IS-linked movement of a restriction-modification system.
Potential mobility of restriction-modification systems has been suggested by evolutionary/bioinformatic analysis of prokaryotic genomes. Here we demonstrate in vivo movement of a restriction-modification system within a genome under a laboratory condition. After blocking replication of a temperature...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3031569?pdf=render |
| _version_ | 1818031045373591552 |
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| author | Noriko Takahashi Seishi Ohashi Marat R Sadykov Yoko Mizutani-Ui Ichizo Kobayashi |
| author_facet | Noriko Takahashi Seishi Ohashi Marat R Sadykov Yoko Mizutani-Ui Ichizo Kobayashi |
| author_sort | Noriko Takahashi |
| collection | DOAJ |
| description | Potential mobility of restriction-modification systems has been suggested by evolutionary/bioinformatic analysis of prokaryotic genomes. Here we demonstrate in vivo movement of a restriction-modification system within a genome under a laboratory condition. After blocking replication of a temperature-sensitive plasmid carrying a PaeR7I restriction-modification system in Escherichia coli cells, the plasmid was found integrated into the chromosome of the surviving cells. Sequence analysis revealed that, in the majority of products, the restriction-modification system was linked to chromosomal insertion sequences (ISs). Three types of products were: (I) apparent co-integration of the plasmid and the chromosome at a chromosomal IS1 or IS5 copy (24/28 analyzed); (II) de novo insertion of IS1 with the entire plasmid except for a 1-3 bp terminal deletion (2/28); and (III) reciprocal crossing-over between the plasmid and the chromosome involving 1-3 bp of sequence identity (2/28). An R-negative mutation apparently decreased the efficiency of successful integration by two orders of magnitude. Reconstruction experiments demonstrated that the restriction-dependence was mainly due to selection against cells without proper integration: their growth was inhibited by the restriction enzyme action. These results demonstrate collaboration of a mobile element and a restriction-modification system for successful joint migration. This collaboration may have promoted the spread and, therefore, the long-term persistence of these complexes and restriction-modification systems in a wide range of prokaryotes. |
| first_indexed | 2024-12-10T05:45:14Z |
| format | Article |
| id | doaj.art-35ea1e560a774bf3a29f9073f255bdc1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:45:14Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-35ea1e560a774bf3a29f9073f255bdc12022-12-22T02:00:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1655410.1371/journal.pone.0016554IS-linked movement of a restriction-modification system.Noriko TakahashiSeishi OhashiMarat R SadykovYoko Mizutani-UiIchizo KobayashiPotential mobility of restriction-modification systems has been suggested by evolutionary/bioinformatic analysis of prokaryotic genomes. Here we demonstrate in vivo movement of a restriction-modification system within a genome under a laboratory condition. After blocking replication of a temperature-sensitive plasmid carrying a PaeR7I restriction-modification system in Escherichia coli cells, the plasmid was found integrated into the chromosome of the surviving cells. Sequence analysis revealed that, in the majority of products, the restriction-modification system was linked to chromosomal insertion sequences (ISs). Three types of products were: (I) apparent co-integration of the plasmid and the chromosome at a chromosomal IS1 or IS5 copy (24/28 analyzed); (II) de novo insertion of IS1 with the entire plasmid except for a 1-3 bp terminal deletion (2/28); and (III) reciprocal crossing-over between the plasmid and the chromosome involving 1-3 bp of sequence identity (2/28). An R-negative mutation apparently decreased the efficiency of successful integration by two orders of magnitude. Reconstruction experiments demonstrated that the restriction-dependence was mainly due to selection against cells without proper integration: their growth was inhibited by the restriction enzyme action. These results demonstrate collaboration of a mobile element and a restriction-modification system for successful joint migration. This collaboration may have promoted the spread and, therefore, the long-term persistence of these complexes and restriction-modification systems in a wide range of prokaryotes.http://europepmc.org/articles/PMC3031569?pdf=render |
| spellingShingle | Noriko Takahashi Seishi Ohashi Marat R Sadykov Yoko Mizutani-Ui Ichizo Kobayashi IS-linked movement of a restriction-modification system. PLoS ONE |
| title | IS-linked movement of a restriction-modification system. |
| title_full | IS-linked movement of a restriction-modification system. |
| title_fullStr | IS-linked movement of a restriction-modification system. |
| title_full_unstemmed | IS-linked movement of a restriction-modification system. |
| title_short | IS-linked movement of a restriction-modification system. |
| title_sort | is linked movement of a restriction modification system |
| url | http://europepmc.org/articles/PMC3031569?pdf=render |
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