Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D
The common trait of all auxins is a stimulation of cell elongation and also cell division in the presence of cytokinin; both are essential for callus induction and its multiplication. The response of plant tissues to various compounds with auxin activity may be quite different. In this study, the ef...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2017-12-01
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| Series: | Acta Agrobotanica |
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| Online Access: | https://pbsociety.org.pl/journals/index.php/aa/article/view/7994 |
| _version_ | 1818059542064267264 |
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| author | Andrzej Gatz Magdalena Tomaszewska-Sowa Anna Figas |
| author_facet | Andrzej Gatz Magdalena Tomaszewska-Sowa Anna Figas |
| author_sort | Andrzej Gatz |
| collection | DOAJ |
| description | The common trait of all auxins is a stimulation of cell elongation and also cell division in the presence of cytokinin; both are essential for callus induction and its multiplication. The response of plant tissues to various compounds with auxin activity may be quite different. In this study, the effectiveness of a synthetic auxin, 2,4-dichlorofenoxyacetic acid (2,4-D), instead of the generally applied natural auxin, indole-3-acetic acid (IAA), was tested for the proliferation of Nicotiana tabacum callus. The following concentrations of 2,4-D were tested: 0.1, 0.5, 1.0, 1.5, and 2.0 mg dm−3. Callus was derived from stem pith and its proliferation allowed on MS medium through five subcultures at 25°C and in darkness. After each passage, the fresh weight and morphological features of the callus were determined. The 0.5 mg dm−3 2,4-D treatment was the most favorable for producing the greatest increase in fresh weight in each of five subsequent subcultures as well as maintaining normal morphological features for proliferation. However, the 1.0 mg dm−3 2,4-D treatment in comparison with the lowest, 0.1 mg dm−3, was more beneficial when considering regular increases of fresh weight and a better cell cohesion for callus growth. |
| first_indexed | 2024-12-10T13:18:10Z |
| format | Article |
| id | doaj.art-35ed971d1cb74874b862522609847c98 |
| institution | Directory Open Access Journal |
| issn | 2300-357X |
| language | English |
| last_indexed | 2024-12-10T13:18:10Z |
| publishDate | 2017-12-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Agrobotanica |
| spelling | doaj.art-35ed971d1cb74874b862522609847c982022-12-22T01:47:26ZengPolish Botanical SocietyActa Agrobotanica2300-357X2017-12-0170410.5586/aa.17256851Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-DAndrzej Gatz0Magdalena Tomaszewska-Sowa1Anna Figas2Laboratory of Plant Physiology and Fundamentals of Biotechnology, Department of Plant Genetics, Physiology and Biotechnology, UTP University of Science and Technology in Bydgoszcz, Bernardyńska 6, 85-029 BydgoszczLaboratory of Plant Physiology and Fundamentals of Biotechnology, Department of Plant Genetics, Physiology and Biotechnology, UTP University of Science and Technology in Bydgoszcz, Bernardyńska 6, 85-029 BydgoszczLaboratory of Plant Physiology and Fundamentals of Biotechnology, Department of Plant Genetics, Physiology and Biotechnology, UTP University of Science and Technology in Bydgoszcz, Bernardyńska 6, 85-029 BydgoszczThe common trait of all auxins is a stimulation of cell elongation and also cell division in the presence of cytokinin; both are essential for callus induction and its multiplication. The response of plant tissues to various compounds with auxin activity may be quite different. In this study, the effectiveness of a synthetic auxin, 2,4-dichlorofenoxyacetic acid (2,4-D), instead of the generally applied natural auxin, indole-3-acetic acid (IAA), was tested for the proliferation of Nicotiana tabacum callus. The following concentrations of 2,4-D were tested: 0.1, 0.5, 1.0, 1.5, and 2.0 mg dm−3. Callus was derived from stem pith and its proliferation allowed on MS medium through five subcultures at 25°C and in darkness. After each passage, the fresh weight and morphological features of the callus were determined. The 0.5 mg dm−3 2,4-D treatment was the most favorable for producing the greatest increase in fresh weight in each of five subsequent subcultures as well as maintaining normal morphological features for proliferation. However, the 1.0 mg dm−3 2,4-D treatment in comparison with the lowest, 0.1 mg dm−3, was more beneficial when considering regular increases of fresh weight and a better cell cohesion for callus growth.https://pbsociety.org.pl/journals/index.php/aa/article/view/7994Nicotiana tabacum2,4-dichlorophenoxyacetic acidproliferationcell divisionscallus morphology |
| spellingShingle | Andrzej Gatz Magdalena Tomaszewska-Sowa Anna Figas Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D Acta Agrobotanica Nicotiana tabacum 2,4-dichlorophenoxyacetic acid proliferation cell divisions callus morphology |
| title | Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D |
| title_full | Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D |
| title_fullStr | Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D |
| title_full_unstemmed | Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D |
| title_short | Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D |
| title_sort | changes accompanying proliferative capacity and morphology of nicotiana tabacum l callus in response to 2 4 d |
| topic | Nicotiana tabacum 2,4-dichlorophenoxyacetic acid proliferation cell divisions callus morphology |
| url | https://pbsociety.org.pl/journals/index.php/aa/article/view/7994 |
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