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the trans...

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Bibliographic Details
Main Authors: Yaqun Liu, Yea-Lih Lin, Philippe Pasero, Chun-Long Chen
Format: Article
Language:English
Published: Taylor & Francis Group 2021-01-01
Series:Molecular & Cellular Oncology
Subjects:
transcription-replication conflict
r-loops
topoisomerase i
chromosome breaks
Online Access:http://dx.doi.org/10.1080/23723556.2020.1843951
Description
Summary:R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the transcription termination sites of highly expressed genes enriched in R-loops and prevents head-on conflicts with transcription, in a topoisomerase I-dependent manner.
ISSN:2372-3556

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