Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the trans...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-01-01
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| Series: | Molecular & Cellular Oncology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23723556.2020.1843951 |
| _version_ | 1797677137936777216 |
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| author | Yaqun Liu Yea-Lih Lin Philippe Pasero Chun-Long Chen |
| author_facet | Yaqun Liu Yea-Lih Lin Philippe Pasero Chun-Long Chen |
| author_sort | Yaqun Liu |
| collection | DOAJ |
| description | R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the transcription termination sites of highly expressed genes enriched in R-loops and prevents head-on conflicts with transcription, in a topoisomerase I-dependent manner. |
| first_indexed | 2024-03-11T22:39:38Z |
| format | Article |
| id | doaj.art-35e4cba44cab42719755eb47f371e731 |
| institution | Directory Open Access Journal |
| issn | 2372-3556 |
| language | English |
| last_indexed | 2024-03-11T22:39:38Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Molecular & Cellular Oncology |
| spelling | doaj.art-35e4cba44cab42719755eb47f371e7312023-09-22T09:11:02ZengTaylor & Francis GroupMolecular & Cellular Oncology2372-35562021-01-018110.1080/23723556.2020.18439511843951Topoisomerase I prevents transcription-replication conflicts at transcription termination sitesYaqun Liu0Yea-Lih Lin1Philippe Pasero2Chun-Long Chen3PSL Research University, CNRS, UMR3244, Sorbonne UniversitéCNRS et Université de MontpellierCNRS et Université de MontpellierPSL Research University, CNRS, UMR3244, Sorbonne UniversitéR-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the transcription termination sites of highly expressed genes enriched in R-loops and prevents head-on conflicts with transcription, in a topoisomerase I-dependent manner.http://dx.doi.org/10.1080/23723556.2020.1843951transcription-replication conflictr-loopstopoisomerase ichromosome breaks |
| spellingShingle | Yaqun Liu Yea-Lih Lin Philippe Pasero Chun-Long Chen Topoisomerase I prevents transcription-replication conflicts at transcription termination sites Molecular & Cellular Oncology transcription-replication conflict r-loops topoisomerase i chromosome breaks |
| title | Topoisomerase I prevents transcription-replication conflicts at transcription termination sites |
| title_full | Topoisomerase I prevents transcription-replication conflicts at transcription termination sites |
| title_fullStr | Topoisomerase I prevents transcription-replication conflicts at transcription termination sites |
| title_full_unstemmed | Topoisomerase I prevents transcription-replication conflicts at transcription termination sites |
| title_short | Topoisomerase I prevents transcription-replication conflicts at transcription termination sites |
| title_sort | topoisomerase i prevents transcription replication conflicts at transcription termination sites |
| topic | transcription-replication conflict r-loops topoisomerase i chromosome breaks |
| url | http://dx.doi.org/10.1080/23723556.2020.1843951 |
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